The Combined Effects of Etanercept Plus Methylprednisolone on Pancreatic Oxidative Stress in Acute Pancreatitis Induced by Cerulein

Murat GÜNAY; Erol KILIÇ; Ramazan AMANVERMEZ

Makale detayları

The Combined Effects of Etanercept Plus Methylprednisolone on Pancreatic Oxidative Stress in Acute Pancreatitis Induced by Cerulein

Yazarlar:

Murat GÜNAY | Erol KILIÇ | Ramazan AMANVERMEZ

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Experimental and Clinical Medicine 2011 Vol.28 Issue 1, pp.12-16

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000509618

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483

Özet:

 

ABSTRACT
Whether etanercept plus metylprednisolone reduce pancreatic oxidative stress and injury during pancreatitis is unknown well. The aim of this study was to examine the therapeutic effects of etanercept and methylprednisolone (MP) on acute pancreatitis and oxidative stress in acute pancreatitis (AP) induced by cerulein in a rat model. The study was performed with 48 rats divided into 6 groups (n = 8/group): 1-sham, 2-cerulein-induced pancreatitis (over 10 hours), 3- cerulein-pancreatitis (over 20 hours), 4-etanercept (5 mg/kg, i.p.), 5-methylprednisolone (10 mg/kg, i.m.), 6-etanercept plus methylprednisolone. Also, the rats in groups 4, 5, and 6 were cerulein-induced pancreatitis at 20 hours. After the treatment, the pancreas and blood were taken for histopathological and biochemical analysis. All cerulein-treated rats developed biochemical and pathological acute pancreatitis after 10-20 hours. The markers of oxidative stress such as protein carbonyls, lipid peroxidation, and myeloperoxidase (mpo) in the pancreas tissues were increased in the group 2 and 3, but these were lower in etanercept and MP-treated rats compared to groups 3. Pancreatic mpo activity was considerably reduced in pancreas tissues at 20 h after the administration of etanercept plus metylprednisolone in the group 6 compared to group 3. In AP induced by cerulein, the treatment of etanercept plus methylprednisolone may ameliorate pancreatic oxidative stress in rats. .

Makale detayları

The Combined Effects of Etanercept Plus Methylprednisolone on Pancreatic Oxidative Stress in Acute Pancreatitis Induced by Cerulein

Yazarlar: Murat GÜNAY | Erol KILIÇ | Ramazan AMANVERMEZ

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Journal of Experimental and Clinical Medicine 2011 Vol.28 Issue 1, pp.12-16

Belge Tipi: Makale

Yayın Tarihi: 2/2012

Belge Dili: İngilizce

Document ID (Org No.): 2000509618

Sıklık: Üç Aylık

eISSN: 1309-5129

pISSN: 1309-4483

Sayı

Yazarlar:

Murat GÜNAY | Erol KILIÇ | Ramazan AMANVERMEZ

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Experimental and Clinical Medicine 2011 Vol.28 Issue 1, pp.12-16

Belge Tipi:

Makale

Yayın Tarihi:

2/2012

Belge Dili:

İngilizce

Document ID (Org No.):

2000509618

Sıklık:

Üç Aylık

eISSN:

1309-5129

pISSN:

1309-4483