Effects of pH on the Genesis of Membrane Potential  Changes at Fertilization in the Egg of the Frog Rana  cameranoi

Şeref ERDOĞAN; Gülay LOGOĞLU; Kerem T. ÖZGÜNEN; T. ÖZGÜNEN

Makale detayları

Effects of pH on the Genesis of Membrane Potential Changes at Fertilization in the Egg of the Frog Rana cameranoi

Yazarlar:

Şeref ERDOĞAN | Gülay LOGOĞLU | Kerem T. ÖZGÜNEN | T. ÖZGÜNEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.725-736

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813928

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The apparent change in the egg membrane potential at fertilization gives rise to fertilization  potential (FP) . FP is the initial, transient electrical block to polyspermy as shown in most species  especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until  the permanent, mechanical block is set up. Polyspermy is lethal in most species, and for a successful  fertilization resting membrane potential (RMP) and FP of the egg have to be held in optimum ranges.  Shifts in environmental pH may interfere with a successful fertilization by affecting these bioelectrical  potentials. In the present study, we investigated how pH alterations affected the RMP and FP  parameters of the egg in the frog, Rana cameranoi. Egg membrane potentials were recorded by the  conventional microelectrode technique. RMP and FP parameters were evaluated. Ten percent Ringer  solution (pH=7.80) was used for the control group. Acidic (Acd) and alkaline (Alk) experimental groups  were kept in the Ringer solutions at pH 6.50 and 9.00, respectively.  RMP was found to be significantly more negative in the Acd group (p.

 

The apparent change in the egg membrane potential at fertilization gives rise to fertilization  potential (FP) . FP is the initial, transient electrical block to polyspermy as shown in most species  especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until  the permanent, mechanical block is set up. Polyspermy is lethal in most species, and for a successful  fertilization resting membrane potential (RMP) and FP of the egg have to be held in optimum ranges.  Shifts in environmental pH may interfere with a successful fertilization by affecting these bioelectrical  potentials. In the present study, we investigated how pH alterations affected the RMP and FP  parameters of the egg in the frog, Rana cameranoi. Egg membrane potentials were recorded by the  conventional microelectrode technique. RMP and FP parameters were evaluated. Ten percent Ringer  solution (pH=7.80) was used for the control group. Acidic (Acd) and alkaline (Alk) experimental groups  were kept in the Ringer solutions at pH 6.50 and 9.00, respectively.  RMP was found to be significantly more negative in the Acd group (p.

Makale detayları

Effects of pH on the Genesis of Membrane Potential Changes at Fertilization in the Egg of the Frog Rana cameranoi

Yazarlar:

Şeref ERDOĞAN | Gülay LOGOĞLU | Kerem T. ÖZGÜNEN | T. ÖZGÜNEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.725-736

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813928

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Resting membrane potential

fertilization potential

pH

frog egg.

Sayı

Makale detayları

Effects of pH on the Genesis of Membrane Potential Changes at Fertilization in the Egg of the Frog Rana cameranoi

Yazarlar: Şeref ERDOĞAN | Gülay LOGOĞLU | Kerem T. ÖZGÜNEN | T. ÖZGÜNEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.725-736

Belge Tipi: Makale

Yayın Tarihi: 3/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813928

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Resting membrane potential

fertilization potential

pH

frog egg.

Sayı

Yazarlar:

Şeref ERDOĞAN | Gülay LOGOĞLU | Kerem T. ÖZGÜNEN | T. ÖZGÜNEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 4, pp.725-736

Belge Tipi:

Makale

Yayın Tarihi:

3/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813928

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152