Postnatal expression profiles of phospholipid hydroperoxide glutathioneperoxidase in spermatogenesis in mice

Ki Youn JUNG; Chunmei LIN; Jung-min YON; A Young JUNG; Seul Gi PARK; Sl Bi CHU; Lee Wha GWON; Sang Yoon NAM

Makale detayları

Postnatal expression profiles of phospholipid hydroperoxide glutathioneperoxidase in spermatogenesis in mice

Yazarlar:

Ki Youn JUNG | Chunmei LIN | Jung-min YON | A Young JUNG | Seul Gi PARK | Sl Bi CHU | Lee Wha GWON | Sang Yoon NAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2016 Vol.40 Issue 1, pp.40-46

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000765289

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is known to be an antioxidant enzyme that may be implicated in spermatogenesis, but its functional significance in the development of testes remains poorly understood. To demonstrate the roles of PHGPx in spermatogenesis during postnatal development, expression profiles of PHGPx mRNA and protein in testes and epididymides obtained from mice 1-9 and 27 weeks after birth were spatiotemporally analyzed. In testes, PHGPx mRNA first appeared in 2-week-old mice, while its protein was first detected in late spermatocytes and round spermatids from 3-week-old mice and levels were greatly increased in elongated spermatids and immature spermatozoa of 5-week-old mice. These increased levels were maintained until 27 weeks as well as weak expression in Leydig cells. In the epididymides, PHGPx mRNA was weakly detected in all ages examined, while PHGPx protein first appeared at 4 weeks, greatly increased after 6 weeks, and was observed strongly in luminal sperm and weakly in surrounding tubular epithelium until 27 weeks. These findings indicate that PHGPx is essential for postnatal spermatogenic activity in mice.

Makale detayları

Postnatal expression profiles of phospholipid hydroperoxide glutathioneperoxidase in spermatogenesis in mice

Yazarlar: Ki Youn JUNG | Chunmei LIN | Jung-min YON | A Young JUNG | Seul Gi PARK | Sl Bi CHU | Lee Wha GWON | Sang Yoon NAM

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2016 Vol.40 Issue 1, pp.40-46

Belge Tipi: Makale

Yayın Tarihi: 1/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000765289

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Ki Youn JUNG | Chunmei LIN | Jung-min YON | A Young JUNG | Seul Gi PARK | Sl Bi CHU | Lee Wha GWON | Sang Yoon NAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2016 Vol.40 Issue 1, pp.40-46

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000765289

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128