Effect of Different Explant Sources, Metatopolin and PEG on In Vitro Propagation of Terebinth (Pistacia terebinthus L.)

Ali Tunç; Sevil Sağlam Yılmaz

Makale detayları

Effect of Different Explant Sources, Metatopolin and PEG on In Vitro Propagation of Terebinth (Pistacia terebinthus L.)

Yazarlar:

Ali Tunç | Sevil Sağlam Yılmaz

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Bitki Biyoteknolojisi

Dergi:

Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi Vol.3 Issue 2, pp.200-212

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001307289

pISSN:

2791-9161

Anahtar kelimeler:

Özet:

 Terebinth Pistacia terebinthus L. plant, which is from the Anacardiaceae family, is used as a forage plant in the nutrition of small ruminants; as an industrial plant in the production of biodiesel for fuel oil, in the cosmetic industry, in the prevention of environmental pollutants; it has a wide range of uses in coffee making, in the food industry as nuts and food coloring, in the production of antibacterial and antioxidants, and as a medicinal and aromatic plant. However, terebinth, which is used in so many areas, can be affected by unconscious grazing and harvesting, global climate changes, etc. it is in danger of extinction for various reasons. Plant genetic resources that are in danger of extinction should be reproduced using both classical and biotechnological methods. In addition, since the terebinth plant grows wild in our country and has the potential to be transformed into pistachios by grafting, it is extremely important to research and develop propagation methods and ensure its production. In this study, firstly, terebinth seeds were tried to germinate both in vitro and in vivo. Then, the effect of Metatopolin and PEG polymer on in vitro regeneration were investigated with different explants of the plant shoot tip, node, leaf. The data obtained will shed light on new studies planned to be carried out both in the propagation and breeding studies of the terebinth plant and in the production of secondary metabolites.

 Terebinth Pistacia terebinthus L. plant, which is from the Anacardiaceae family, is used as a forage plant in the nutrition of small ruminants; as an industrial plant in the production of biodiesel for fuel oil, in the cosmetic industry, in the prevention of environmental pollutants; it has a wide range of uses in coffee making, in the food industry as nuts and food coloring, in the production of antibacterial and antioxidants, and as a medicinal and aromatic plant. However, terebinth, which is used in so many areas, can be affected by unconscious grazing and harvesting, global climate changes, etc. it is in danger of extinction for various reasons. Plant genetic resources that are in danger of extinction should be reproduced using both classical and biotechnological methods. In addition, since the terebinth plant grows wild in our country and has the potential to be transformed into pistachios by grafting, it is extremely important to research and develop propagation methods and ensure its production. In this study, firstly, terebinth seeds were tried to germinate both in vitro and in vivo. Then, the effect of Metatopolin and PEG polymer on in vitro regeneration were investigated with different explants of the plant shoot tip, node, leaf. The data obtained will shed light on new studies planned to be carried out both in the propagation and breeding studies of the terebinth plant and in the production of secondary metabolites.

 Terebinth Pistacia terebinthus L. plant, which is from the Anacardiaceae family, is used as a forage plant in the nutrition of small ruminants; as an industrial plant in the production of biodiesel for fuel oil, in the cosmetic industry, in the prevention of environmental pollutants; it has a wide range of uses in coffee making, in the food industry as nuts and food coloring, in the production of antibacterial and antioxidants, and as a medicinal and aromatic plant. However, terebinth, which is used in so many areas, can be affected by unconscious grazing and harvesting, global climate changes, etc. it is in danger of extinction for various reasons. Plant genetic resources that are in danger of extinction should be reproduced using both classical and biotechnological methods. In addition, since the terebinth plant grows wild in our country and has the potential to be transformed into pistachios by grafting, it is extremely important to research and develop propagation methods and ensure its production. In this study, firstly, terebinth seeds were tried to germinate both in vitro and in vivo. Then, the effect of Metatopolin and PEG polymer on in vitro regeneration were investigated with different explants of the plant shoot tip, node, leaf. The data obtained will shed light on new studies planned to be carried out both in the propagation and breeding studies of the terebinth plant and in the production of secondary metabolites.

 Anacardiaceae Sakızağacıgiller familyasından olan menengiç Pistacia terebinthus L. bitkisi küçükbaş hayvanların beslenmesinde, akaryakıt amaçlı biyodizel yapımında, kozmetik sanayinde, çevre kirleticilerinin önlenmesinde, kahve yapımında, kuruyemiş ve gıda boyası olarak gıda sanayinde, antibakteriyel ve antioksidan üretiminde tıbbi ve aromatik bitki olarak geniş bir kullanım alanına sahiptir. Ancak bu kadar çok alanda kullanıma sahip olan menengiç gerek bilinçsiz otlatma ve sökümler, gerekse küresel iklim değişiklikleri vb. sebeplerden dolayı yokolma tehlikesi altındadır. Yokolma tehlikesi altında olan bitki gen kaynaklarının gerek klasik yöntemlerle gerekse biyoteknolojik yöntemlerle çoğaltılması sağlanmalıdır. Ayrıca menengiç bitkisi ülkemizde yabani olarak yetişmekte olduğundan ve aşılanarak Antep fıstığına dönüştürülme potansiyeli olmasından dolayı çoğaltma yöntemlerinin araştırılması ve geliştirilerek üretiminin sağlanması son derece önemlidir. Bu çalışmada menengiç tohumlarının in vitro ve in vivo koşullarda çimlendirme denemeleri kurulmuş ve PEG polimerinin çimlenmeye etkisi araştırılmıştır. Bitkinin sürgün ucu, boğum ve yaprak eksplantlarından sürgün rejenerasyonu üzerine 2,4,5-T ve metatopolinin etkileri araştırılmıştır. Elde edilen veriler menengiç bitkisinin gerek çoğaltım ve ıslah çalışmalarına gerekse sekonder metabolitlerin üretimi amacıyla yapılması planlanan yeni çalışmalara ışık tutacaktır.

Makale detayları

Effect of Different Explant Sources, Metatopolin and PEG on In Vitro Propagation of Terebinth (Pistacia terebinthus L.)

Yazarlar:

Ali Tunç | Sevil Sağlam Yılmaz

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Bitki Biyoteknolojisi

Dergi:

Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi Vol.3 Issue 2, pp.200-212

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001307289

pISSN:

2791-9161

Terebinth

micropropagation

tissue cultureMenengiç

mikroçoğaltım

doku kültürü

Sayı

Makale detayları

Effect of Different Explant Sources, Metatopolin and PEG on In Vitro Propagation of Terebinth (Pistacia terebinthus L.)

Yazarlar: Ali Tunç | Sevil Sağlam Yılmaz

Yayıncı: Kirsehir Ahi Evran University

Ana Başlıklar: Bitki Biyoteknolojisi

Dergi: Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi Vol.3 Issue 2, pp.200-212

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001307289

pISSN: 2791-9161

Terebinth

micropropagation

tissue cultureMenengiç

mikroçoğaltım

doku kültürü

Sayı

Yazarlar:

Ali Tunç | Sevil Sağlam Yılmaz

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Bitki Biyoteknolojisi

Dergi:

Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi Vol.3 Issue 2, pp.200-212

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001307289

pISSN:

2791-9161