99mTc labeled levofloxacin as an infection imaging agent: a novel method for labeling levofloxacin using cysteine\cdot HCl as co-ligand and in vivo study

Syed Ali Raza NAQVI; Malik Muhammad ISHFAQ; Zulfiqar Ali KHAN

Makale detayları

99mTc labeled levofloxacin as an infection imaging agent: a novel method for labeling levofloxacin using cysteine\cdot HCl as co-ligand and in vivo study

Yazarlar:

Syed Ali Raza NAQVI | Malik Muhammad ISHFAQ | Zulfiqar Ali KHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2012 Vol.36 Issue 2, pp.267-277

Belge Tipi:

Makale

Yayın Tarihi:

1/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000727653

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Levofloxacin was labeled with 99mTc using cysteine\cdot  HCl as co-ligand and SnCl2.2H2O as reducing agent.  The influence of  various parameters such as amount of cysteine\cdot HCl, reducing  agent, pH value, and reaction time on labeling process was studied.  After optimizing the conditions the labeling was performed at pH 5  using 1 mg of levofloxacin, 500 m g of cysteine\cdot HCl, 50 m g  of SnCl2.2H2O, and 15 min reaction time.  The radiochemical purity  was determined with the help of instant thin layer chromatography  (ITLC) and reverse phase high performance liquid chromatography  (RP-HPLC) which was more than 95% and was stable for up to 6 h.  Biodistribution of 99mTc-levofloxacin (99mTc-lefx) was studied  in infection induced rat models using live Staphylococcus aureus and  heat killed S. aureus (sterile inflammation model).  In the case of  the live S. aureus induced abscess model, the accumulation of  99mTc-lefx at target was 3.96, which was higher than that of  99mTc-ciprofloxacin (99mTc-cifx), taken as the control.

 

Levofloxacin was labeled with 99mTc using cysteine\cdot  HCl as co-ligand and SnCl2.2H2O as reducing agent.  The influence of  various parameters such as amount of cysteine\cdot HCl, reducing  agent, pH value, and reaction time on labeling process was studied.  After optimizing the conditions the labeling was performed at pH 5  using 1 mg of levofloxacin, 500 m g of cysteine\cdot HCl, 50 m g  of SnCl2.2H2O, and 15 min reaction time.  The radiochemical purity  was determined with the help of instant thin layer chromatography  (ITLC) and reverse phase high performance liquid chromatography  (RP-HPLC) which was more than 95% and was stable for up to 6 h.  Biodistribution of 99mTc-levofloxacin (99mTc-lefx) was studied  in infection induced rat models using live Staphylococcus aureus and  heat killed S. aureus (sterile inflammation model).  In the case of  the live S. aureus induced abscess model, the accumulation of  99mTc-lefx at target was 3.96, which was higher than that of  99mTc-ciprofloxacin (99mTc-cifx), taken as the control.

Makale detayları

99mTc labeled levofloxacin as an infection imaging agent: a novel method for labeling levofloxacin using cysteine\cdot HCl as co-ligand and in vivo study

Yazarlar: Syed Ali Raza NAQVI | Malik Muhammad ISHFAQ | Zulfiqar Ali KHAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2012 Vol.36 Issue 2, pp.267-277

Belge Tipi: Makale

Yayın Tarihi: 1/2012

Belge Dili: Türkçe

Document ID (Org No.): 2000727653

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Syed Ali Raza NAQVI | Malik Muhammad ISHFAQ | Zulfiqar Ali KHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2012 Vol.36 Issue 2, pp.267-277

Belge Tipi:

Makale

Yayın Tarihi:

1/2012

Belge Dili:

Türkçe

Document ID (Org No.):

2000727653

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527