Expression Profile of Transcription Factor ELK-1 and ELK-1 Target Genes on Lymphoma-Leukemia Cell Lines

Duygu YASAR SIRIN; Hande AKALAN

Makale detayları

Expression Profile of Transcription Factor ELK-1 and ELK-1 Target Genes on Lymphoma-Leukemia Cell Lines

Yazarlar:

Duygu YASAR SIRIN | Hande AKALAN

Yayıncı:

Süleyman Demirel University

Dergi:

Süleyman Demirel University Journal of Natural and Applied Sciences 2018 Vol.22 Issue 2, pp.388-392

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000553938

Sıklık:

Üç Yıllık

eISSN:

1308-6529

Özet:

 


Prognostic molecular markers identified in leukemia are becoming increasingly important especially in risk stratification and to determine therapy. In this study, we investigate the role of ELK-1 transcription factor and its potential target genes in four cell lines; Daudi, Jurkat, K-562 and HL-60. To evaluate ELK-1, MCPIP, MCL-1, BCL-10, CEBPB and SRF genes expression profiles we have performed a Real-time PCR analysis on Daudi, Jurkat, K-562 and HL-60 cell lines. ELK-1 over expression concomitant with SRF overexpression was detected only in Daudi cell line while only SRF overexpression was detected in jurkat cells. Expression of MCPIP, MCL-1, BCL-10 and CEBPB genes were decreased in all cell lines. Protein levels or phosphorylation status of ELK-1, BCL-10, CEBPB, MCL-1, MCPIP and SRF, moreover, changes that may occur when ELK-1 continuous overexpression is provided or completely silenced in these cell lines have not been evaluated. These questions are suggestions for future investigations.

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Makale detayları

Expression Profile of Transcription Factor ELK-1 and ELK-1 Target Genes on Lymphoma-Leukemia Cell Lines

Yazarlar: Duygu YASAR SIRIN | Hande AKALAN

Yayıncı: Süleyman Demirel University

Dergi: Süleyman Demirel University Journal of Natural and Applied Sciences 2018 Vol.22 Issue 2, pp.388-392

Belge Tipi: Makale

Yayın Tarihi: 8/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000553938

Sıklık: Üç Yıllık

eISSN: 1308-6529

Sayı

Yazarlar:

Duygu YASAR SIRIN | Hande AKALAN

Yayıncı:

Süleyman Demirel University

Dergi:

Süleyman Demirel University Journal of Natural and Applied Sciences 2018 Vol.22 Issue 2, pp.388-392

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000553938

Sıklık:

Üç Yıllık

eISSN:

1308-6529