Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells

Ecem Kaya Sezginer; Omer Faruk Kırlangıç; Cengiz Karakaya; Sema Ören; Tuğba Fatsa; Taner Özgürtaş

Makale detayları

Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells

Yazarlar:

Ecem Kaya Sezginer | Omer Faruk Kırlangıç | Cengiz Karakaya | Sema Ören | Tuğba Fatsa | Taner Özgürtaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 1, pp.329-338

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345976

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 This study investigated the apoptotic effects of a histone deacetylase HDAC inhibitor, sodium butyrate NaBu and cisplatin as monotherapy or in combination on SH-SY5Y human neuroblastoma cells. MTT and colony formation assays were performed to investigate the anti-proliferative effects of NaBu in combination with cisplatin. Analysis of SH-SY5Y cell apoptosis was performed by Annexin V-FITCPI and DAPI staining. The gene and protein expressions were measured by RT-PCR and Western blot. The cytotoxic effect of the combined treatment was higher than single treatments, while the combination of two did not show much higher inhibition of colony formation and induction of apoptosis compared to cisplatin alone. Treatment with cisplatin upregulated the expression of Bax and Bcl-2 and combination treatment enhanced Bax gene expression and BaxBcl-2 ratio. NaBu alone and combined with cisplatin enhanced the expression of nuclear factor kappa B NF-κB protein, however cisplatin alone significantly decreased NFκB protein levels. p53 and phosphorylated inhibitor kappa B alpha p-IκBα expression did not alter following any of the treatments. The combination of NaBu with cisplatin did not show any additional benefit in SH-SY5Y human neuroblastoma cells compared to cisplatin as a conventional chemotherapeutic agent, probably due to the upregulation of antiapoptotic transcription factor NF-κB.

 This study investigated the apoptotic effects of a histone deacetylase HDAC inhibitor, sodium butyrate NaBu and cisplatin as monotherapy or in combination on SH-SY5Y human neuroblastoma cells. MTT and colony formation assays were performed to investigate the anti-proliferative effects of NaBu in combination with cisplatin. Analysis of SH-SY5Y cell apoptosis was performed by Annexin V-FITCPI and DAPI staining. The gene and protein expressions were measured by RT-PCR and Western blot. The cytotoxic effect of the combined treatment was higher than single treatments, while the combination of two did not show much higher inhibition of colony formation and induction of apoptosis compared to cisplatin alone. Treatment with cisplatin upregulated the expression of Bax and Bcl-2 and combination treatment enhanced Bax gene expression and BaxBcl-2 ratio. NaBu alone and combined with cisplatin enhanced the expression of nuclear factor kappa B NF-κB protein, however cisplatin alone significantly decreased NFκB protein levels. p53 and phosphorylated inhibitor kappa B alpha p-IκBα expression did not alter following any of the treatments. The combination of NaBu with cisplatin did not show any additional benefit in SH-SY5Y human neuroblastoma cells compared to cisplatin as a conventional chemotherapeutic agent, probably due to the upregulation of antiapoptotic transcription factor NF-κB.

 This study investigated the apoptotic effects of a histone deacetylase HDAC inhibitor, sodium butyrate NaBu and cisplatin as monotherapy or in combination on SH-SY5Y human neuroblastoma cells. MTT and colony formation assays were performed to investigate the anti-proliferative effects of NaBu in combination with cisplatin. Analysis of SH-SY5Y cell apoptosis was performed by Annexin V-FITCPI and DAPI staining. The gene and protein expressions were measured by RT-PCR and Western blot. The cytotoxic effect of the combined treatment was higher than single treatments, while the combination of two did not show much higher inhibition of colony formation and induction of apoptosis compared to cisplatin alone. Treatment with cisplatin upregulated the expression of Bax and Bcl-2 and combination treatment enhanced Bax gene expression and BaxBcl-2 ratio. NaBu alone and combined with cisplatin enhanced the expression of nuclear factor kappa B NF-κB protein, however cisplatin alone significantly decreased NFκB protein levels. p53 and phosphorylated inhibitor kappa B alpha p-IκBα expression did not alter following any of the treatments. The combination of NaBu with cisplatin did not show any additional benefit in SH-SY5Y human neuroblastoma cells compared to cisplatin as a conventional chemotherapeutic agent, probably due to the upregulation of antiapoptotic transcription factor NF-κB.

 This study investigated the apoptotic effects of a histone deacetylase HDAC inhibitor, sodium butyrate NaBu and cisplatin as monotherapy or in combination on SH-SY5Y human neuroblastoma cells. MTT and colony formation assays were performed to investigate the anti-proliferative effects of NaBu in combination with cisplatin. Analysis of SH-SY5Y cell apoptosis was performed by Annexin V-FITCPI and DAPI staining. The gene and protein expressions were measured by RT-PCR and Western blot. The cytotoxic effect of the combined treatment was higher than single treatments, while the combination of two did not show much higher inhibition of colony formation and induction of apoptosis compared to cisplatin alone. Treatment with cisplatin upregulated the expression of Bax and Bcl-2 and combination treatment enhanced Bax gene expression and BaxBcl-2 ratio. NaBu alone and combined with cisplatin enhanced the expression of nuclear factor kappa B NF-κB protein, however cisplatin alone significantly decreased NFκB protein levels. p53 and phosphorylated inhibitor kappa B alpha p-IκBα expression did not alter following any of the treatments. The combination of NaBu with cisplatin did not show any additional benefit in SH-SY5Y human neuroblastoma cells compared to cisplatin as a conventional chemotherapeutic agent, probably due to the upregulation of antiapoptotic transcription factor NF-κB.

Makale detayları

Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells

Yazarlar:

Ecem Kaya Sezginer | Omer Faruk Kırlangıç | Cengiz Karakaya | Sema Ören | Tuğba Fatsa | Taner Özgürtaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 1, pp.329-338

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345976

pISSN:

2630-6344

Histone deacetylase inhibitor

sodium butyrate

cisplatin

neuroblastoma; SH-SY5Y

Sayı

Makale detayları

Apoptosis inducing effect of sodium butyrate and cisplatin alone or in combination on SH-SY5Y human neuroblastoma cells

Yazarlar: Ecem Kaya Sezginer | Omer Faruk Kırlangıç | Cengiz Karakaya | Sema Ören | Tuğba Fatsa | Taner Özgürtaş

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Biochemistry

Dergi: Journal of Research in Pharmacy Vol.27 Issue 1, pp.329-338

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345976

pISSN: 2630-6344

Histone deacetylase inhibitor

sodium butyrate

cisplatin

neuroblastoma; SH-SY5Y

Sayı

Yazarlar:

Ecem Kaya Sezginer | Omer Faruk Kırlangıç | Cengiz Karakaya | Sema Ören | Tuğba Fatsa | Taner Özgürtaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 1, pp.329-338

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345976

pISSN:

2630-6344