Induction of variability through mutagenesis in opium poppy (Papaver somniferum L.)

Avijeet CHATTERJEE; Sudhir SHUKLA; Brij KISHORE MISHRA; Anu RASTOGI; Sant PRASAD SINGH

Makale detayları

Induction of variability through mutagenesis in opium poppy (Papaver somniferum L.)

Yazarlar:

Avijeet CHATTERJEE | Sudhir SHUKLA | Brij KISHORE MISHRA | Anu RASTOGI | Sant PRASAD SINGH

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2012 Vol.36 Issue 1, pp.1-11

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000807979

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Opium poppy (Papaver somniferum L.), an important plant of the family Papaveraceae, is valued for its extensive medicinal properties due to the presence of more than 80 different alkaloids, which are products of the polygenic interaction of different genes involved in plant metabolism. Broadening the genetic base through induced mutation is a supplementary tool that can lead to the development of genetic variability. The present study was undertaken to generate a broad genetic variability through mutation breeding using physical doses (gamma radiation of kR10 to kR50 at an interval of kR10), chemical doses (EMS of 0.2, 0.4, 0.6, and 0.8% (w/v)), and combined doses (gamma and EMS) of mutagen, and to evaluate the plants’ advance generations for different traits as well as for specific alkaloids, especially thebaine and codeine. The kR30 dose, which caused the highest results for all 3 genetic parameters (GCV, h2, and GA%) for 7 traits, was the most effective in NBRI-1. Similarly, the kR10 + 0.4% EMS dose proved to be the best for NBRI-5, affecting 10 different characters. The kR10 + 0.4% EMS dose created positive mutations for high thebaine and codeine content and low morphine content, while the kR40 + 0.6% EMS dose did the same for narcotine. The study also confirmed that the pathway of morphinan alkaloids and narcotine formation was bifurcated at the lower combined dose (kR30 and kR10 + 0.4% EMS), which was effective in causing micromutation in morphinan alkaloid pathways. The higher combined dose (kR40 + 0.4% EMS) affected narcotine production.

 

Opium poppy (Papaver somniferum L.), an important plant of the family Papaveraceae, is valued for its extensive medicinal properties due to the presence of more than 80 different alkaloids, which are products of the polygenic interaction of different genes involved in plant metabolism. Broadening the genetic base through induced mutation is a supplementary tool that can lead to the development of genetic variability. The present study was undertaken to generate a broad genetic variability through mutation breeding using physical doses (gamma radiation of kR10 to kR50 at an interval of kR10), chemical doses (EMS of 0.2, 0.4, 0.6, and 0.8% (w/v)), and combined doses (gamma and EMS) of mutagen, and to evaluate the plants’ advance generations for different traits as well as for specific alkaloids, especially thebaine and codeine. The kR30 dose, which caused the highest results for all 3 genetic parameters (GCV, h2, and GA%) for 7 traits, was the most effective in NBRI-1. Similarly, the kR10 + 0.4% EMS dose proved to be the best for NBRI-5, affecting 10 different characters. The kR10 + 0.4% EMS dose created positive mutations for high thebaine and codeine content and low morphine content, while the kR40 + 0.6% EMS dose did the same for narcotine. The study also confirmed that the pathway of morphinan alkaloids and narcotine formation was bifurcated at the lower combined dose (kR30 and kR10 + 0.4% EMS), which was effective in causing micromutation in morphinan alkaloid pathways. The higher combined dose (kR40 + 0.4% EMS) affected narcotine production.

Makale detayları

Induction of variability through mutagenesis in opium poppy (Papaver somniferum L.)

Yazarlar:

Avijeet CHATTERJEE | Sudhir SHUKLA | Brij KISHORE MISHRA | Anu RASTOGI | Sant PRASAD SINGH

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2012 Vol.36 Issue 1, pp.1-11

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000807979

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Alkaloids

genetic variability

induced mutation

Papaver somniferum

thebaine

Sayı

Makale detayları

Induction of variability through mutagenesis in opium poppy (Papaver somniferum L.)

Yazarlar: Avijeet CHATTERJEE | Sudhir SHUKLA | Brij KISHORE MISHRA | Anu RASTOGI | Sant PRASAD SINGH

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2012 Vol.36 Issue 1, pp.1-11

Belge Tipi: Makale

Yayın Tarihi: 12/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000807979

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Alkaloids

genetic variability

induced mutation

Papaver somniferum

thebaine

Sayı

Yazarlar:

Avijeet CHATTERJEE | Sudhir SHUKLA | Brij KISHORE MISHRA | Anu RASTOGI | Sant PRASAD SINGH

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2012 Vol.36 Issue 1, pp.1-11

Belge Tipi:

Makale

Yayın Tarihi:

12/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000807979

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X