Tigecycline Resistance among Clinical Isolates of Staphylococcus aureus from North-east India

Swati Sharma; Deepshikha Bhowmik; Amitabha Bhattacharjee

doi:https://doi.org/10.5799/jmid.368660

Makale detayları

Tigecycline Resistance among Clinical Isolates of Staphylococcus aureus from North-east India

Yazarlar:

Swati Sharma | Deepshikha Bhowmik | Amitabha Bhattacharjee

Yayıncı:

Sağlık Araştırmaları Derneği

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Microbiology and Infectious Diseases 2017 Vol.07 Issue 04, pp.173-177

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000487440

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/jmid.368660

Özet:

 


Objective: The current study investigated the resistance against
cycline group of antibiotic among clinical isolates of Staphylococcus aureus and molecular characterization with special
reference to phenotypic screening of Tigecycline resistance.


Methods:
197 coagulase producing Staphylococcus spp. were collected from private
diagnostic laboratories and Silchar Medical College and Hospital, India.
Phenotypic screening was done with cycline groups of antimicrobials and
genotypically characterized by PCR, targeting coa gene. Detection of tetK,
tetM, tetO and tetX genes was
performed by PCR. Antibiogram profiling was performed and clonality was also
determined. 


Results:
127 coagulase positive S. aureus were
phenotypically screened with cycline group of antibiotics in which prominent
resistant type showed highest non-susceptibility against Tigecycline 53.5%
(n=68) while lowest against minocycline 20.5% (n=26). Multiplex PCR assay
showed that tet(K) gene to be the
most common cycline group of antimicrobial resistance determinant in S. aureus, followed by tet(M) gene. Multiple genes were also
observed. However, resistant gene tet(O)
was not reported. Antibiotic susceptibility testing and MIC for therapeutic
purpose showed highest susceptibility rate against linezolid. DNA
fingerprinting experimented with PFGE had shown seven pulsotypes.


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Makale detayları

Tigecycline Resistance among Clinical Isolates of Staphylococcus aureus from North-east India

Yazarlar: Swati Sharma | Deepshikha Bhowmik | Amitabha Bhattacharjee

Yayıncı: Sağlık Araştırmaları Derneği

Ana Başlıklar: Sağlık teşvikleri

Dergi: Journal of Microbiology and Infectious Diseases 2017 Vol.07 Issue 04, pp.173-177

Belge Tipi: Makale

Yayın Tarihi: 12/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000487440

Sıklık: Üç Aylık

eISSN: 2146-9369

pISSN: 2146-3158

DOI: https://doi.org/10.5799/jmid.368660

Sayı

Yazarlar:

Swati Sharma | Deepshikha Bhowmik | Amitabha Bhattacharjee

Yayıncı:

Sağlık Araştırmaları Derneği

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Journal of Microbiology and Infectious Diseases 2017 Vol.07 Issue 04, pp.173-177

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000487440

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/jmid.368660