Molecular characterization of the encoding regions and tissue expression analyses for 3 novel buffalo AKT genes, AKT1, AKT2, and AKT3

Chunfeng WU; Lixian LIU; Jinlong HUO; Dalin LI; Yueyun YUAN; Feng YUAN; Yongwang MIAO

doi:https://doi.org/10.3906/biy-1305-33

Makale detayları

Molecular characterization of the encoding regions and tissue expression analyses for 3 novel buffalo AKT genes, AKT1, AKT2, and AKT3

Yazarlar:

Chunfeng WU | Lixian LIU | Jinlong HUO | Dalin LI | Yueyun YUAN | Feng YUAN | Yongwang MIAO

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 1, pp.63-75

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000812292

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1305-33

Anahtar kelimeler:

Özet:

 

The objective of this study was to obtain the complete coding sequences (CDSs) of 3 buffalo genes (AKT1, AKT2, and AKT3) using reverse-transcriptase polymerase chain reaction and to depict their molecular characterizations and tissue expression patterns in buffalo. The buffalo AKT1, AKT2, and AKT3 CDSs were 1443 bp, 1446 bp, and 1440 bp in length and encoded 480, 481, and 479 amino acids, respectively. Nine, 13, and 3 nucleotide differences were found in the CDSs between buffalo and other bovine species. Phylogenetic analyses showed that buffalo AKT1 and AKT2 have close genetic relationships with other species in the family Bovidae, while AKT3 is highly conserved in mammals. Buffalo AKT1, AKT2, and AKT3 all have a pleckstrin homology domain, a Ser/Thr-specific kinase domain, and a C-terminal hydrophobic regulatory domain. The tissue expression profiles were tested by real-time quantitative PCR and revealed that the buffalo AKTs were expressed in 10 tissues. Among these tissues, mammary glands showed high expression levels, which indicated that AKT genes might be important in the regulation of mammary gland functions in buffalo.

 

The objective of this study was to obtain the complete coding sequences (CDSs) of 3 buffalo genes (AKT1, AKT2, and AKT3) using reverse-transcriptase polymerase chain reaction and to depict their molecular characterizations and tissue expression patterns in buffalo. The buffalo AKT1, AKT2, and AKT3 CDSs were 1443 bp, 1446 bp, and 1440 bp in length and encoded 480, 481, and 479 amino acids, respectively. Nine, 13, and 3 nucleotide differences were found in the CDSs between buffalo and other bovine species. Phylogenetic analyses showed that buffalo AKT1 and AKT2 have close genetic relationships with other species in the family Bovidae, while AKT3 is highly conserved in mammals. Buffalo AKT1, AKT2, and AKT3 all have a pleckstrin homology domain, a Ser/Thr-specific kinase domain, and a C-terminal hydrophobic regulatory domain. The tissue expression profiles were tested by real-time quantitative PCR and revealed that the buffalo AKTs were expressed in 10 tissues. Among these tissues, mammary glands showed high expression levels, which indicated that AKT genes might be important in the regulation of mammary gland functions in buffalo.

Makale detayları

Molecular characterization of the encoding regions and tissue expression analyses for 3 novel buffalo AKT genes, AKT1, AKT2, and AKT3

Yazarlar:

Chunfeng WU | Lixian LIU | Jinlong HUO | Dalin LI | Yueyun YUAN | Feng YUAN | Yongwang MIAO

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 1, pp.63-75

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000812292

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1305-33

Key words: Buffalo

AKT genes

polymorphism

characterization

tissue expression profile

Sayı

Makale detayları

Molecular characterization of the encoding regions and tissue expression analyses for 3 novel buffalo AKT genes, AKT1, AKT2, and AKT3

Yazarlar: Chunfeng WU | Lixian LIU | Jinlong HUO | Dalin LI | Yueyun YUAN | Feng YUAN | Yongwang MIAO

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2014 Vol.38 Issue 1, pp.63-75

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: İngilizce

Document ID (Org No.): 2000812292

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1305-33

Key words: Buffalo

AKT genes

polymorphism

characterization

tissue expression profile

Sayı

Yazarlar:

Chunfeng WU | Lixian LIU | Jinlong HUO | Dalin LI | Yueyun YUAN | Feng YUAN | Yongwang MIAO

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 1, pp.63-75

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000812292

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1305-33