The Effects of Baicalin on Lipid peroxidation and Oxidative Damage in Rats Exposed to Cypermethrine

N.A.

Makale detayları

The Effects of Baicalin on Lipid peroxidation and Oxidative Damage in Rats Exposed to Cypermethrine

Yazarlar:

N.A.

Yayıncı:

Erciyes University

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.38-46

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287334

pISSN:

1304-7280

Anahtar kelimeler:

Özet:

 This study aims to investigate the protective effects of baicalin BAI in rats exposed to cypermethrin CYP. A total of 40 Wistar Albino male rats, 10 in each group, aged 6 to 8 weeks and weighing 180 to 220 g, were utilized for this experiment. The groups were determined, respectively, control, BAI 50 mg kg-1, CYP 25 mg kg-1, and CYP 25 mg kg-1  BAI 50 mg kg-1. At the end of the 21-day trial period, blood samples were collected from rats by cardiac puncture into test tubes with anticoagulant and non-anticoagulant properties under intraperitoneal ketamine and xylazine anesthesia. Afterward, the rats were sacrificed by cervical dislocation, and the liver, kidney, brain, testis, heart and lung tissues were removed. Glutathione, NO, and MDA levels and GPx, GR, GST, SOD, and CAT enzyme activities in tissue and blood plasma and erythrocyte samples were measured on a microplate reader by a spectrophotometric method, whereas LDH, AST, ALT, ALP, triglyceride, cholesterol, albumin, total protein, BUN, uric acid and creatinine levelsactivities in serum were measured on a Roche Cobas autoanalyzer. At the end of the experiment, it was determined that the parameters of the BAI group were similar to those of the control group P0.05. When the CYP- exposed group was compared to the control group, it was discovered that there was a statistically considerable de- crease in tissue GSH levels as well as GPx, GR, GST, SOD, and CAT enzyme activities P0.05. A significant in- crease was recorded in MDA and NO levels P0.05. Additionally, while serum total protein and albumin levels de- creased, AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levelsactivities increased P0.05. The effects of CYP were partially or completely ameliorated in the CYP and BAI co-administered group. Consequently, it was revealed that BAI may have a protective effect against CYP-induced oxidative damage in rats by suppressing oxidative stress and promoting antioxidant system activity.

 This study aims to investigate the protective effects of baicalin BAI in rats exposed to cypermethrin CYP. A total of 40 Wistar Albino male rats, 10 in each group, aged 6 to 8 weeks and weighing 180 to 220 g, were utilized for this experiment. The groups were determined, respectively, control, BAI 50 mg kg-1, CYP 25 mg kg-1, and CYP 25 mg kg-1  BAI 50 mg kg-1. At the end of the 21-day trial period, blood samples were collected from rats by cardiac puncture into test tubes with anticoagulant and non-anticoagulant properties under intraperitoneal ketamine and xylazine anesthesia. Afterward, the rats were sacrificed by cervical dislocation, and the liver, kidney, brain, testis, heart and lung tissues were removed. Glutathione, NO, and MDA levels and GPx, GR, GST, SOD, and CAT enzyme activities in tissue and blood plasma and erythrocyte samples were measured on a microplate reader by a spectrophotometric method, whereas LDH, AST, ALT, ALP, triglyceride, cholesterol, albumin, total protein, BUN, uric acid and creatinine levelsactivities in serum were measured on a Roche Cobas autoanalyzer. At the end of the experiment, it was determined that the parameters of the BAI group were similar to those of the control group P0.05. When the CYP- exposed group was compared to the control group, it was discovered that there was a statistically considerable de- crease in tissue GSH levels as well as GPx, GR, GST, SOD, and CAT enzyme activities P0.05. A significant in- crease was recorded in MDA and NO levels P0.05. Additionally, while serum total protein and albumin levels de- creased, AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levelsactivities increased P0.05. The effects of CYP were partially or completely ameliorated in the CYP and BAI co-administered group. Consequently, it was revealed that BAI may have a protective effect against CYP-induced oxidative damage in rats by suppressing oxidative stress and promoting antioxidant system activity.

