Molecular Characterization of the Gene Encoding for the Salivaricin B Activity and its Flanking Sequences

Osman ÇATALOLUK

Makale detayları

Molecular Characterization of the Gene Encoding for the Salivaricin B Activity and its Flanking Sequences

Yazarlar:

Osman ÇATALOLUK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 4, pp.379-386

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813777

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. Since they are associated with foods of plant and animal origins, the shared interest is that they can be utilized as a vector to deliver the active constituents at the target in the gastrointestinal tract after digestion and be used in therapy. In this respect, the molecular pattern of the expression and translocation of salivaricin B (SalB), a bacteriocin, from Lactobacillus salivarius M7 was studied at the molecular level. The gene encoding SalB and the flanking sequences were obtained and sequenced. The gene encoding SalB comprised an open reading frame (ORF) of 171 bp having a 57 bp long leader sequence and a 114 bp long structural part. Ribosomal binding site (GAGG, RBS) is located at a cannonical distance of 8 bp upstream from the start site.

 

Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. Since they are associated with foods of plant and animal origins, the shared interest is that they can be utilized as a vector to deliver the active constituents at the target in the gastrointestinal tract after digestion and be used in therapy. In this respect, the molecular pattern of the expression and translocation of salivaricin B (SalB), a bacteriocin, from Lactobacillus salivarius M7 was studied at the molecular level. The gene encoding SalB and the flanking sequences were obtained and sequenced. The gene encoding SalB comprised an open reading frame (ORF) of 171 bp having a 57 bp long leader sequence and a 114 bp long structural part. Ribosomal binding site (GAGG, RBS) is located at a cannonical distance of 8 bp upstream from the start site.

Makale detayları

Molecular Characterization of the Gene Encoding for the Salivaricin B Activity and its Flanking Sequences

Yazarlar:

Osman ÇATALOLUK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 4, pp.379-386

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813777

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Gene

Gene structure

Salivaricin B

PCR

Inverse PCR

Degenerate primers

Lactobacillus salivarius M7.

Sayı

Makale detayları

Yazarlar: Osman ÇATALOLUK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2001 Vol.25 Issue 4, pp.379-386

Belge Tipi: Makale

Yayın Tarihi: 3/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000813777

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Osman ÇATALOLUK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2001 Vol.25 Issue 4, pp.379-386

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813777

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152