Aronia melanocarpa (Michaux) Elliot Fruit Juice Attenuates Acetaminophen-induced Hepatotoxicity on Larval Zebrafish Model

Çiğdem Bilgi; Gülçin Çakan Akdoğan

Makale detayları

Aronia melanocarpa (Michaux) Elliot Fruit Juice Attenuates Acetaminophen-induced Hepatotoxicity on Larval Zebrafish Model

Yazarlar:

Çiğdem Bilgi | Gülçin Çakan Akdoğan

Yayıncı:

Ankara University

Ana Başlıklar:

Plant Biochemistry

Dergi:

Journal of Agricultural Sciences Vol.30 Issue 3, pp.458-463

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001323439

pISSN:

1300-7580

Anahtar kelimeler:

Özet:

 Aronia melanocarpa Michaux Elliot chokeberry is a natural medicinal plant with a rich content of phenolic compounds such as procyanidins, anthocyanins, and phenolic acids. Chokeberry fruits are gaining worldwide popularity due to the strong bioactivities of their phenolic constituents, such as antioxidant, anti-inflammatory, anticancer, and liver-protective effects. In the present study, total phenolic, flavonoid, and anthocyanin contents of chokeberry juice were determined via the Folin-Ciocalteu method, a spectrophotometric method based on AlCl3 complexation, and pH differential method, respectively. Anthocyanin content was determined as 1.14 equivalent to cyanidin-3-glucoside, while phenolic and flavonoid contents were measured as 5060.87 and 331.03 mg per 100 g of freeze-dried juice equivalent to gallic acid and quercetin, respectively. The hepatoprotective effects of chokeberry fruit juice were evaluated using a zebrafish in vivo model for acetaminophen APAP-induced liver injury. Zebrafish is an emerging in vivo liver injury model that enables hepatoprotective bioactivity screening of samples on live organisms. The APAP-induced liver injury model was established by treating zebrafish larvae with 5 mM APAP from 2 days post fertilization dpf to 5 dpf. The hepatoprotective effect of chokeberry was evaluated via exposure to 1, 10, and 100 μgmL of fruit juice. While chokeberry fruit juice did not cause any toxicity up to 100 μgmL, it successfully reduced the injury induced by APAP when applied at 1 μgmL concentration. To our knowledge, this is the first report evaluating the hepatoprotective effects of chokeberry using zebrafish in vivo liver injury model.

 Aronia melanocarpa Michaux Elliot chokeberry is a natural medicinal plant with a rich content of phenolic compounds such as procyanidins, anthocyanins, and phenolic acids. Chokeberry fruits are gaining worldwide popularity due to the strong bioactivities of their phenolic constituents, such as antioxidant, anti-inflammatory, anticancer, and liver-protective effects. In the present study, total phenolic, flavonoid, and anthocyanin contents of chokeberry juice were determined via the Folin-Ciocalteu method, a spectrophotometric method based on AlCl3 complexation, and pH differential method, respectively. Anthocyanin content was determined as 1.14 equivalent to cyanidin-3-glucoside, while phenolic and flavonoid contents were measured as 5060.87 and 331.03 mg per 100 g of freeze-dried juice equivalent to gallic acid and quercetin, respectively. The hepatoprotective effects of chokeberry fruit juice were evaluated using a zebrafish in vivo model for acetaminophen APAP-induced liver injury. Zebrafish is an emerging in vivo liver injury model that enables hepatoprotective bioactivity screening of samples on live organisms. The APAP-induced liver injury model was established by treating zebrafish larvae with 5 mM APAP from 2 days post fertilization dpf to 5 dpf. The hepatoprotective effect of chokeberry was evaluated via exposure to 1, 10, and 100 μgmL of fruit juice. While chokeberry fruit juice did not cause any toxicity up to 100 μgmL, it successfully reduced the injury induced by APAP when applied at 1 μgmL concentration. To our knowledge, this is the first report evaluating the hepatoprotective effects of chokeberry using zebrafish in vivo liver injury model.

