The comparing of homogenization methods for oxidative and antioxidative analyses of sperm

Ömer VARIŞLI; Numan AKYOL

Makale detayları

The comparing of homogenization methods for oxidative and antioxidative analyses of sperm

Yazarlar:

Ömer VARIŞLI | Numan AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2019 Vol.43 Issue 6, pp.852-856

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000764125

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differences can affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semen of four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods. Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, free sulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods before oxidative and antioxidative status analyses significantly affected the result (P < 0.05), and the ultrasonic homogenization method was the most effective. It is recommended to use the ultrasonic homogenization as a pretreatment method to determine the oxidative and antioxidative status analyses in sperm.

Makale detayları

The comparing of homogenization methods for oxidative and antioxidative analyses of sperm

Yazarlar: Ömer VARIŞLI | Numan AKYOL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2019 Vol.43 Issue 6, pp.852-856

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000764125

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Ömer VARIŞLI | Numan AKYOL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2019 Vol.43 Issue 6, pp.852-856

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000764125

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128