Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste

Neslihan Dikbaş; Waleed Salih Salman Al Dahluz; Şeyma Alım; Sevda Uçar

Makale detayları

Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste

Yazarlar:

Neslihan Dikbaş | Waleed Salih Salman Al Dahluz | Şeyma Alım | Sevda Uçar

Yayıncı:

Bingol University

Ana Başlıklar:

Enzimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.62-66

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328015

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 Cellulose, one of the most abundant carbohydrates on Earth, is a promising candidate for the production of second-generation biofuels such as ethanol and various everyday products. This polysaccharide is degraded by the enzyme cellulase, which is usually produced by microorganisms. Microbial cellulases are widely used in various industries such as textiles, detergents, pharmaceuticals, food and paper. In the present study, cellulase enzyme was partially purified from Bacillus pumilus ND8 strain isolated from garden waste and the pH and temperature values at which the enzyme showed optimum activity were determined. B. pumilus ND8 strain exhibited a cellulase activity of 8.6 UmL as a result of partial purification and the protein concentration of the enzyme was measured as 6.72 mgmL. The pH and temperature values at which the partially purified cellulase showed optimum activity were pH 5.5 and 60 C, respectively. Km and Vmax values of the enzyme were determined as 0.81 mM and 14.2 µmolmin, respectively. In conclusion, cellulase purified from B. pumilus ND8 strain was found to possess unique properties that make it suitable for industrial applications.

 Cellulose, one of the most abundant carbohydrates on Earth, is a promising candidate for the production of second-generation biofuels such as ethanol and various everyday products. This polysaccharide is degraded by the enzyme cellulase, which is usually produced by microorganisms. Microbial cellulases are widely used in various industries such as textiles, detergents, pharmaceuticals, food and paper. In the present study, cellulase enzyme was partially purified from Bacillus pumilus ND8 strain isolated from garden waste and the pH and temperature values at which the enzyme showed optimum activity were determined. B. pumilus ND8 strain exhibited a cellulase activity of 8.6 UmL as a result of partial purification and the protein concentration of the enzyme was measured as 6.72 mgmL. The pH and temperature values at which the partially purified cellulase showed optimum activity were pH 5.5 and 60 C, respectively. Km and Vmax values of the enzyme were determined as 0.81 mM and 14.2 µmolmin, respectively. In conclusion, cellulase purified from B. pumilus ND8 strain was found to possess unique properties that make it suitable for industrial applications.

 Cellulose, one of the most abundant carbohydrates on Earth, is a promising candidate for the production of second-generation biofuels such as ethanol and various everyday products. This polysaccharide is degraded by the enzyme cellulase, which is usually produced by microorganisms. Microbial cellulases are widely used in various industries such as textiles, detergents, pharmaceuticals, food and paper. In the present study, cellulase enzyme was partially purified from Bacillus pumilus ND8 strain isolated from garden waste and the pH and temperature values at which the enzyme showed optimum activity were determined. B. pumilus ND8 strain exhibited a cellulase activity of 8.6 UmL as a result of partial purification and the protein concentration of the enzyme was measured as 6.72 mgmL. The pH and temperature values at which the partially purified cellulase showed optimum activity were pH 5.5 and 60 C, respectively. Km and Vmax values of the enzyme were determined as 0.81 mM and 14.2 µmolmin, respectively. In conclusion, cellulase purified from B. pumilus ND8 strain was found to possess unique properties that make it suitable for industrial applications.

 Cellulose, one of the most abundant carbohydrates on Earth, is a promising candidate for the production of second-generation biofuels such as ethanol and various everyday products. This polysaccharide is degraded by the enzyme cellulase, which is usually produced by microorganisms. Microbial cellulases are widely used in various industries such as textiles, detergents, pharmaceuticals, food and paper. In the present study, cellulase enzyme was partially purified from Bacillus pumilus ND8 strain isolated from garden waste and the pH and temperature values at which the enzyme showed optimum activity were determined. B. pumilus ND8 strain exhibited a cellulase activity of 8.6 UmL as a result of partial purification and the protein concentration of the enzyme was measured as 6.72 mgmL. The pH and temperature values at which the partially purified cellulase showed optimum activity were pH 5.5 and 60 C, respectively. Km and Vmax values of the enzyme were determined as 0.81 mM and 14.2 µmolmin, respectively. In conclusion, cellulase purified from B. pumilus ND8 strain was found to possess unique properties that make it suitable for industrial applications.

Makale detayları

Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste

Yazarlar:

Neslihan Dikbaş | Waleed Salih Salman Al Dahluz | Şeyma Alım | Sevda Uçar

Yayıncı:

Bingol University

Ana Başlıklar:

Enzimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.62-66

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328015

pISSN:

2149-6366

Bacillus pumilus

Cellulase

Partial purification

Sayı

Makale detayları

Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste

Yazarlar: Neslihan Dikbaş | Waleed Salih Salman Al Dahluz | Şeyma Alım | Sevda Uçar

Yayıncı: Bingol University

Ana Başlıklar: Enzimler

Dergi: Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.62-66

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328015

pISSN: 2149-6366

Bacillus pumilus

Cellulase

Partial purification

Sayı

Yazarlar:

Neslihan Dikbaş | Waleed Salih Salman Al Dahluz | Şeyma Alım | Sevda Uçar

Yayıncı:

Bingol University

Ana Başlıklar:

Enzimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.62-66

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328015

pISSN:

2149-6366