Investigation of the effects of Protocatechuic acid on apoptosis, oxidant and antioxidant status in Caco-2 colorectal cancer cells

Fatma Yıldız

Makale detayları

Investigation of the effects of Protocatechuic acid on apoptosis, oxidant and antioxidant status in Caco-2 colorectal cancer cells

Yazarlar:

Fatma Yıldız

Yayıncı:

Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Acta Medica Alanya Vol.8 Issue 2, pp.136-141

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326057

pISSN:

2587-0319

Anahtar kelimeler:

Özet:

 Aim: Protocatechuic acid PCA is one of the common phenolic acids found in many foods and plants and it has multiple biological activities. Although PCA has been investigated for its antioxidant, anti-inflammatory, and anticancer effects in various cell lines, its effects on molecules involved in the apoptotic pathway, especially in human colon cancer Caco-2 cells, have not been fully elucidated. This study aimed to investigate the effects of PCA on possible oxidant, antioxidant, and apoptosis mechanisms in Caco-2 cells, depending on dose and time. Methods: In the experimental study, 4 groups were created: control K, PCA 250-500-1000 µM. Total oxidant capacity TOC, total antioxidant capacity TAC, Oxidative stress index OSI, Bax, Bad, Bcl-2, Bcl-xl, and Caspase 9 protein levels were determined by the ELISA method in the cell lysates obtained from the groups. Results: The results showed that PCA treatment had apoptotic effects on Caco-2 cells at 24 and 48 h. PCA also decreased OSI levels by increasing TAC levels and decreasing TOC levels in a dose-dependent manner. Conclusion: As a result, it was determined that PCA has an apoptotic effect on the Caco-2 cell line and can be useful in the prevention andor treatment of colon cancer.

 Aim: Protocatechuic acid PCA is one of the common phenolic acids found in many foods and plants and it has multiple biological activities. Although PCA has been investigated for its antioxidant, anti-inflammatory, and anticancer effects in various cell lines, its effects on molecules involved in the apoptotic pathway, especially in human colon cancer Caco-2 cells, have not been fully elucidated. This study aimed to investigate the effects of PCA on possible oxidant, antioxidant, and apoptosis mechanisms in Caco-2 cells, depending on dose and time. Methods: In the experimental study, 4 groups were created: control K, PCA 250-500-1000 µM. Total oxidant capacity TOC, total antioxidant capacity TAC, Oxidative stress index OSI, Bax, Bad, Bcl-2, Bcl-xl, and Caspase 9 protein levels were determined by the ELISA method in the cell lysates obtained from the groups. Results: The results showed that PCA treatment had apoptotic effects on Caco-2 cells at 24 and 48 h. PCA also decreased OSI levels by increasing TAC levels and decreasing TOC levels in a dose-dependent manner. Conclusion: As a result, it was determined that PCA has an apoptotic effect on the Caco-2 cell line and can be useful in the prevention andor treatment of colon cancer.

 Aim: Protocatechuic acid PCA is one of the common phenolic acids found in many foods and plants and it has multiple biological activities. Although PCA has been investigated for its antioxidant, anti-inflammatory, and anticancer effects in various cell lines, its effects on molecules involved in the apoptotic pathway, especially in human colon cancer Caco-2 cells, have not been fully elucidated. This study aimed to investigate the effects of PCA on possible oxidant, antioxidant, and apoptosis mechanisms in Caco-2 cells, depending on dose and time. Methods: In the experimental study, 4 groups were created: control K, PCA 250-500-1000 µM. Total oxidant capacity TOC, total antioxidant capacity TAC, Oxidative stress index OSI, Bax, Bad, Bcl-2, Bcl-xl, and Caspase 9 protein levels were determined by the ELISA method in the cell lysates obtained from the groups. Results: The results showed that PCA treatment had apoptotic effects on Caco-2 cells at 24 and 48 h. PCA also decreased OSI levels by increasing TAC levels and decreasing TOC levels in a dose-dependent manner. Conclusion: As a result, it was determined that PCA has an apoptotic effect on the Caco-2 cell line and can be useful in the prevention andor treatment of colon cancer.

 Amaç: Protokatekuik asit PCA, birçok biyolojik aktiviteye sahip birçok gıda ve bitkide bulunan yaygın fenolik asitlerden biridir. PCAnın çeşitli hücre hatlarında antiinflamatuvar, antioksidan, antikanser etkileri araştırılmasına rağmen insan kolon kanseri Caco-2 hücrelerinde özellikle de apoptotik yolaklakta yer alan moleküller üzerindeki etkileri tam olarak aydınlatılamamıştır. Bu çalışmanın amacı PCAnın Caco-2 hücrelerinde doza ve zamana bağlı olarak olası oksidan, antioksidan ve apoptoz mekanizmaları üzerindeki etkilerini araştırmaktır. Yöntem: Deneysel çalışmada kontrol K, PCA 250-500-1000 µM olmak üzere 4 grup oluşturulmuştur. Gruplardan elde edilen hücre lizatlarında toplam oksidan kapasite TOC, toplam antioksidan kapasite TAC, Oksidatif stres indeksi OSI, Bax, Bad, Bcl-2, Bcl-xl, Kaspaz 9 protein seviyeleri ELISA yöntemiyle belirlenmiştir. Bulgular: Sonuçlar PCA tedavisinin Caco-2 hücrelerinde 24 ve 48 saatte apoptotik etkilere sahip olduğunu göstermiştir. Ayrıca PCA doza bağlı bir şekilde TAC seviyelerini artırırken TOC seviyelerini azaltarak OSI seviyelerini de azaltmıştır. Sonuç: Sonuç olarak PCAnın Caco-2 hücre hattında apoptotik etkiye sahip olduğu ve kolon kanserinin önlenmesinde veveya tedavisinde yararlı olabileceği belirlenmiştir.

Makale detayları

Investigation of the effects of Protocatechuic acid on apoptosis, oxidant and antioxidant status in Caco-2 colorectal cancer cells

Yazarlar:

Fatma Yıldız

Yayıncı:

Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Acta Medica Alanya Vol.8 Issue 2, pp.136-141

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326057

pISSN:

2587-0319

Colon cancer

Caco-2

Protocatechuic acid

Apoptosis

Oxidative stressKolon kanseri

Caco-2

Protokatekuik asit

Apoptoz

Oksidatif stres

Sayı

Makale detayları

Yazarlar: Fatma Yıldız

Yayıncı: Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar: Klinik Tıp Bilimleri (Diğer)

Dergi: Acta Medica Alanya Vol.8 Issue 2, pp.136-141

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326057

pISSN: 2587-0319

Sayı

Yazarlar:

Fatma Yıldız

Yayıncı:

Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Acta Medica Alanya Vol.8 Issue 2, pp.136-141

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326057

pISSN:

2587-0319