Effects of Curcumin Treatment on Cell Energy Status, Levels of Mitochondrial Enzymes, and Gene Expression of Glucose-related Mechanism in Pancreatic Cancer Cell Lines

Emine Nedime Korucu; Esma Menevşe; Dudu Erkoç Kaya; Fatma Gokturk; Hilal Arikoglu

Makale detayları

Effects of Curcumin Treatment on Cell Energy Status, Levels of Mitochondrial Enzymes, and Gene Expression of Glucose-related Mechanism in Pancreatic Cancer Cell Lines

Yazarlar:

Emine Nedime Korucu | Esma Menevşe | Dudu Erkoç Kaya | Fatma Gokturk | Hilal Arikoglu

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.70-84

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299971

pISSN:

2564-7288

Anahtar kelimeler:

Özet:

 Background and Purpose:. Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. It was aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells. Methods: BxPC-3 and Panc-1 cells were incubated, were replaced with containing various concentrations of curcumin 10-125 μM for 24 h. Cell lysate Adenosine triphosphate ATP, Adenosine diphosphate ADP, Adenosine monophosphate AMP, Manganese superoxidase MnSOD, and cytochrome p450 reductase CPR concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase LDH, mitochondrially encoded ATP synthase membrane subunit 6 MTATP6, Glucose transporter 1 GLUT1, and cytochrome p450 were analyzed. Results and Conclusion: IC50 values for 24 hours were found as 47,26 μM in BxPC-3 and 45,84 μM in Panc-1 cells. Treatment with curcumin inhibits oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but it showed an increase in Panc-1 supplemented with curcumin. The effects of curcumin on GLUT-1 are significantly important at a dose of curcumin of 45 μM concentration and affect glucose consumption in both cells. Curcumin showed anti-proliferative, and antioxidant effects.

 Background and Purpose:. Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. It was aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells. Methods: BxPC-3 and Panc-1 cells were incubated, were replaced with containing various concentrations of curcumin 10-125 μM for 24 h. Cell lysate Adenosine triphosphate ATP, Adenosine diphosphate ADP, Adenosine monophosphate AMP, Manganese superoxidase MnSOD, and cytochrome p450 reductase CPR concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase LDH, mitochondrially encoded ATP synthase membrane subunit 6 MTATP6, Glucose transporter 1 GLUT1, and cytochrome p450 were analyzed. Results and Conclusion: IC50 values for 24 hours were found as 47,26 μM in BxPC-3 and 45,84 μM in Panc-1 cells. Treatment with curcumin inhibits oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but it showed an increase in Panc-1 supplemented with curcumin. The effects of curcumin on GLUT-1 are significantly important at a dose of curcumin of 45 μM concentration and affect glucose consumption in both cells. Curcumin showed anti-proliferative, and antioxidant effects.

 Background and Purpose:. Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. It was aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells. Methods: BxPC-3 and Panc-1 cells were incubated, were replaced with containing various concentrations of curcumin 10-125 μM for 24 h. Cell lysate Adenosine triphosphate ATP, Adenosine diphosphate ADP, Adenosine monophosphate AMP, Manganese superoxidase MnSOD, and cytochrome p450 reductase CPR concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase LDH, mitochondrially encoded ATP synthase membrane subunit 6 MTATP6, Glucose transporter 1 GLUT1, and cytochrome p450 were analyzed. Results and Conclusion: IC50 values for 24 hours were found as 47,26 μM in BxPC-3 and 45,84 μM in Panc-1 cells. Treatment with curcumin inhibits oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but it showed an increase in Panc-1 supplemented with curcumin. The effects of curcumin on GLUT-1 are significantly important at a dose of curcumin of 45 μM concentration and affect glucose consumption in both cells. Curcumin showed anti-proliferative, and antioxidant effects.

 Background and Purpose:. Curcumin is an active component of turmeric, has antitumor, immunomodulatory, anti-inflammatory effects. It was aimed to investigate the effects of the administration of curcumin on the energy metabolism, the abnormal redox defense mechanism profile, the malignant transformation indicator of Panc-1 and BxPC-3 pancreatic cancer cells. Methods: BxPC-3 and Panc-1 cells were incubated, were replaced with containing various concentrations of curcumin 10-125 μM for 24 h. Cell lysate Adenosine triphosphate ATP, Adenosine diphosphate ADP, Adenosine monophosphate AMP, Manganese superoxidase MnSOD, and cytochrome p450 reductase CPR concentrations were analyzed with HPLC and ELISA methods. Genes expression of Lactate dehydrogenase LDH, mitochondrially encoded ATP synthase membrane subunit 6 MTATP6, Glucose transporter 1 GLUT1, and cytochrome p450 were analyzed. Results and Conclusion: IC50 values for 24 hours were found as 47,26 μM in BxPC-3 and 45,84 μM in Panc-1 cells. Treatment with curcumin inhibits oxidative stress by increasing MnSOD enzyme levels. ATP levels did not change in BxPC-3 cells, but it showed an increase in Panc-1 supplemented with curcumin. The effects of curcumin on GLUT-1 are significantly important at a dose of curcumin of 45 μM concentration and affect glucose consumption in both cells. Curcumin showed anti-proliferative, and antioxidant effects.

Makale detayları

Effects of Curcumin Treatment on Cell Energy Status, Levels of Mitochondrial Enzymes, and Gene Expression of Glucose-related Mechanism in Pancreatic Cancer Cell Lines

Yazarlar: Emine Nedime Korucu | Esma Menevşe | Dudu Erkoç Kaya | Fatma Gokturk | Hilal Arikoglu

Yayıncı: Dokuz Eylul University

Ana Başlıklar: Health Care Administration

Dergi: Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.70-84

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001299971

pISSN: 2564-7288

Panc-1

BxPC-3

curcumin

MnSOD

ATP

CPR

Sayı

Yazarlar:

Emine Nedime Korucu | Esma Menevşe | Dudu Erkoç Kaya | Fatma Gokturk | Hilal Arikoglu

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.70-84

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299971

pISSN:

2564-7288