 Aronia melanocarpa Michaux Elliot chokeberry is a natural medicinal plant with a rich content of phenolic compounds such as procyanidins, anthocyanins, and phenolic acids. Chokeberry fruits are gaining worldwide popularity due to the strong bioactivities of their phenolic constituents, such as antioxidant, anti-inflammatory, anticancer, and liver-protective effects. In the present study, total phenolic, flavonoid, and anthocyanin contents of chokeberry juice were determined via the Folin-Ciocalteu method, a spectrophotometric method based on AlCl3 complexation, and pH differential method, respectively. Anthocyanin content was determined as 1.14 equivalent to cyanidin-3-glucoside, while phenolic and flavonoid contents were measured as 5060.87 and 331.03 mg per 100 g of freeze-dried juice equivalent to gallic acid and quercetin, respectively. The hepatoprotective effects of chokeberry fruit juice were evaluated using a zebrafish in vivo model for acetaminophen APAP-induced liver injury. Zebrafish is an emerging in vivo liver injury model that enables hepatoprotective bioactivity screening of samples on live organisms. The APAP-induced liver injury model was established by treating zebrafish larvae with 5 mM APAP from 2 days post fertilization dpf to 5 dpf. The hepatoprotective effect of chokeberry was evaluated via exposure to 1, 10, and 100 μgmL of fruit juice. While chokeberry fruit juice did not cause any toxicity up to 100 μgmL, it successfully reduced the injury induced by APAP when applied at 1 μgmL concentration. To our knowledge, this is the first report evaluating the hepatoprotective effects of chokeberry using zebrafish in vivo liver injury model.

 Aronia melanocarpa Michaux Elliot chokeberry is a natural medicinal plant with a rich content of phenolic compounds such as procyanidins, anthocyanins, and phenolic acids. Chokeberry fruits are gaining worldwide popularity due to the strong bioactivities of their phenolic constituents, such as antioxidant, anti-inflammatory, anticancer, and liver-protective effects. In the present study, total phenolic, flavonoid, and anthocyanin contents of chokeberry juice were determined via the Folin-Ciocalteu method, a spectrophotometric method based on AlCl3 complexation, and pH differential method, respectively. Anthocyanin content was determined as 1.14 equivalent to cyanidin-3-glucoside, while phenolic and flavonoid contents were measured as 5060.87 and 331.03 mg per 100 g of freeze-dried juice equivalent to gallic acid and quercetin, respectively. The hepatoprotective effects of chokeberry fruit juice were evaluated using a zebrafish in vivo model for acetaminophen APAP-induced liver injury. Zebrafish is an emerging in vivo liver injury model that enables hepatoprotective bioactivity screening of samples on live organisms. The APAP-induced liver injury model was established by treating zebrafish larvae with 5 mM APAP from 2 days post fertilization dpf to 5 dpf. The hepatoprotective effect of chokeberry was evaluated via exposure to 1, 10, and 100 μgmL of fruit juice. While chokeberry fruit juice did not cause any toxicity up to 100 μgmL, it successfully reduced the injury induced by APAP when applied at 1 μgmL concentration. To our knowledge, this is the first report evaluating the hepatoprotective effects of chokeberry using zebrafish in vivo liver injury model.

Makale detayları

Aronia melanocarpa (Michaux) Elliot Fruit Juice Attenuates Acetaminophen-induced Hepatotoxicity on Larval Zebrafish Model

Yazarlar:

Çiğdem Bilgi | Gülçin Çakan Akdoğan

Yayıncı:

Ankara University

Ana Başlıklar:

Plant Biochemistry

Dergi:

Journal of Agricultural Sciences Vol.30 Issue 3, pp.458-463

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001323439

pISSN:

1300-7580

Anthocyanin

aronia

chokeberry

hepatoprotective

zebrafish

Sayı

Makale detayları

Aronia melanocarpa (Michaux) Elliot Fruit Juice Attenuates Acetaminophen-induced Hepatotoxicity on Larval Zebrafish Model

Yazarlar: Çiğdem Bilgi | Gülçin Çakan Akdoğan

Yayıncı: Ankara University

Ana Başlıklar: Plant Biochemistry

Dergi: Journal of Agricultural Sciences Vol.30 Issue 3, pp.458-463

Belge Tipi: Makale

Yayın Tarihi: 7/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001323439

pISSN: 1300-7580

Anthocyanin

aronia

chokeberry

hepatoprotective

zebrafish

Sayı

Yazarlar:

Çiğdem Bilgi | Gülçin Çakan Akdoğan

Yayıncı:

Ankara University

Ana Başlıklar:

Plant Biochemistry

Dergi:

Journal of Agricultural Sciences Vol.30 Issue 3, pp.458-463

Belge Tipi:

Makale

Yayın Tarihi:

7/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001323439

pISSN:

1300-7580