Characterization of intracellular β-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activity

Şaban TUNÇ; Fatma MATPAN BEKLER; Kemal GÜVEN

doi:https://doi.org/10.38042/biotechstudies.953514

Makale detayları

Characterization of intracellular β-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activity

Yazarlar:

Şaban TUNÇ | Fatma MATPAN BEKLER | Kemal GÜVEN

Yayıncı:

Tarla Bitkileri Merkez Araştırma Enstitüsü

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Biotech Studies 2021 Vol.30 Issue 2, pp.71-78

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000904891

Sıklık:

Aylık

eISSN:

2757-5233

pISSN:

2687-3761

DOI:

https://doi.org/10.38042/biotechstudies.953514

Özet:

 

In this study, the intracellular β-galactosidases of Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from Bingöl Binkap hot spring was partially purified and characterized. As a result of purification, the yield of the enzyme for B. subtilis 4NK was 85.2% and the purification fold was 2.8. The yield for B. paralicheniformis 5NK was 76.8% and the purification fold was 2.0. The optimum temperature of the enzyme was determined as 45 oC for B. subtilis 4NK and 55 oC for B. paralicheniformis 5NK and the optimum pH was 6.0 for both. In addition, in the thermal stability experiments even at the end of 120 min both enzymes were stable at 50 oC. It was determined that the partially purified enzyme activity increased in the presence of iodoacetamide and phenylmethylsulfonylfluoride for B. subtilis 4NK, dithiothreitol, N-ethylenemaleimide and phenylmethylsulfonylfluoride for B. paralicheniformis 5NK. The metals were found to activate the enzyme at low concentrations of Co2+, Cd2+ and Mn2+ for B. subtilis 4NK, Cu2+ and Cd2+ were found to inhibit the enzyme at high rates for B. paralicheniformis 5NK. Km and Vmax values for 4NK and 5NK, respectively; 23.80 mM, 1.978 μmol/min and 5.61 mM, 1.869 μmol/min.

Makale detayları

Characterization of intracellular β-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activity

Yazarlar: Şaban TUNÇ | Fatma MATPAN BEKLER | Kemal GÜVEN

Yayıncı: Tarla Bitkileri Merkez Araştırma Enstitüsü

Ana Başlıklar: Yaşam bilimleri

Dergi: Biotech Studies 2021 Vol.30 Issue 2, pp.71-78

Belge Tipi: Makale

Yayın Tarihi: 12/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000904891

Sıklık: Aylık

eISSN: 2757-5233

pISSN: 2687-3761

DOI: https://doi.org/10.38042/biotechstudies.953514

Sayı

Yazarlar:

Şaban TUNÇ | Fatma MATPAN BEKLER | Kemal GÜVEN

Yayıncı:

Tarla Bitkileri Merkez Araştırma Enstitüsü

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Biotech Studies 2021 Vol.30 Issue 2, pp.71-78

Belge Tipi:

Makale

Yayın Tarihi:

12/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000904891

Sıklık:

Aylık

eISSN:

2757-5233

pISSN:

2687-3761

DOI:

https://doi.org/10.38042/biotechstudies.953514