The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study

İlke Doğa Şeker; Tayfun Alacam; Gülçin Akca; Aysel Yılmaz; Sevgi Takka

Makale detayları

The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study

Yazarlar:

İlke Doğa Şeker | Tayfun Alacam | Gülçin Akca | Aysel Yılmaz | Sevgi Takka

Yayıncı:

Istanbul University

Ana Başlıklar:

Dentistry (Other)

Dergi:

European Oral Research Vol.59 Issue 1, pp.33-39

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001340872

pISSN:

2630-6158

Anahtar kelimeler:

Özet:

 Purpose: This study aimed to evaluate the effectiveness of R-limonene and its nano emulsion formulation against Enterococcus faecalis E. faecalis in infected root canals. Materials and Methods: A nano emulsion containing 20 R-limonene was prepared using the phase inversion method. E. faecalis was cultivated, and MICMBC values were determined through the macrodilution method. Standardized E. faecalis suspensions were used to infect the root canals ex vivo. Subsequently, R-limonene, nano emulsion containing 20 R-limonene, calcium hydroxide, and sterile saline were applied to the infected root canals for 7 days at the determined MICMBC dose. The root canals were sampled, and droplets were plated onto Mueller-Hinton agar plates, and viable bacteria Colony Forming Units, CFUmL were counted. Statistical analyzes were performed based on the data. Results: Statistically similar efficacy was observed between test materials on the root canal samples. All test materials were significantly more effective than the saline control p0.05, however, none of the test materials completely eliminated E. faecalis from root canals. Conclusion: Although the effects of R-limonene on E. faecalis in infected root canals appeared to be like calcium hydroxide, with its broad antibacterial spectrum, gutta-percha softening and cleansing benefits, it was considered that it could considered among antimicrobials that could be used especially in endodontic retreatments alone or in synergistic combinations.

 Purpose: This study aimed to evaluate the effectiveness of R-limonene and its nano emulsion formulation against Enterococcus faecalis E. faecalis in infected root canals. Materials and Methods: A nano emulsion containing 20 R-limonene was prepared using the phase inversion method. E. faecalis was cultivated, and MICMBC values were determined through the macrodilution method. Standardized E. faecalis suspensions were used to infect the root canals ex vivo. Subsequently, R-limonene, nano emulsion containing 20 R-limonene, calcium hydroxide, and sterile saline were applied to the infected root canals for 7 days at the determined MICMBC dose. The root canals were sampled, and droplets were plated onto Mueller-Hinton agar plates, and viable bacteria Colony Forming Units, CFUmL were counted. Statistical analyzes were performed based on the data. Results: Statistically similar efficacy was observed between test materials on the root canal samples. All test materials were significantly more effective than the saline control p0.05, however, none of the test materials completely eliminated E. faecalis from root canals. Conclusion: Although the effects of R-limonene on E. faecalis in infected root canals appeared to be like calcium hydroxide, with its broad antibacterial spectrum, gutta-percha softening and cleansing benefits, it was considered that it could considered among antimicrobials that could be used especially in endodontic retreatments alone or in synergistic combinations.

 Purpose: This study aimed to evaluate the effectiveness of R-limonene and its nano emulsion formulation against Enterococcus faecalis E. faecalis in infected root canals. Materials and Methods: A nano emulsion containing 20 R-limonene was prepared using the phase inversion method. E. faecalis was cultivated, and MICMBC values were determined through the macrodilution method. Standardized E. faecalis suspensions were used to infect the root canals ex vivo. Subsequently, R-limonene, nano emulsion containing 20 R-limonene, calcium hydroxide, and sterile saline were applied to the infected root canals for 7 days at the determined MICMBC dose. The root canals were sampled, and droplets were plated onto Mueller-Hinton agar plates, and viable bacteria Colony Forming Units, CFUmL were counted. Statistical analyzes were performed based on the data. Results: Statistically similar efficacy was observed between test materials on the root canal samples. All test materials were significantly more effective than the saline control p0.05, however, none of the test materials completely eliminated E. faecalis from root canals. Conclusion: Although the effects of R-limonene on E. faecalis in infected root canals appeared to be like calcium hydroxide, with its broad antibacterial spectrum, gutta-percha softening and cleansing benefits, it was considered that it could considered among antimicrobials that could be used especially in endodontic retreatments alone or in synergistic combinations.

 Purpose: This study aimed to evaluate the effectiveness of R-limonene and its nano emulsion formulation against Enterococcus faecalis E. faecalis in infected root canals. Materials and Methods: A nano emulsion containing 20 R-limonene was prepared using the phase inversion method. E. faecalis was cultivated, and MICMBC values were determined through the macrodilution method. Standardized E. faecalis suspensions were used to infect the root canals ex vivo. Subsequently, R-limonene, nano emulsion containing 20 R-limonene, calcium hydroxide, and sterile saline were applied to the infected root canals for 7 days at the determined MICMBC dose. The root canals were sampled, and droplets were plated onto Mueller-Hinton agar plates, and viable bacteria Colony Forming Units, CFUmL were counted. Statistical analyzes were performed based on the data. Results: Statistically similar efficacy was observed between test materials on the root canal samples. All test materials were significantly more effective than the saline control p0.05, however, none of the test materials completely eliminated E. faecalis from root canals. Conclusion: Although the effects of R-limonene on E. faecalis in infected root canals appeared to be like calcium hydroxide, with its broad antibacterial spectrum, gutta-percha softening and cleansing benefits, it was considered that it could considered among antimicrobials that could be used especially in endodontic retreatments alone or in synergistic combinations.

Makale detayları

The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study

Yazarlar:

İlke Doğa Şeker | Tayfun Alacam | Gülçin Akca | Aysel Yılmaz | Sevgi Takka

Yayıncı:

Istanbul University

Ana Başlıklar:

Dentistry (Other)

Dergi:

European Oral Research Vol.59 Issue 1, pp.33-39

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001340872

pISSN:

2630-6158

Antimicrobial effect

calcium hydroxide

Enterococcus faecalis

nano emulsion

R-limonene

Sayı

Makale detayları

The antimicrobial effect of R-limonene and its nano emulsion on Enterococcus faecalis - In vitro study

Yazarlar: İlke Doğa Şeker | Tayfun Alacam | Gülçin Akca | Aysel Yılmaz | Sevgi Takka

Yayıncı: Istanbul University

Ana Başlıklar: Dentistry (Other)

Dergi: European Oral Research Vol.59 Issue 1, pp.33-39

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001340872

pISSN: 2630-6158

Antimicrobial effect

calcium hydroxide

Enterococcus faecalis

nano emulsion

R-limonene

Sayı

Yazarlar:

İlke Doğa Şeker | Tayfun Alacam | Gülçin Akca | Aysel Yılmaz | Sevgi Takka

Yayıncı:

Istanbul University

Ana Başlıklar:

Dentistry (Other)

Dergi:

European Oral Research Vol.59 Issue 1, pp.33-39

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001340872

pISSN:

2630-6158