Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos

Unsal Veli USTUNDAG; İsmail UNAL; Perihan Seda ATES; Ahmet Ata ALTURFAN; Turkan YİGİTBASİ; Ebru EMEKLİ ALTURFAN

doi:https://doi.org/10.5152/EurJBiol.2017.1705

Makale detayları

Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos

Yazarlar:

Unsal Veli USTUNDAG | İsmail UNAL | Perihan Seda ATES | Ahmet Ata ALTURFAN | Turkan YİGİTBASİ | Ebru EMEKLİ ALTURFAN

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2017 Vol.76 Issue 1, pp.26-30

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000424505

eISSN:

2618-6144

pISSN:

2602-2575

DOI:

https://doi.org/10.5152/EurJBiol.2017.1705

Özet:

 


Di-(2-ethylhexyl)-phthalate
(DEHP) is used in polyethylene terephthalate plastics and bisphenol-A (BPA) is
used to make polycarbonate plastics. The health-concerning effects of DEHP and
BPA indicate their potential toxic effects. The Wnt/β-catenin signaling pathway is necessary for embryonic
development. The c-MYC proto-oncogene (MYC) has been identified as a Wnt/β-catenin target gene, and MYC is a transcription
factor that regulates the expression of many gene products related to cell
proliferation, growth, differentiation, and apoptosis. Activation and
overexpression of the MYC gene has been reported in many human cancers and
during tumorigenesis. The aim of this study was to investigate the relationship
between oxidant-antioxidant balance and c-myc in BPA- and DEHP-exposed
zebrafish embryos. The real-time-polymerase chain reaction was used to
determine c-myc expression. Lipid peroxidation (LPO), nitric oxide (NO) levels,
and superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities
were determined in homogenates prepared from pooled embryo samples. Increased
expression of c-myc and decreased GST activity were observed in the BPA and
DEHP groups. NO levels increased and SOD activity decreased in the BPA group,
whereas LPO increased in the DEHP group. Disruption of oxidant-antioxidant
balance in DEHP- and BPA-exposed zebrafish embryos was associated with
increased c-myc expression and may be an important mechanism for the toxic
effects of these chemicals. 

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Makale detayları

Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos

Yazarlar: Unsal Veli USTUNDAG | İsmail UNAL | Perihan Seda ATES | Ahmet Ata ALTURFAN | Turkan YİGİTBASİ | Ebru EMEKLİ ALTURFAN

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2017 Vol.76 Issue 1, pp.26-30

Belge Tipi: Makale

Yayın Tarihi: 12/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000424505

eISSN: 2618-6144

pISSN: 2602-2575

DOI: https://doi.org/10.5152/EurJBiol.2017.1705

Sayı

Yazarlar:

Unsal Veli USTUNDAG | İsmail UNAL | Perihan Seda ATES | Ahmet Ata ALTURFAN | Turkan YİGİTBASİ | Ebru EMEKLİ ALTURFAN

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2017 Vol.76 Issue 1, pp.26-30

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000424505

eISSN:

2618-6144

pISSN:

2602-2575

DOI:

https://doi.org/10.5152/EurJBiol.2017.1705