Phylogenetic Analysis and Genetic Characterization of Bovine Respiratory Syncytial Virus G Glycoprotein / Bovine Respiratory Syncytial Virus G Glycoproteininin Filogenetik ve Analizi ve Genetik Karakterizasyonu

Ali Küçük; Özge Sevinç Korkmaz Akar; Yakup Yıldırım; Kamil Atlı

Makale detayları

Phylogenetic Analysis and Genetic Characterization of Bovine Respiratory Syncytial Virus G Glycoprotein / Bovine Respiratory Syncytial Virus G Glycoproteininin Filogenetik ve Analizi ve Genetik Karakterizasyonu

Yazarlar:

Ali Küçük | Özge Sevinç Korkmaz Akar | Yakup Yıldırım | Kamil Atlı

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Veteriner Viroloji

Dergi:

Antakya Veteriner Bilimleri Dergisi Vol.3 Issue 2, pp.32-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334275

pISSN:

2979-9805

Anahtar kelimeler:

Özet:

 This study aimed to molecularly detect Bovine Respiratory Syncytial Virus BRSV in cattle with clinical signs of respiratory system infection and to conduct a phylogenetic analysis of the detected strains. To detect the pathogen, nasal swab samples collected from 96 cattle were subjected to Reverse Transcriptase  nested PCR using specific primer sets. As a result of the molecular techniques, viral nucleic acid was detected in 2.08 296 of the samples. Additionally, sequence and phylogenetic analyses of the detected local variant were performed and compared with various BRSV strains identified in different regions of Turkey and around the world. The genetic data obtained revealed that the isolates identified in this study belong to Subgroup III, alongside BRSV strains reported from various regions of Turkey, France, and the USA. Immunologically significant point mutations were detected in the immunodominant G glycoprotein region of the variant AsnN179  AspD  AlaA 205  Thr T. This result indicates that BRSV strains circulating among cattle can escape the host immune system without losing their pathogenicity and is considered to be one of the important factors that can cause respiratory system infection outbreaks in cattle.

 This study aimed to molecularly detect Bovine Respiratory Syncytial Virus BRSV in cattle with clinical signs of respiratory system infection and to conduct a phylogenetic analysis of the detected strains. To detect the pathogen, nasal swab samples collected from 96 cattle were subjected to Reverse Transcriptase  nested PCR using specific primer sets. As a result of the molecular techniques, viral nucleic acid was detected in 2.08 296 of the samples. Additionally, sequence and phylogenetic analyses of the detected local variant were performed and compared with various BRSV strains identified in different regions of Turkey and around the world. The genetic data obtained revealed that the isolates identified in this study belong to Subgroup III, alongside BRSV strains reported from various regions of Turkey, France, and the USA. Immunologically significant point mutations were detected in the immunodominant G glycoprotein region of the variant AsnN179  AspD  AlaA 205  Thr T. This result indicates that BRSV strains circulating among cattle can escape the host immune system without losing their pathogenicity and is considered to be one of the important factors that can cause respiratory system infection outbreaks in cattle.

 This study aimed to molecularly detect Bovine Respiratory Syncytial Virus BRSV in cattle with clinical signs of respiratory system infection and to conduct a phylogenetic analysis of the detected strains. To detect the pathogen, nasal swab samples collected from 96 cattle were subjected to Reverse Transcriptase  nested PCR using specific primer sets. As a result of the molecular techniques, viral nucleic acid was detected in 2.08 296 of the samples. Additionally, sequence and phylogenetic analyses of the detected local variant were performed and compared with various BRSV strains identified in different regions of Turkey and around the world. The genetic data obtained revealed that the isolates identified in this study belong to Subgroup III, alongside BRSV strains reported from various regions of Turkey, France, and the USA. Immunologically significant point mutations were detected in the immunodominant G glycoprotein region of the variant AsnN179  AspD  AlaA 205  Thr T. This result indicates that BRSV strains circulating among cattle can escape the host immune system without losing their pathogenicity and is considered to be one of the important factors that can cause respiratory system infection outbreaks in cattle.

 Bu araştırmada solunum sistemi enfeksiyonu klinik bulguları gösteren sığırlarda Bovine Respiratory Syncytial Virus BRSV un moleküler tespiti ve tespit edilen lokal suşların filogenetik analizlerinin gerçekleştirilmesi hedeflenmiştir. Etken tespiti için 96 sığırdan toplanan nazal sürüntü örneklerine spesifik primer setleri kullanılarak Reverz Transkriptaz-nested PCR RT-nPCR yöntemi uygulanmıştır. Uygulanan moleküler teknikler sonucunda  2.08 296 oranında viral nükleik asit tespiti gerçekleştirildi. Bununla birlikte tespit edilen lokal suşların sekans ve filogenetik analizleri gerçekleştirilerek Türkiye ve Dünyanın farklı bölgelerinden tespit edilmiş farklı BRSV suşları ile karşılaştırmaları yapıldı. Elde edilen genetik verilerden, bu çalışmada tespit edilen izolatların, Türkiye, Fransa ve ABD nin farklı bölgelerinden bildirilen BRSV suşları ile birlikte, Subgrup III içerisinde yer aldığı belirlenmiştir. Ayrıca izolatların immünodominant G glikoprotein bölgesinde immünolojik açıdan önemli nokta mutasyonları tespit edilmiştir AsnN179  AspD  AlaA 205  Thr T. Bu sonuç, sığırlar arasında dolaşan BRSV suşlarının patojenitelerini kaybetmeden konak bağışıklık sisteminden kaçabildiklerini ve sığırlarda solunum sistemi enfeksiyon salgınlarına neden olabilecek önemli faktörlerden biri olarak değerlendirilmiştir.

Makale detayları

Phylogenetic Analysis and Genetic Characterization of Bovine Respiratory Syncytial Virus G Glycoprotein / Bovine Respiratory Syncytial Virus G Glycoproteininin Filogenetik ve Analizi ve Genetik Karakterizasyonu

Yazarlar:

Ali Küçük | Özge Sevinç Korkmaz Akar | Yakup Yıldırım | Kamil Atlı

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Veteriner Viroloji

Dergi:

Antakya Veteriner Bilimleri Dergisi Vol.3 Issue 2, pp.32-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334275

pISSN:

2979-9805

“Cattle”

“BRSV”

“RT-nPCR”

“Genetic Characterization”“Cattle”

“BRSV”

“RT-nPCR”

“Genetic Characterization”

Sayı

Makale detayları

Yazarlar: Ali Küçük | Özge Sevinç Korkmaz Akar | Yakup Yıldırım | Kamil Atlı

Yayıncı: Hatay Mustafa Kemal University

Ana Başlıklar: Veteriner Viroloji

Dergi: Antakya Veteriner Bilimleri Dergisi Vol.3 Issue 2, pp.32-39

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001334275

pISSN: 2979-9805

Sayı

Yazarlar:

Ali Küçük | Özge Sevinç Korkmaz Akar | Yakup Yıldırım | Kamil Atlı

Yayıncı:

Hatay Mustafa Kemal University

Ana Başlıklar:

Veteriner Viroloji

Dergi:

Antakya Veteriner Bilimleri Dergisi Vol.3 Issue 2, pp.32-39

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334275

pISSN:

2979-9805