Effects of Tarantula cubensis Extract on Experimental Acute Spinal Cord Injury in Rats

Serdar Aktaş; Zahir Kızılay; Nesibe Kahraman Çetin; Serap Ünübol Aypak; Murat Boyacıoğlu; Adem Yavaş

Makale detayları

Effects of Tarantula cubensis Extract on Experimental Acute Spinal Cord Injury in Rats

Yazarlar:

Serdar Aktaş | Zahir Kızılay | Nesibe Kahraman Çetin | Serap Ünübol Aypak | Murat Boyacıoğlu | Adem Yavaş

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.269-276

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293972

pISSN:

1308-1594

Anahtar kelimeler:

Özet:

 Spinal cord injury SCI is a critical health problem that occurs after spinal trauma. In this study, the possible effects of Tarantula cubensis extract in the acute period were evaluated. For the rat trauma model, 28 Sprague dawley rats were used. All rats were divided into 4 groups n7; Control, injury IG, prednisolone treatment PG, and Tarantula cubensis extract treatment TCG. Laminectomy was applied to all rats except the control group, and an aneurysm clamp was applied to the spinal cord for 60 seconds. BBB Basso, Beattie, Bresnahan Locomotor Rating Scale for clinical improvement assessment, histopathological inflammatory response, neuron viability, and the amount of biochemical parameters 8-hydroxydeoxyguanosine 8-OHdG, myeloperoxidase MPO, transforming growth factor beta 1 TGF β-1, tumor necrosis factor alpha TNF-α, interleukin 2 IL-2 were evaluated. According to histopathological evaluations; less damage and inflammation were observed in the TCG group compared to the IG group. There was a statistical similarity between TCG and PG p0.005 BBB score TCG values were higher than PG and IG rats. MPO level was found to be significantly lower than TCG group IG and PG in laminectomy rats p0.001. TGF β-1 ng-l, which is an anti-inflammatory cytokine, was measured at the highest level of TCG p0.001. TNF-α ng-l levels were higher in TCG, PG, and IG compared to the control group. IL-2 ng-l levels were decreased in TCG, PG, and control groups compared to the IG group p0.05. Tarantula cubensis extract has been determined neuroprotective effect by showing anti-inflammatory activity.

 Spinal cord injury SCI is a critical health problem that occurs after spinal trauma. In this study, the possible effects of Tarantula cubensis extract in the acute period were evaluated. For the rat trauma model, 28 Sprague dawley rats were used. All rats were divided into 4 groups n7; Control, injury IG, prednisolone treatment PG, and Tarantula cubensis extract treatment TCG. Laminectomy was applied to all rats except the control group, and an aneurysm clamp was applied to the spinal cord for 60 seconds. BBB Basso, Beattie, Bresnahan Locomotor Rating Scale for clinical improvement assessment, histopathological inflammatory response, neuron viability, and the amount of biochemical parameters 8-hydroxydeoxyguanosine 8-OHdG, myeloperoxidase MPO, transforming growth factor beta 1 TGF β-1, tumor necrosis factor alpha TNF-α, interleukin 2 IL-2 were evaluated. According to histopathological evaluations; less damage and inflammation were observed in the TCG group compared to the IG group. There was a statistical similarity between TCG and PG p0.005 BBB score TCG values were higher than PG and IG rats. MPO level was found to be significantly lower than TCG group IG and PG in laminectomy rats p0.001. TGF β-1 ng-l, which is an anti-inflammatory cytokine, was measured at the highest level of TCG p0.001. TNF-α ng-l levels were higher in TCG, PG, and IG compared to the control group. IL-2 ng-l levels were decreased in TCG, PG, and control groups compared to the IG group p0.05. Tarantula cubensis extract has been determined neuroprotective effect by showing anti-inflammatory activity.

