Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

Dilek ÖZBEYLİ; Ali ŞEN; Aslı AYKAÇ; Kerem TERALI; Özlem Tuğçe ÇİLİNGİR-KAYA; İsmail ŞENKARDEŞ; Göksel ŞENER

doi:https://doi.org/10.26650/EurJBiol.2021.1016222

Makale detayları

Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

Yazarlar:

Dilek ÖZBEYLİ | Ali ŞEN | Aslı AYKAÇ | Kerem TERALI | Özlem Tuğçe ÇİLİNGİR-KAYA | İsmail ŞENKARDEŞ | Göksel ŞENER

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2021 Vol.80 Issue 2, pp.119-128

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000424333

eISSN:

2618-6144

pISSN:

2602-2575

DOI:

https://doi.org/10.26650/EurJBiol.2021.1016222

Özet:

 


Objective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. 

Materials and Methods: The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), and sulfasalazine (SS)-treated colitis (AA+SS) groups. Colitis was induced by acetic acid. MoC extract, SS or saline were given to the related groups for 3 days. Interleukine-1β, malondialdehyde, glutathione levels, myeloperoxidase activity, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were measured in colon and macroscopic and histopathologic examinations were done. Total phenolic/flavonoid content and biological activity of MoC were evaluated by in vitro analysis. 

Results: Compared to the control group, with acetic acid application interleukin-1β levels, myeloperoxidase activity, malondialdehyde levels, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were significantly upregulated, while glutathione levels were significantly decreased in the AA group. In contrast, MoC and SS treatments reduced interleukin-1β, malondialdehyde levels, myeloperoxidase activity, bax/bcl-2 ratio, and caspase-9 and caspase-3 levels. Glutathione levels increased upon MoC or SS treatment. Increased macroscopic and microscopic scoring with AA improved with MoC or SS treatment, but the MoC or SS treated groups had higher score values than the control. Also, in vitro results showed that MoC exhibited 2,2-diphenyl-1- picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity as well as significant antilipoxygenase activity. In addition, MoC extract showed a potent anti-inflammatory activity compared to standard indomethacin. 

Conclusion: Our biochemical, in vitro and histopathologic analysis indicate that MoC is likely to prove beneficial in UC therapy.

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Makale detayları

Therapeutic Effects of Momordica charantia L. Ethanolic Extract on Acetic Acid-Induced Ulcerative Colitis in Rats

Yazarlar: Dilek ÖZBEYLİ | Ali ŞEN | Aslı AYKAÇ | Kerem TERALI | Özlem Tuğçe ÇİLİNGİR-KAYA | İsmail ŞENKARDEŞ | Göksel ŞENER

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2021 Vol.80 Issue 2, pp.119-128

Belge Tipi: Makale

Yayın Tarihi: 12/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000424333

eISSN: 2618-6144

pISSN: 2602-2575

DOI: https://doi.org/10.26650/EurJBiol.2021.1016222

Sayı

Yazarlar:

Dilek ÖZBEYLİ | Ali ŞEN | Aslı AYKAÇ | Kerem TERALI | Özlem Tuğçe ÇİLİNGİR-KAYA | İsmail ŞENKARDEŞ | Göksel ŞENER

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2021 Vol.80 Issue 2, pp.119-128

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000424333

eISSN:

2618-6144

pISSN:

2602-2575

DOI:

https://doi.org/10.26650/EurJBiol.2021.1016222