Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model

Nurhan Akaras; Erdem Toktay; Hasan Şimşek; Nevra Aydemir Celep; Neslihan Yüce

Makale detayları

Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model

Yazarlar:

Nurhan Akaras | Erdem Toktay | Hasan Şimşek | Nevra Aydemir Celep | Neslihan Yüce

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Histoloji ve Embriyolojisi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.252-259

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293974

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 Sepsis is an inflammatory syndrome that targets the lung tissue. Bromelain BRO is an enzyme complex known for its anti-inflammatory properties. In this study, the protective effect of BRO on lung injury due to sepsis induced by cecal ligation and puncture CLP in Sprague-Dawley rats was investigated by biochemical and histopathological methods. Forty Sprague dawley rats were randomly divided into five groups as control, CLP, BRO, CLPBRO 50 and CLPBRO 100. In the study, after oral administration of 50 and 100 mgkg BRO total of 7 applications for 7 days to rats, laparotomy and CLP were performed. The data showed that due to its antioxidant properties, BRO decreased CLP-induced myeloperoxidase MPO and lipid peroxidation MDA p 0,001, increased glutathione GSH levels, superoxide dismutase SOD and glutathione peroxidase GPx activities p 0,001. Additionally, CLP-induced lung histological changes were reduced with BRO supplementation. The results showed that BRO treatment has an antioxidant, anti-inflammatory effect on CLP-induced lung toxicity and helps preserve lung tissue architecture owing to its regulatory effect.

 Sepsis is an inflammatory syndrome that targets the lung tissue. Bromelain BRO is an enzyme complex known for its anti-inflammatory properties. In this study, the protective effect of BRO on lung injury due to sepsis induced by cecal ligation and puncture CLP in Sprague-Dawley rats was investigated by biochemical and histopathological methods. Forty Sprague dawley rats were randomly divided into five groups as control, CLP, BRO, CLPBRO 50 and CLPBRO 100. In the study, after oral administration of 50 and 100 mgkg BRO total of 7 applications for 7 days to rats, laparotomy and CLP were performed. The data showed that due to its antioxidant properties, BRO decreased CLP-induced myeloperoxidase MPO and lipid peroxidation MDA p 0,001, increased glutathione GSH levels, superoxide dismutase SOD and glutathione peroxidase GPx activities p 0,001. Additionally, CLP-induced lung histological changes were reduced with BRO supplementation. The results showed that BRO treatment has an antioxidant, anti-inflammatory effect on CLP-induced lung toxicity and helps preserve lung tissue architecture owing to its regulatory effect.

 Sepsis is an inflammatory syndrome that targets the lung tissue. Bromelain BRO is an enzyme complex known for its anti-inflammatory properties. In this study, the protective effect of BRO on lung injury due to sepsis induced by cecal ligation and puncture CLP in Sprague-Dawley rats was investigated by biochemical and histopathological methods. Forty Sprague dawley rats were randomly divided into five groups as control, CLP, BRO, CLPBRO 50 and CLPBRO 100. In the study, after oral administration of 50 and 100 mgkg BRO total of 7 applications for 7 days to rats, laparotomy and CLP were performed. The data showed that due to its antioxidant properties, BRO decreased CLP-induced myeloperoxidase MPO and lipid peroxidation MDA p 0,001, increased glutathione GSH levels, superoxide dismutase SOD and glutathione peroxidase GPx activities p 0,001. Additionally, CLP-induced lung histological changes were reduced with BRO supplementation. The results showed that BRO treatment has an antioxidant, anti-inflammatory effect on CLP-induced lung toxicity and helps preserve lung tissue architecture owing to its regulatory effect.

 Sepsis, inflamatuvar bir sendrom olup başta akciğeri hedef almaktadır. Bromelainin BRO anti-inflamatuvar özelliği ile bilinen enzim kompleksidir. Bu çalışmada Sprague dawley sıçanlarında çekal ligasyon ve perforasyon CLP ile indüklenen sepsise bağlı akciğer hasarında BRO koruyucu etkisi biyokimyasal ve histopatolojik yöntemlerle araştırıldı. Kırk adet Sprague dawley sıçan, kontrol, CLP, BRO, CLPBRO 50 ve CLPBRO 100 olmak üzere rastgele beş gruba ayrıldı. Çalışmada sıçanlara 50 ve 100 mgkg BRO oral olarak 7 gün boyunca toplam 7 uygulama verildikten sonra laparotomi ve CLP uygulanmıştır. Veriler, BROnun antioksidan özelliklerinden dolayı CLP ile indüklenen miyeloperoksidaz MPO ve lipid peroksidasyonu MDA azalttığını p 0,001, glutatyon GSH seviyelerini, süperoksit dismutaz SOD ve glutatyon peroksidaz GPx aktivitelerini arttırdığını göstermiştir p 0,001. Ayrıca CLP kaynaklı akciğer histolojik değişiklikler BRO takviyesi ile azalmıştır. Sonuçlar BRO tedavisin CLP kaynaklı akciğer toksisitesi üzerinde antioksidan, anti-inflamatuvar etkiye sahip olduğunu ve düzenleyici etkisi sayesinde akciğer doku mimarisinin korunmasına yardımcı olduğunu göstermiştir.

Makale detayları

Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model

Yazarlar:

Nurhan Akaras | Erdem Toktay | Hasan Şimşek | Nevra Aydemir Celep | Neslihan Yüce

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Histoloji ve Embriyolojisi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.252-259

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293974

pISSN:

1308-1594

Lung

Bromelain

Sepsis

Inflammation

Oxidative StressAkciğer

Bromelain

Sepsis

İnflamasyon

Oksidatif Stres

Sayı

Makale detayları

Yazarlar: Nurhan Akaras | Erdem Toktay | Hasan Şimşek | Nevra Aydemir Celep | Neslihan Yüce

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Histoloji ve Embriyolojisi

Dergi: Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.252-259

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293974

pISSN: 1308-1594

Sayı

Yazarlar:

Nurhan Akaras | Erdem Toktay | Hasan Şimşek | Nevra Aydemir Celep | Neslihan Yüce

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Histoloji ve Embriyolojisi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.252-259

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293974

pISSN:

1308-1594