Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock

Hasan ÖNGÖR; Necati TİMURKAAN; Fethiye ÇÖVEN; Burak KARABULUT; Hatice ERÖKSÜZ; Burhan ÇETİNKAYA; Tayfun CARLI

doi:https://doi.org/10.33988/auvfd.756970

Makale detayları

Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock

Yazarlar:

Hasan ÖNGÖR | Necati TİMURKAAN | Fethiye ÇÖVEN | Burak KARABULUT | Hatice ERÖKSÜZ | Burhan ÇETİNKAYA | Tayfun CARLI

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2021 Vol.68 Issue 2, pp.167-172

Belge Tipi:

Makale

Yayın Tarihi:

3/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000849369

DOI:

https://doi.org/10.33988/auvfd.756970

Özet:

 

The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.

Makale detayları

Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock

Yazarlar: Hasan ÖNGÖR | Necati TİMURKAAN | Fethiye ÇÖVEN | Burak KARABULUT | Hatice ERÖKSÜZ | Burhan ÇETİNKAYA | Tayfun CARLI

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2021 Vol.68 Issue 2, pp.167-172

Belge Tipi: Makale

Yayın Tarihi: 3/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000849369

DOI: https://doi.org/10.33988/auvfd.756970

Sayı

Yazarlar:

Hasan ÖNGÖR | Necati TİMURKAAN | Fethiye ÇÖVEN | Burak KARABULUT | Hatice ERÖKSÜZ | Burhan ÇETİNKAYA | Tayfun CARLI

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2021 Vol.68 Issue 2, pp.167-172

Belge Tipi:

Makale

Yayın Tarihi:

3/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000849369

DOI:

https://doi.org/10.33988/auvfd.756970