Experimental study: effects of folic acid, vitamin C, and vitamin E on fat graft survival

Mehmet Fatih Okyay

Makale detayları

Experimental study: effects of folic acid, vitamin C, and vitamin E on fat graft survival

Yazarlar:

Mehmet Fatih Okyay

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Plastik | Rekonstrüktif ve Estetik Cerrahi

Dergi:

Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.561-565

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302598

pISSN:

2717-7505

Anahtar kelimeler:

Özet:

 Aim: This study explores the potential of folic acid vitamin B9, ascorbic acid vitamin C, and α-tocopherol vitamin E to enhance fat graft survival. Material and Method: The dorsal regions of the Sprague-Dawley rats n10 were divided into four quadrants as inguinal fat donor recipient sites. After incubation, fat grafts were placed into sites 0.9 NaCl-left cranial, vitamin B9-right cranial, vitamin C-left caudal, and vitamin E-right caudal. At the end of the third month, fat grafts were harvested. Fat grafts were evaluated immunohistochemically with perilipin to reveal viability. Results: Folic acid, vitamin C, and vitamin E were shown improved fat graft survival rate with perilipin p0.001, p0.001, p0.001, respectively. Conclusion: Effects of folic acid vitamin B9 in promoting angiogenesis and collagen synthesis, vitamin Cs antioxidant properties and involvement in collagen synthesis, vitamin Es potent antioxidant capacity, appeared to protect grafted adipose tissue from oxidative stress and inflammation, facilitates graft vascularization and integration, leading to enhanced survival. As an inexpensive and easy-to-supply and apply agents, vitamins B9, C, and E were found to improve fat graft survival immunohistochemically in our study. Increased graft viability by these medicines may inspire future clinical studies and practice.

 Aim: This study explores the potential of folic acid vitamin B9, ascorbic acid vitamin C, and α-tocopherol vitamin E to enhance fat graft survival. Material and Method: The dorsal regions of the Sprague-Dawley rats n10 were divided into four quadrants as inguinal fat donor recipient sites. After incubation, fat grafts were placed into sites 0.9 NaCl-left cranial, vitamin B9-right cranial, vitamin C-left caudal, and vitamin E-right caudal. At the end of the third month, fat grafts were harvested. Fat grafts were evaluated immunohistochemically with perilipin to reveal viability. Results: Folic acid, vitamin C, and vitamin E were shown improved fat graft survival rate with perilipin p0.001, p0.001, p0.001, respectively. Conclusion: Effects of folic acid vitamin B9 in promoting angiogenesis and collagen synthesis, vitamin Cs antioxidant properties and involvement in collagen synthesis, vitamin Es potent antioxidant capacity, appeared to protect grafted adipose tissue from oxidative stress and inflammation, facilitates graft vascularization and integration, leading to enhanced survival. As an inexpensive and easy-to-supply and apply agents, vitamins B9, C, and E were found to improve fat graft survival immunohistochemically in our study. Increased graft viability by these medicines may inspire future clinical studies and practice.

 Aim: This study explores the potential of folic acid vitamin B9, ascorbic acid vitamin C, and α-tocopherol vitamin E to enhance fat graft survival. Material and Method: The dorsal regions of the Sprague-Dawley rats n10 were divided into four quadrants as inguinal fat donor recipient sites. After incubation, fat grafts were placed into sites 0.9 NaCl-left cranial, vitamin B9-right cranial, vitamin C-left caudal, and vitamin E-right caudal. At the end of the third month, fat grafts were harvested. Fat grafts were evaluated immunohistochemically with perilipin to reveal viability. Results: Folic acid, vitamin C, and vitamin E were shown improved fat graft survival rate with perilipin p0.001, p0.001, p0.001, respectively. Conclusion: Effects of folic acid vitamin B9 in promoting angiogenesis and collagen synthesis, vitamin Cs antioxidant properties and involvement in collagen synthesis, vitamin Es potent antioxidant capacity, appeared to protect grafted adipose tissue from oxidative stress and inflammation, facilitates graft vascularization and integration, leading to enhanced survival. As an inexpensive and easy-to-supply and apply agents, vitamins B9, C, and E were found to improve fat graft survival immunohistochemically in our study. Increased graft viability by these medicines may inspire future clinical studies and practice.

 Öz Bu çalışma, folik asit vitamin B9, askorbik asit vitamin C ve α-tocopherol vitamin Enin yağ grefti sağ kalımını artırmadaki potansiyel etkilerini araştırmayı amaçlamaktadır. Gereç ve Yöntem Sprague-Dawley sıçanlarının n10 sırt bölgeleri kasık yağı donörü alıcı bölgeleri olarak dört kadrana bölündü. İnkübasyondan sonra yağ greftleri bölgelere 0,9 NaCl-sol kranyal, B9 vitamini-sağ kranyal, C vitamini-sol kaudal ve E vitamini-sağ kaudal yerleştirildi. Üçüncü ayın sonunda yağ greftleri toplandı. Yağ greftleri canlılığını ortaya çıkarmak için perilipin ile immünohistokimyasal olarak değerlendirildi. Sonuçlar Folik asit, C vitamini ve E vitamininin perilipin ile yağ grefti sağkalım oranını iyileştirdiği gösterilmiştir sırasıyla p0.001, p0.001, p0.001. Sonuç Folik asidin B9 vitamini anjiyogenezi ve kollajen sentezini teşvik etmedeki etkilerinin, C vitamininin antioksidan özelliklerinin ve kollajen sentezine katılımının, E vitamininin güçlü antioksidan kapasitesinin, greft olarak kullanılan yağ dokusunu oksidatif stres ve inflamasyondan koruduğu, greftin vaskülarizasyonunu ve entegrasyonunu kolaylaştırdığı gösterilmiş, sağ kalımın artmasına yol açtığı görülmüştür. Ucuz ve temini ve uygulaması kolay bir ajan olan B9, C ve E vitaminlerinin, çalışmamızda immünohistokimyasal olarak yağ grefti sağ kalımını arttırdığı bulunmuştur. Bu ilaçlarla artan greft canlılığı gelecekteki klinik çalışmalara ve uygulamalara ilham verebilir.

Makale detayları

Experimental study: effects of folic acid, vitamin C, and vitamin E on fat graft survival

Yazarlar:

Mehmet Fatih Okyay

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Plastik | Rekonstrüktif ve Estetik Cerrahi

Dergi:

Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.561-565

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302598

pISSN:

2717-7505

adipogenesis

adipogenesis

ascorbic acid

ascorbic acid

fat graft survival

fat graft survival

fat graft survival

fat graft survival

folic acid

folic acid

folic acid

folic acid

vitamin C

vitamin C

vitamin C

vitamin C

vitamin E

vitamin E

vitamin E

vitamin Eadipogenesis

ascorbic acid

fat graft survival

folic acid

vitamin C

vitamin E

Sayı

Makale detayları

Yazarlar: Mehmet Fatih Okyay

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Plastik | Rekonstrüktif ve Estetik Cerrahi

Dergi: Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.561-565

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302598

pISSN: 2717-7505

Sayı

Yazarlar:

Mehmet Fatih Okyay

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Plastik | Rekonstrüktif ve Estetik Cerrahi

Dergi:

Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.561-565

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302598

pISSN:

2717-7505