 This study aims to investigate the protective effects of baicalin BAI in rats exposed to cypermethrin CYP. A total of 40 Wistar Albino male rats, 10 in each group, aged 6 to 8 weeks and weighing 180 to 220 g, were utilized for this experiment. The groups were determined, respectively, control, BAI 50 mg kg-1, CYP 25 mg kg-1, and CYP 25 mg kg-1  BAI 50 mg kg-1. At the end of the 21-day trial period, blood samples were collected from rats by cardiac puncture into test tubes with anticoagulant and non-anticoagulant properties under intraperitoneal ketamine and xylazine anesthesia. Afterward, the rats were sacrificed by cervical dislocation, and the liver, kidney, brain, testis, heart and lung tissues were removed. Glutathione, NO, and MDA levels and GPx, GR, GST, SOD, and CAT enzyme activities in tissue and blood plasma and erythrocyte samples were measured on a microplate reader by a spectrophotometric method, whereas LDH, AST, ALT, ALP, triglyceride, cholesterol, albumin, total protein, BUN, uric acid and creatinine levelsactivities in serum were measured on a Roche Cobas autoanalyzer. At the end of the experiment, it was determined that the parameters of the BAI group were similar to those of the control group P0.05. When the CYP- exposed group was compared to the control group, it was discovered that there was a statistically considerable de- crease in tissue GSH levels as well as GPx, GR, GST, SOD, and CAT enzyme activities P0.05. A significant in- crease was recorded in MDA and NO levels P0.05. Additionally, while serum total protein and albumin levels de- creased, AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levelsactivities increased P0.05. The effects of CYP were partially or completely ameliorated in the CYP and BAI co-administered group. Consequently, it was revealed that BAI may have a protective effect against CYP-induced oxidative damage in rats by suppressing oxidative stress and promoting antioxidant system activity.

 Bu çalışmada sipermetrin SPM maruz kalan sıçanlarda baikalinin BAİ koruyucu etkilerinin araştırılması amaç- landı. Çalışmada her grupta 10 tane olacak şekilde toplam 40 adet 6-8 haftalık 180-220 g ağırlığında Wistar Albino ırkı erkek sıçan kullanıldı. Gruplar sırasıyla kontrol, BAİ 50 mg kg-1, SPM 25 mg kg-1 ve SPM 25 mg kg-1  BAİ 50 mg kg-1 olarak belirlendi. 21 günlük deneme süresinin sonunda intraperitoneal ketamin-ksilazin anestezisi altında kalbe punksiyon yapılarak heparinize ve antikoagulansız özellikteki test tüplerine kan örneği alındı. Servikal dislokasyon son- rası sıçanlardan karaciğer, böbrek, beyin, testis, kalp ve akciğer dokuları çıkarıldı. Doku ve kan plazma ve eritrosit örneklerinde GSH, NO ve MDA düzeyleri ile GPx, GR, GST, SOD ve CAT enzim aktiviteleri mikroplaka okuyucuda spekrofotometrik yöntemle ölçülürken serumda LDH, AST, ALT ve ALP enzim aktiviteleri ile trigliserit, kolesterol, albü- min, total protein, BUN, ürik asit ve kreatinin düzeyleri Roche Cobas otoanalizöründe ölçüldü. Çalışma sonunda BAİ uygulanan grubun parametrelerinin kontrol grubuna benzer olduğu görüldü P0.05. Sipermetrin uygulanan grupta kontrole kıyasla doku GSH düzeyleri ile GPx, GR, GST, SOD ve CAT enzim aktivitelerinde anlamlı bir azalma tespit edildi P0.05. Malondialdehit ve NO seviyelerinde ise anlamlı bir artış kaydedildi P0.05. Ayrıca serum AST, ALT, ALP, LDH, kolesterol, trigliserit, BUN, ürik asit ve kreatinin düzeyaktivitelerinde artış görülürken serum total protein ve albümin düzeylerinde azalma görüldü P0.05. Sipermetrin ile BAİnin birlikte uygulandığı gruplarda SPMnin etkileri kısmen ya da tamamen iyileştirdi. Sonuç olarak, BAİnin sıçanlarda oksidatif stresi baskılayarak ve antioksidan sistem aktivitesini artırarak SPMnin neden olduğu oksidatif hasara karşı koruyucu etkiye sahip olabileceği ortaya konuldu.

Makale detayları

The Effects of Baicalin on Lipid peroxidation and Oxidative Damage in Rats Exposed to Cypermethrine

Yazarlar:

N.A.

Yayıncı:

Erciyes University

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.38-46

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287334

pISSN:

1304-7280

Baicalin

cypermethrine

lipid peroxidation

oxidative stress

pyrethroid

Baikalin

lipid peroksidasyon

oksidatif stres

piretroid

sipermetrin

Sayı

Makale detayları

Yazarlar: N.A.

Yayıncı: Erciyes University

Dergi: Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.38-46

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001287334

pISSN: 1304-7280

Sayı

Yazarlar:

N.A.

Yayıncı:

Erciyes University

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.38-46

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001287334

pISSN:

1304-7280