The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

Onur Akı; Ebubekir Yüksel; Mustafa İmren; Refik Bozbuğa; Ramazan Canhilal

Makale detayları

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

Yazarlar:

Onur Akı | Ebubekir Yüksel | Mustafa İmren | Refik Bozbuğa | Ramazan Canhilal

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Entomoloji

Dergi:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.176-185

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293834

pISSN:

2149-8245

Anahtar kelimeler:

Özet:

 Entomopathogenic nematodes EPNs Rhabditida: Heterorhabditidae and Steinernematidae are effective biocontrol agents for many insect pests and are generally stored for a period of time prior to their use in the laboratory or field. However, unfavorable storage conditions have a great impact on the survival and infectivity of EPNs. This study was conducted to determine the optimum storage conditions of infective juveniles IJs of four native EPN species Heterorhabditis bacteriophora FLH-4H, H. indica 216-H, Steinernema feltiae KCS-S, and S. bicornotum MGZ-4S under laboratory conditions. The survival capability of the IJs was tested at different concentrations 500, 1000, 1500, and 2000 IJs, temperatures 9 and 25 C and storage media double-distilled water ddH2O, tap water, and sterile Ringer solution. In general, the survival of IJs of tested EPN species was the highest at the 1st month after treatment MAT at the concentrations of 1000 and 1500 IJs and gradually decreased with the increasing storage periods. The survival rates of the IJs of Steirnematids were generally higher than Heterorhabditid species. The highest survival of IJs was generally obtained after 1-month storage in Ringer solution at 9C while tap water led to poor survival for the IJs at both temperatures tested. The IJs that were stored at 9C induced higher mortalities on the larvae of Galleria mellonella L. Lepidoptera: Pyralidae. The results showed that the IJs of tested EPN species can remain viable for a longer period of time in Ringer solution at 1000 and 1500 IJs concentrations at 9C without losing much of their infectivity.

 Entomopathogenic nematodes EPNs Rhabditida: Heterorhabditidae and Steinernematidae are effective biocontrol agents for many insect pests and are generally stored for a period of time prior to their use in the laboratory or field. However, unfavorable storage conditions have a great impact on the survival and infectivity of EPNs. This study was conducted to determine the optimum storage conditions of infective juveniles IJs of four native EPN species Heterorhabditis bacteriophora FLH-4H, H. indica 216-H, Steinernema feltiae KCS-S, and S. bicornotum MGZ-4S under laboratory conditions. The survival capability of the IJs was tested at different concentrations 500, 1000, 1500, and 2000 IJs, temperatures 9 and 25 C and storage media double-distilled water ddH2O, tap water, and sterile Ringer solution. In general, the survival of IJs of tested EPN species was the highest at the 1st month after treatment MAT at the concentrations of 1000 and 1500 IJs and gradually decreased with the increasing storage periods. The survival rates of the IJs of Steirnematids were generally higher than Heterorhabditid species. The highest survival of IJs was generally obtained after 1-month storage in Ringer solution at 9C while tap water led to poor survival for the IJs at both temperatures tested. The IJs that were stored at 9C induced higher mortalities on the larvae of Galleria mellonella L. Lepidoptera: Pyralidae. The results showed that the IJs of tested EPN species can remain viable for a longer period of time in Ringer solution at 1000 and 1500 IJs concentrations at 9C without losing much of their infectivity.

 Entomopathogenic nematodes EPNs Rhabditida: Heterorhabditidae and Steinernematidae are effective biocontrol agents for many insect pests and are generally stored for a period of time prior to their use in the laboratory or field. However, unfavorable storage conditions have a great impact on the survival and infectivity of EPNs. This study was conducted to determine the optimum storage conditions of infective juveniles IJs of four native EPN species Heterorhabditis bacteriophora FLH-4H, H. indica 216-H, Steinernema feltiae KCS-S, and S. bicornotum MGZ-4S under laboratory conditions. The survival capability of the IJs was tested at different concentrations 500, 1000, 1500, and 2000 IJs, temperatures 9 and 25 C and storage media double-distilled water ddH2O, tap water, and sterile Ringer solution. In general, the survival of IJs of tested EPN species was the highest at the 1st month after treatment MAT at the concentrations of 1000 and 1500 IJs and gradually decreased with the increasing storage periods. The survival rates of the IJs of Steirnematids were generally higher than Heterorhabditid species. The highest survival of IJs was generally obtained after 1-month storage in Ringer solution at 9C while tap water led to poor survival for the IJs at both temperatures tested. The IJs that were stored at 9C induced higher mortalities on the larvae of Galleria mellonella L. Lepidoptera: Pyralidae. The results showed that the IJs of tested EPN species can remain viable for a longer period of time in Ringer solution at 1000 and 1500 IJs concentrations at 9C without losing much of their infectivity.

