Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells

Tuğba Alarcon MARTİNEZ; Naciye Dilara ZEYBEK; Sevda MÜFTÜOĞLU

Makale detayları

Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells

Yazarlar:

Tuğba Alarcon MARTİNEZ | Naciye Dilara ZEYBEK | Sevda MÜFTÜOĞLU

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2018 Vol.35 Issue 3, pp.256-262

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000758790

Sıklık:

İki Aylık

pISSN:

2146-3123

Özet:

 

Background: Recently, cytotoxic effects of statins on breast
 cancer cells have been reported. However, the mechanism of antiproliferative
 effects is currently unknown. Autophagy is non-apoptotic
 programmed cell death, which is characterized by degradation of
 cytoplasmic components and as having a role in cancer pathogenesis.
 Aims: To investigate the anti-proliferative effects of atorvastatin
 on MCF-7 human breast adenocarcinoma cells with respect to both
 autophagy and apoptosis.
 Study Design: Cell culture study.
 Methods: Cell viability was analyzed using WST-1 cell proliferation
 assay. Apoptosis was determined by the TUNEL method, whereas
 autophagy was assessed by Beclin-1 and LC3B immunofluorescence
 staining. Ultrastructural analysis of cells was performed by electron
 microscopy.
 Results: Atorvastatin reduced MCF-7 cell proliferation in a dose- and
 time-dependent manner inducing TUNEL-, Beclin-1-, and LC3Bpositive
 cells. Moreover, ultrastructural analysis showed apoptotic,
 autophagic, and necrotic morphological changes in treatment groups.
 A statistically significant increase in the apoptotic index was detected
 with higher concentrations of atorvastatin at 24 h and 48 h (p<0.05).
 Conclusion: The anti-proliferative effects of atorvastatin on breast
 cancer cells is mediated by the induction of both apoptosis and
 autophagy which shows statins as a potential treatment option for
 breast cancer.

Makale detayları

Evaluation of the Cytotoxic and Autophagic Effects of Atorvastatin on MCF-7 Breast Cancer Cells

Yazarlar: Tuğba Alarcon MARTİNEZ | Naciye Dilara ZEYBEK | Sevda MÜFTÜOĞLU

Yayıncı: Trakya University

Dergi: Balkan Medical Journal 2018 Vol.35 Issue 3, pp.256-262

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000758790

Sıklık: İki Aylık

pISSN: 2146-3123

Sayı

Yazarlar:

Tuğba Alarcon MARTİNEZ | Naciye Dilara ZEYBEK | Sevda MÜFTÜOĞLU

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2018 Vol.35 Issue 3, pp.256-262

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000758790

Sıklık:

İki Aylık

pISSN:

2146-3123