VARIATIONS IN HEAT SHOCK PROTEINS BETWEEN DIFFERENT HONEY BEES AND BEE TAXA UTILIZING BIOINFORMATICS

Hossam Abou-shaara

Makale detayları

VARIATIONS IN HEAT SHOCK PROTEINS BETWEEN DIFFERENT HONEY BEES AND BEE TAXA UTILIZING BIOINFORMATICS

Yazarlar:

Hossam Abou-shaara

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Entomoloji

Dergi:

Uludağ Arıcılık Dergisi Vol.24 Issue 1, pp.38-52

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318058

pISSN:

2687-5594

Anahtar kelimeler:

Özet:

 The changes in climate and exposure to heat stress are major concerns for agricultural communities as it affects pollinators like bees. Bees from different taxa play a crucial role in plant pollination, and their exposure to heat stress induces the expression of heat shock proteins HSPs to protect their cells. Several studies have analyzed the variations in HSPs expression levels and amino acid sequences. Databases for sequences of HSPs with different molecular weights are currently available. Variations in HSPs expression levels have been noted among individuals belonging to the same or different bee taxa exposed to heat stress. The properties of HSPs could help in understanding these variations. This study utilized bioinformatics and protein analysis tools to investigate the variations in sequences of heat shock proteins 60 HSP60 and 83 HSP83 in 18 bee taxa 15 from Family Apidae, 2 from Family Halictidae, and one from Megachilidae. The analysis showed some identical values to bees from genus Apis and Bombus. For HSP60, all bee taxa had high G content 587-602, followed by A 438-444, then C 389-404, and finally T 282-291. For HSP83, all bee taxa had high A content 730-759, followed by G 572-592, then C 406-419, and finally T 415-429. The conserved domains were highly identical in case of HSP60 versus HSP83. The motifs were from one or more protein families with variation among taxa. All proteins showed hydrophilic properties with variable isoelectric points. The study suggested an identical 3-D structure for proteins in all bee taxa. The role of the detected variations in affecting the response of HSPs to stress was discussed. This study paves the way for more investigations on HSPs and encourages the use of bioinformatics and protein analysis tools to explain any observable variations.

 The changes in climate and exposure to heat stress are major concerns for agricultural communities as it affects pollinators like bees. Bees from different taxa play a crucial role in plant pollination, and their exposure to heat stress induces the expression of heat shock proteins HSPs to protect their cells. Several studies have analyzed the variations in HSPs expression levels and amino acid sequences. Databases for sequences of HSPs with different molecular weights are currently available. Variations in HSPs expression levels have been noted among individuals belonging to the same or different bee taxa exposed to heat stress. The properties of HSPs could help in understanding these variations. This study utilized bioinformatics and protein analysis tools to investigate the variations in sequences of heat shock proteins 60 HSP60 and 83 HSP83 in 18 bee taxa 15 from Family Apidae, 2 from Family Halictidae, and one from Megachilidae. The analysis showed some identical values to bees from genus Apis and Bombus. For HSP60, all bee taxa had high G content 587-602, followed by A 438-444, then C 389-404, and finally T 282-291. For HSP83, all bee taxa had high A content 730-759, followed by G 572-592, then C 406-419, and finally T 415-429. The conserved domains were highly identical in case of HSP60 versus HSP83. The motifs were from one or more protein families with variation among taxa. All proteins showed hydrophilic properties with variable isoelectric points. The study suggested an identical 3-D structure for proteins in all bee taxa. The role of the detected variations in affecting the response of HSPs to stress was discussed. This study paves the way for more investigations on HSPs and encourages the use of bioinformatics and protein analysis tools to explain any observable variations.

