Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

Serdar ÜLKER; Arzu ÖZEL; Ahmet ÇOLAK; Şengül Alpay KARAOĞLU

Makale detayları

Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

Yazarlar:

Serdar ÜLKER | Arzu ÖZEL | Ahmet ÇOLAK | Şengül Alpay KARAOĞLU

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 5, pp.543-550

Belge Tipi:

Makale

Yayın Tarihi:

4/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812775

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and at 40 °C for 60 min. Ca2+ and Mn2+ enhanced lipase activity but it was determined that other metallic ions did not affect the enzyme activity. The Km and Vmax values of the crude enzyme for p-nitrophenyl butyrate hydrolysis were found to be 7.15 mM and 7.067 mM/min, respectively.

 

This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and at 40 °C for 60 min. Ca2+ and Mn2+ enhanced lipase activity but it was determined that other metallic ions did not affect the enzyme activity. The Km and Vmax values of the crude enzyme for p-nitrophenyl butyrate hydrolysis were found to be 7.15 mM and 7.067 mM/min, respectively.

Makale detayları

Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

Yazarlar:

Serdar ÜLKER | Arzu ÖZEL | Ahmet ÇOLAK | Şengül Alpay KARAOĞLU

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 5, pp.543-550

Belge Tipi:

Makale

Yayın Tarihi:

4/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812775

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Trichoderma harzianum

extracellular lipase

enzyme characterization

Sayı

Makale detayları

Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

Yazarlar: Serdar ÜLKER | Arzu ÖZEL | Ahmet ÇOLAK | Şengül Alpay KARAOĞLU

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2011 Vol.35 Issue 5, pp.543-550

Belge Tipi: Makale

Yayın Tarihi: 4/2011

Belge Dili: İngilizce

Document ID (Org No.): 2000812775

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Trichoderma harzianum

extracellular lipase

enzyme characterization

Sayı

Yazarlar:

Serdar ÜLKER | Arzu ÖZEL | Ahmet ÇOLAK | Şengül Alpay KARAOĞLU

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 5, pp.543-550

Belge Tipi:

Makale

Yayın Tarihi:

4/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812775

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152