Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate

Anon THAMMASITTIRONG; Sudarat SAECHOW; Sutticha Na-ranong THAMMASITTIRONG

Makale detayları

Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate

Yazarlar:

Anon THAMMASITTIRONG | Sudarat SAECHOW | Sutticha Na-ranong THAMMASITTIRONG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.992-1002

Belge Tipi:

Makale

Yayın Tarihi:

11/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811316

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

The present study focused on the screening and optimization of biopolymer polyhydroxybutyrate (PHB) production by Bacillus spp. using cost-effective substrates. Among 602 local Bacillus isolates, Bacillus thuringiensis B417-5 produced the highest amount of PHB (2.278 g/L, 60.07% of dry cell weight, DCW). 1H NMR and FTIR analyses of the extracted polymer revealed the characteristic peaks of PHB. The optimization results showed that the highest PHB accumulation (2.768 g/L, 72.08% of DCW) was achieved when culturing B. thuringiensis B417-5 in a nitrogen-deficient medium containing 1% total sugar from sugarcane juice and 0.5% yeast extract, with a pH of 7.0 and an incubation temperature of 37 °C for 48 h. B. thuringiensis B417-5 can thus be considered a good candidate for large-scale production of PHB. We are reporting for the first time that sugarcane juice is a promising carbon source for economical PHB production by B. thuringiensis.

Makale detayları

Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate

Yazarlar: Anon THAMMASITTIRONG | Sudarat SAECHOW | Sutticha Na-ranong THAMMASITTIRONG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.992-1002

Belge Tipi: Makale

Yayın Tarihi: 11/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000811316

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Anon THAMMASITTIRONG | Sudarat SAECHOW | Sutticha Na-ranong THAMMASITTIRONG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2017 Vol.41 Issue 6, pp.992-1002

Belge Tipi:

Makale

Yayın Tarihi:

11/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000811316

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152