 The changes in climate and exposure to heat stress are major concerns for agricultural communities as it affects pollinators like bees. Bees from different taxa play a crucial role in plant pollination, and their exposure to heat stress induces the expression of heat shock proteins HSPs to protect their cells. Several studies have analyzed the variations in HSPs expression levels and amino acid sequences. Databases for sequences of HSPs with different molecular weights are currently available. Variations in HSPs expression levels have been noted among individuals belonging to the same or different bee taxa exposed to heat stress. The properties of HSPs could help in understanding these variations. This study utilized bioinformatics and protein analysis tools to investigate the variations in sequences of heat shock proteins 60 HSP60 and 83 HSP83 in 18 bee taxa 15 from Family Apidae, 2 from Family Halictidae, and one from Megachilidae. The analysis showed some identical values to bees from genus Apis and Bombus. For HSP60, all bee taxa had high G content 587-602, followed by A 438-444, then C 389-404, and finally T 282-291. For HSP83, all bee taxa had high A content 730-759, followed by G 572-592, then C 406-419, and finally T 415-429. The conserved domains were highly identical in case of HSP60 versus HSP83. The motifs were from one or more protein families with variation among taxa. All proteins showed hydrophilic properties with variable isoelectric points. The study suggested an identical 3-D structure for proteins in all bee taxa. The role of the detected variations in affecting the response of HSPs to stress was discussed. This study paves the way for more investigations on HSPs and encourages the use of bioinformatics and protein analysis tools to explain any observable variations.

 İklimdeki değişiklikler ve tozlaştırıcıların ısı stresine maruz kalması, tarım topluluklarının en büyük endişeleri arasında yer alıyor. Farklı taksonlara ait arıların bitkilerin tozlaşmasında büyük rolü vardır. Arıların ısı stresine maruz kalması, vücut hücrelerini korumak için ısı şoku proteinlerinin HSPler ekspresyonuna neden olur. Birçok çalışma HSPlerin ekspresyon seviyelerindeki farklılıkları araştırmış ve amino asit dizilerini analiz etmiştir. Şu anda, farklı molekül ağırlıklarına sahip HSP dizileri için veritabanları mevcuttur. Aynı arı taksonuna ait veya ısı stresine maruz kalan farklı taksonlara ait bireyler arasında HSPlerin ekspresyon seviyelerindeki farklılıklar kaydedilmiştir. HSPlerin özelliklerinin bu tür farklılıkların anlaşılmasına yardımcı olabileceği varsayılmaktadır. Bu çalışmada, 18 arı taksonunda 15i Apidae familyasından, 2si Halictidae familyasından ve bir Megachilidaeden. Amino asitlerin ve nükleotidlerin analizi, Apis ve Bombus cinsinden arılarla aynı değerleri gösterdi. Korunan alanlar, HSP60 durumunda HSP83e göre oldukça özdeşti. Motifler, taksonlar arasında çeşitlilik gösteren bir veya daha fazla protein ailesindendi. Tüm proteinler değişken izoelektrik noktalarla hidrofilik özellikler gösterdi. Çalışma, tüm arı taksonlarındaki proteinler için aynı 3 boyutlu yapıyı öne sürdü. Tespit edilen varyasyonların HSPlerin strese tepkisini etkilemedeki rolü tartışıldı. Bu çalışma HSPler hakkında daha fazla araştırmanın önünü açıyor ve gözlemlenebilir varyasyonları açıklamak için biyoinformatik ve protein analiz araçlarının kullanımını teşvik ediyor.

Makale detayları

VARIATIONS IN HEAT SHOCK PROTEINS BETWEEN DIFFERENT HONEY BEES AND BEE TAXA UTILIZING BIOINFORMATICS

Yazarlar:

Hossam Abou-shaara

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Entomoloji

Dergi:

Uludağ Arıcılık Dergisi Vol.24 Issue 1, pp.38-52

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318058

pISSN:

2687-5594

Honey bees

Bioinformatics

Stress

Climate

HSPBal arıları

Biyoinformatik

Stres

İklim

HSP

Sayı

Makale detayları

Yazarlar: Hossam Abou-shaara

Yayıncı: Bursa Uludağ University

Ana Başlıklar: Entomoloji

Dergi: Uludağ Arıcılık Dergisi Vol.24 Issue 1, pp.38-52

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001318058

pISSN: 2687-5594

Sayı

Yazarlar:

Hossam Abou-shaara

Yayıncı:

Bursa Uludağ University

Ana Başlıklar:

Entomoloji

Dergi:

Uludağ Arıcılık Dergisi Vol.24 Issue 1, pp.38-52

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318058

pISSN:

2687-5594