Production and Characterization of Monoclonal Anti-Ovalbumin Antibodies

Mehmet Ziya DOYMAZ

Makale detayları

Production and Characterization of Monoclonal Anti-Ovalbumin Antibodies

Yazarlar:

Mehmet Ziya DOYMAZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.411-416

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757509

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

In this study, the results of anti-  ovalbumin (OVA) monoclonal antibody  production and characterization are reported.  Splenocytes from OVA-hyperimmune Balb/C  mouse were fused with F0 myeloma cells and  resulting hybridoma cells were selected in  hypoxanthine-aminopterine-thymidine  containing medium. A total of 37 HAT  resistant hybridomas were obtained. Upon  testing the hybridoma supernatants in OVA  and mouse immunoglobulin G specific  enzyme-linked immunosorbent assays, we  observed that two of the hybridomas secreted  OVA specific antibodies. The hybridomas  produced IgG class antibodies. The  hybridomas were grown and the supernatants  from the cultures were also tested in a  Western Blot assay. The results of ELISA were  confirmed with Western Blot assays. The  results indicate that the mAbs produced in this  study reacted with the continuous (non-  conformational) epitopes present on OVA.

 

In this study, the results of anti-  ovalbumin (OVA) monoclonal antibody  production and characterization are reported.  Splenocytes from OVA-hyperimmune Balb/C  mouse were fused with F0 myeloma cells and  resulting hybridoma cells were selected in  hypoxanthine-aminopterine-thymidine  containing medium. A total of 37 HAT  resistant hybridomas were obtained. Upon  testing the hybridoma supernatants in OVA  and mouse immunoglobulin G specific  enzyme-linked immunosorbent assays, we  observed that two of the hybridomas secreted  OVA specific antibodies. The hybridomas  produced IgG class antibodies. The  hybridomas were grown and the supernatants  from the cultures were also tested in a  Western Blot assay. The results of ELISA were  confirmed with Western Blot assays. The  results indicate that the mAbs produced in this  study reacted with the continuous (non-  conformational) epitopes present on OVA.

Makale detayları

Production and Characterization of Monoclonal Anti-Ovalbumin Antibodies

Yazarlar:

Mehmet Ziya DOYMAZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.411-416

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757509

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Ovalbumin

monoclonal antibody

hybridoma.

Sayı

Makale detayları

Production and Characterization of Monoclonal Anti-Ovalbumin Antibodies

Yazarlar: Mehmet Ziya DOYMAZ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.411-416

Belge Tipi: Makale

Yayın Tarihi: 4/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000757509

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Ovalbumin

monoclonal antibody

hybridoma.

Sayı

Yazarlar:

Mehmet Ziya DOYMAZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2000 Vol.30 Issue 5, pp.411-416

Belge Tipi:

Makale

Yayın Tarihi:

4/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000757509

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144