Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe

Kamil IŞIK; Nevzat ŞAHİN; Ergin KARİPTAŞ

Makale detayları

Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe

Yazarlar:

Kamil IŞIK | Nevzat ŞAHİN | Ergin KARİPTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

12/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813751

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from 1.2 to 20.7 kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically well-established species, except N. pseudobrasiliensis. Therefore, it can be concluded that ribotyping technique provide a useful information for the classification of clinically significant Nocardia.

 

In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from 1.2 to 20.7 kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically well-established species, except N. pseudobrasiliensis. Therefore, it can be concluded that ribotyping technique provide a useful information for the classification of clinically significant Nocardia.

Makale detayları

Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe

Yazarlar:

Kamil IŞIK | Nevzat ŞAHİN | Ergin KARİPTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

12/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813751

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Nocardia

Ribotyping

Taxonomy

Sayı

Makale detayları

Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe

Yazarlar: Kamil IŞIK | Nevzat ŞAHİN | Ergin KARİPTAŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.1-8

Belge Tipi: Makale

Yayın Tarihi: 12/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000813751

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Nocardia

Ribotyping

Taxonomy

Sayı

Yazarlar:

Kamil IŞIK | Nevzat ŞAHİN | Ergin KARİPTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

12/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813751

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152