 Spinal cord injury SCI is a critical health problem that occurs after spinal trauma. In this study, the possible effects of Tarantula cubensis extract in the acute period were evaluated. For the rat trauma model, 28 Sprague dawley rats were used. All rats were divided into 4 groups n7; Control, injury IG, prednisolone treatment PG, and Tarantula cubensis extract treatment TCG. Laminectomy was applied to all rats except the control group, and an aneurysm clamp was applied to the spinal cord for 60 seconds. BBB Basso, Beattie, Bresnahan Locomotor Rating Scale for clinical improvement assessment, histopathological inflammatory response, neuron viability, and the amount of biochemical parameters 8-hydroxydeoxyguanosine 8-OHdG, myeloperoxidase MPO, transforming growth factor beta 1 TGF β-1, tumor necrosis factor alpha TNF-α, interleukin 2 IL-2 were evaluated. According to histopathological evaluations; less damage and inflammation were observed in the TCG group compared to the IG group. There was a statistical similarity between TCG and PG p0.005 BBB score TCG values were higher than PG and IG rats. MPO level was found to be significantly lower than TCG group IG and PG in laminectomy rats p0.001. TGF β-1 ng-l, which is an anti-inflammatory cytokine, was measured at the highest level of TCG p0.001. TNF-α ng-l levels were higher in TCG, PG, and IG compared to the control group. IL-2 ng-l levels were decreased in TCG, PG, and control groups compared to the IG group p0.05. Tarantula cubensis extract has been determined neuroprotective effect by showing anti-inflammatory activity.

 Omurilik yaralanması SCI, omurganın ciddi yaralanmalarından sonra ortaya çıkan kritik bir sağlık sorunudur. Bu çalışmada Tarantula cubensis ekstraktının akut dönemdeki olası etkileri değerlendirilmiştir. Sıçan travma modeli için 28 Sprague dawley kullanıldı. Tüm ratlar 4 gruba ayrıldı n7: Kontrol, hasar grubu IG, prednizolon tedavisi PG ve Tarantula cubensis ekstraktı tedavisi TCG. Kontrol grubu dışındaki tüm ratlara laminektomi uygulandı ve spinal korda 60 saniye anevrizma klempi uygulandı. Klinik iyileştirme değerlendirmesi, BBB Basso, Beattie, Bresnahan Locomotor Rating Scale, histopatolojik inflamatuar yanıt, nöron canlılığı ve biyokimyasal değerlendirmede için 8- hydroxydeoxyguanosine 8-OHdG, myeloperoxidase MPO, transforming growth faktör beta 1 TGF β-1, tümor Nekroz Faktör Alfa TNF-α, interleukin-2 IL-2 değerlendirildi. Histopatolojik değerlendirmelere göre; TCG grubunda IG grubuna oranla daha az hasar ve inflamasyon gözlendi. TCG ve PG arasında istatistiksel olarak benzerlik vardı p0.005. BBB skoru TCG değerleri PG ve IGye göre yüksek bulundu. Laminektomi yapılan sıçanlarda MPO düzeyi TCGde IG ve PGden anlamlı düzeyde düşük bulundu p0.001. Antiinflamatuvar bir sitokin olan TGF β-1 ng-l TCGde en yüksek seviyesinde ölçüldü p0.001. TNF-α ng-l seviyeleri TCG, PG ve IGde kontrol grubuna göre daha yüksekti. Ancak kendi aralarında anlamlı bulunmamışlardır. IL-2 ng-l düzeyleri TCG, PGde IG grubuna göre azalmıştı p0.05. Sonuç olarak; Tarantula cubensis ekstraktının antiinflamatuar aktivite göstererek omurilik dokusunda nöroprotektif etkisi olduğu belirlenmiştir.

Makale detayları

Effects of Tarantula cubensis Extract on Experimental Acute Spinal Cord Injury in Rats

Yazarlar:

Serdar Aktaş | Zahir Kızılay | Nesibe Kahraman Çetin | Serap Ünübol Aypak | Murat Boyacıoğlu | Adem Yavaş

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.269-276

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293972

pISSN:

1308-1594

Anti-inflammatory effect

Neuroprotection

Spinal cord injury

Tarantula cubensis extractAntiinflamatuar etki

Nöroproteksiyon

Omurilik yaralanması

Tarantula cubensis ekstraktı

Sayı

Makale detayları

Yazarlar: Serdar Aktaş | Zahir Kızılay | Nesibe Kahraman Çetin | Serap Ünübol Aypak | Murat Boyacıoğlu | Adem Yavaş

Yayıncı: Afyon Kocatepe University

Ana Başlıklar: Veteriner Cerrahi

Dergi: Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.269-276

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293972

pISSN: 1308-1594

Sayı

Yazarlar:

Serdar Aktaş | Zahir Kızılay | Nesibe Kahraman Çetin | Serap Ünübol Aypak | Murat Boyacıoğlu | Adem Yavaş

Yayıncı:

Afyon Kocatepe University

Ana Başlıklar:

Veteriner Cerrahi

Dergi:

Kocatepe Veteriner Dergisi Vol.16 Issue 3, pp.269-276

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293972

pISSN:

1308-1594