 Entomopatojen nematodlar EPN Rhabditida: Heterorhabditidae ve Steinernematidae birçok zararlı böceklerin etkili biyokontrol ajanlarıdır ve genellikle laboratuvarda veya arazide kullanılmadan önce belirli bir süre depolanırlar. Bununla birlikte, elverişsiz saklama koşullarının EPNlerin hayatta kalması ve infektivitesi üzerinde büyük bir etkisi vardır. Bu çalışma, dört yerel EPN türünün Heterorhabditis bacteriophora FLH-4H, H. indica 216-H, Steinernema feltiae KCS-4S ve S. bicornotum MGZ-4S infektif juvenillerinin IJ laboratuvar koşullarında optimum saklama koşullarını belirlemek için gerçekleştirilmiştir. IJlerin canlılıkları, farklı konsantrasyonlarda 500, 1000, 1500 ve 2000 IJ, sıcaklıklarda 9 ve 25 C ve depolama ortamında ddH2O, musluk suyu ve steril Ringer solüsyonu test edilmiştir. Genel olarak, test edilen EPN türlere ait IJlerin canlılıkları, 1000 ve 1500 IJ konsantrasyonlarında depolamadan sonraki 1. ayda en yüksek iken artan depolama süreleri ile kademeli olarak azalmıştır. Genel olarak Steirnematidlerin IJlerinin hayatta kalma oranları Heterorhabditid türlerinden daha yüksek olduğu belirlenmiştir. IJlerin en yüksek hayatta kalma oranı, genellikle Ringer solüsyonunda 9Cde 1 aylık depolamadan sonra elde edilirken, musluk suyu, test edilen her iki sıcaklıkta da IJler canlılıklarında önemli bir düşüşe neden olmuştur. Patojenite testlerinde, 9Cde depolanan IJler, Galleria mellonella L. Lepidoptera: Pyralidae larvalarında daha yüksek ölüm oranlarına neden olmuştur. Sonuçlar, test edilen EPN türlerine ait IJlerin, infektivitelerinde ciddi bir düşüş olmadan 9Cde, 1000 ve 1500 IJs konsantrasyonlarında, Ringer solüsyonunda daha uzun süre canlı kalabildiğini göstermiştir.

Makale detayları

The Role of Storage Duration and Conditions on the Survival and Pathogenicity of Entomopathogenic Nematodes

Yazarlar:

Onur Akı | Ebubekir Yüksel | Mustafa İmren | Refik Bozbuğa | Ramazan Canhilal

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Entomoloji

Dergi:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.176-185

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293834

pISSN:

2149-8245

Storage

Beneficial Nematodes

Biological Control

Sustainable AgricultureDepolama

Faydalı Nematodlar

Biyolojik Mücadele

Sürdürülebilir Tarım

Sayı

Makale detayları

Yazarlar: Onur Akı | Ebubekir Yüksel | Mustafa İmren | Refik Bozbuğa | Ramazan Canhilal

Yayıncı: Bolu Abant Izzet Baysal University

Ana Başlıklar: Entomoloji

Dergi: Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.176-185

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293834

pISSN: 2149-8245

Sayı

Yazarlar:

Onur Akı | Ebubekir Yüksel | Mustafa İmren | Refik Bozbuğa | Ramazan Canhilal

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Entomoloji

Dergi:

Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Vol.9 Issue 2, pp.176-185

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293834

pISSN:

2149-8245