Molecular Analysis of Capsicum Lines for Resistance to Tomato Spotted Wilt Virus (TSWV)

Hüseyin Basım; Osman Kandil; Cansu Ökem

Makale detayları

Molecular Analysis of Capsicum Lines for Resistance to Tomato Spotted Wilt Virus (TSWV)

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359194

pISSN:

2791-8807

Anahtar kelimeler:

Özet:

 This study was conducted to determine the molecular resistance status of 35 different Capsicum pepper lines against Tomato Spotted Wilt Virus TSWV. PCR amplification was performed using the SCAC₅₆₈ CAPS marker targeting the tsw resistance gene. The PCR products were digested with the XbaI restriction enzyme and subsequently analyzed by agarose gel electrophoresis. Based on the resulting band patterns, the genotypes were classified, revealing that 22 lines were heterozygous resistant Rr, while 13 lines were susceptible rr. The identified resistant individuals will be subjected to further selection and pathogenicity testing and integrated into hybrid breeding programs. This study contributes to local seed improvement efforts and demonstrates that marker-assisted selection is an effective and reliable strategy for developing TSWV-resistant Capsicum cultivars.

 This study was conducted to determine the molecular resistance status of 35 different Capsicum pepper lines against Tomato Spotted Wilt Virus TSWV. PCR amplification was performed using the SCAC₅₆₈ CAPS marker targeting the tsw resistance gene. The PCR products were digested with the XbaI restriction enzyme and subsequently analyzed by agarose gel electrophoresis. Based on the resulting band patterns, the genotypes were classified, revealing that 22 lines were heterozygous resistant Rr, while 13 lines were susceptible rr. The identified resistant individuals will be subjected to further selection and pathogenicity testing and integrated into hybrid breeding programs. This study contributes to local seed improvement efforts and demonstrates that marker-assisted selection is an effective and reliable strategy for developing TSWV-resistant Capsicum cultivars.

 This study was conducted to determine the molecular resistance status of 35 different Capsicum pepper lines against Tomato Spotted Wilt Virus TSWV. PCR amplification was performed using the SCAC₅₆₈ CAPS marker targeting the tsw resistance gene. The PCR products were digested with the XbaI restriction enzyme and subsequently analyzed by agarose gel electrophoresis. Based on the resulting band patterns, the genotypes were classified, revealing that 22 lines were heterozygous resistant Rr, while 13 lines were susceptible rr. The identified resistant individuals will be subjected to further selection and pathogenicity testing and integrated into hybrid breeding programs. This study contributes to local seed improvement efforts and demonstrates that marker-assisted selection is an effective and reliable strategy for developing TSWV-resistant Capsicum cultivars.

 Bu çalışma, 35 farklı Capsicum biber hattının Domates Benekli Solgunluk Virüsüne TSWV karşı moleküler dayanıklılık durumunu belirlemek amacıyla yürütülmüştür. PCR amplifikasyonu, tsw dayanıklılık genini hedef alan SCAC₅₆₈ CAPS markörü kullanılarak gerçekleştirilmiştir. PCR ürünleri XbaI restriksiyon enzimi ile kesilmiş ve daha sonra agaroz jel elektroforezi ile analiz edilmiştir. Elde edilen DNA bant örüntülerine dayanarak, genotipler sınıflandırılmış ve 22 biber hattının heterozigot dayanıklı Rr, 13 hattın ise duyarlı rr olduğu ortaya çıkmıştır. Belirlenen dayanıklı bireyler daha ileri seleksiyon ve patojenite testlerine tabi tutulacak ve melez ıslah programlarına entegre edilecektir. Bu çalışma, yerel tohum ıslah çalışmalarına katkıda bulunmakta ve markör destekli seleksiyonun TSWVye dayanıklı Capsicum çeşitlerinin geliştirilmesi için etkili ve güvenilir bir strateji olduğunu göstermektedir.

Makale detayları

Molecular Analysis of Capsicum Lines for Resistance to Tomato Spotted Wilt Virus (TSWV)

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359194

pISSN:

2791-8807

Capsicum

MAS

SCAC568

tsw

TSWVMAS

SCAC568

tsw

Biber

TSWV

Sayı

Makale detayları

Yazarlar: Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı: Bingol University

Ana Başlıklar: Fitopatoloji

Dergi: Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.1-10

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359194

pISSN: 2791-8807

Sayı

Yazarlar:

Hüseyin Basım | Osman Kandil | Cansu Ökem

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359194

pISSN:

2791-8807