INVESTIGATION OF SFLT-1 AND VEGF EXPRESSION IN NORMOTENSIVE AND PREECLAMPTIC PLACENTA.  AN IMMUNOHISTOCHEMICAL STUDY

Firat SAHİN; Murat AKKUŞ; Uğur ŞEKER; Sevda SOKER; Ebru GOKALP-OZKORKMAZ; Elif AĞAÇAYAK; Fırat AŞIR

doi:https://doi.org/10.33457/ijhsrp.766837

Makale detayları

INVESTIGATION OF SFLT-1 AND VEGF EXPRESSION IN NORMOTENSIVE AND PREECLAMPTIC PLACENTA. AN IMMUNOHISTOCHEMICAL STUDY

Yazarlar:

Firat SAHİN | Murat AKKUŞ | Uğur ŞEKER | Sevda SOKER | Ebru GOKALP-OZKORKMAZ | Elif AĞAÇAYAK | Fırat AŞIR

Yayıncı:

Rojan GÜMÜŞ

Ana Başlıklar:

Hastalıklar

Dergi:

International Journal of Health Services Research and Policy 2020 Vol.5 Issue 3, pp.206-214

Belge Tipi:

Makale

Yayın Tarihi:

12/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000465136

Sıklık:

Üç Yıllık

eISSN:

2602-3482

DOI:

https://doi.org/10.33457/ijhsrp.766837

Özet:

 

Objectives: The pathogenesis of preeclampsia is still not clear, but endothelial dysfunction believed to be one of the most encountered problems during placenta development in preeclamptic patients. Both vascular endothelial growth factor (VEGF) and its antagonist, soluble Fms-Like tyrosine kinase-1 (sFlt-1), have roles in vascular function. In this study, we have investigated immunohistochemical expression of VEGF and sFlt-1 in term placenta of normotensive and preeclampsia patients.
Methods: Totally twenty term placentas were obtained from pregnant women of which 10 preeclampsia patient and 10 normotensive. Placentas were dissected and tissue samples were subjected to routine tissue processing protocol, then embedded in paraffin blocks. Serial sections were obtained from paraffin blocks and stained with H&E and PAS for routine histopathology. VEGF and sFlt-1 immunohistochemistry was performed to the sections.
Results: When compared to control group, severe pathological changes were observed in preeclamptic placentas. Increase in number of syncytial knots and intervillous bridges, hemorrhage in interstitium, dilatation and congestion in villous capillaries, increase in fibrin accumulation in villus stroma, and increase in thickening of basement membrane were very clear. VEGF expression was significantly higher in normotensive placentas compared to preeclamptic. On the other hand, sFlt-1 expression was significantly increased in preeclamptic placentas.
Conclusions: When the VEGF and sFlt-1 expression was considered, higher expression of sFlt-1 at preeclampsia, but decrease in VEGF expression, might be related to endothelial dysfunction in preeclampsia. Overall, this study demonstrates, imbalance between VEGF and sFlt-1 is one of the major reason of endothelial dysfunction in preeclamptic placenta.

Makale detayları

INVESTIGATION OF SFLT-1 AND VEGF EXPRESSION IN NORMOTENSIVE AND PREECLAMPTIC PLACENTA. AN IMMUNOHISTOCHEMICAL STUDY

Yazarlar: Firat SAHİN | Murat AKKUŞ | Uğur ŞEKER | Sevda SOKER | Ebru GOKALP-OZKORKMAZ | Elif AĞAÇAYAK | Fırat AŞIR

Yayıncı: Rojan GÜMÜŞ

Ana Başlıklar: Hastalıklar

Dergi: International Journal of Health Services Research and Policy 2020 Vol.5 Issue 3, pp.206-214

Belge Tipi: Makale

Yayın Tarihi: 12/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000465136

Sıklık: Üç Yıllık

eISSN: 2602-3482

DOI: https://doi.org/10.33457/ijhsrp.766837

Sayı

Yazarlar:

Firat SAHİN | Murat AKKUŞ | Uğur ŞEKER | Sevda SOKER | Ebru GOKALP-OZKORKMAZ | Elif AĞAÇAYAK | Fırat AŞIR

Yayıncı:

Rojan GÜMÜŞ

Ana Başlıklar:

Hastalıklar

Dergi:

International Journal of Health Services Research and Policy 2020 Vol.5 Issue 3, pp.206-214

Belge Tipi:

Makale

Yayın Tarihi:

12/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000465136

Sıklık:

Üç Yıllık

eISSN:

2602-3482

DOI:

https://doi.org/10.33457/ijhsrp.766837