Hypoxia Induced Changes in the Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, and Antioxidative Response Systems in Zea mays Leaves

Rashid JAMEI; Reza HEIDARI; Jalil KHARA; Samad ZARE

Makale detayları

Hypoxia Induced Changes in the Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, and Antioxidative Response Systems in Zea mays Leaves

Yazarlar:

Rashid JAMEI | Reza HEIDARI | Jalil KHARA | Samad ZARE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2009 Vol.33 Issue 1, pp.45-52

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

İngilizce

Document ID (Org No.):

2000813249

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The effects of hypoxia on the activities of some enzymes of antioxidative, non- enzymatic scavenging system, membrane permeability, lipid peroxidation, and some reactive oxygen species (ROS) in leaves of Zea mays were investigated. Samples were taken 48, 96, 144, and 192 h after the start of hypoxia treatment. A 192 h hypoxia treatment resulted in a significant rise in membrane permeability, lipid peroxidation (malondialdehyde level), and the production of hydrogen peroxide (H2O2) and superoxide (O2-) in maize leaves. A short duration of hypoxia enhanced the activity of superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and glutathione reductase (GR; EC 1.6.4.2), while further hypoxia significantly decreased the enzyme activity but increased the content of reduced glutathione (GSH) and ascorbic acid (AsA). It was observed that the reduction in SOD activity was greater than that in GR and APX (H2O2 scavengers). Our results showed that O2- induced membrane damage and lipid peroxidation, and that excessive accumulation of O2- is due to the reduced activity of SOD under hypoxia.

 

The effects of hypoxia on the activities of some enzymes of antioxidative, non- enzymatic scavenging system, membrane permeability, lipid peroxidation, and some reactive oxygen species (ROS) in leaves of Zea mays were investigated. Samples were taken 48, 96, 144, and 192 h after the start of hypoxia treatment. A 192 h hypoxia treatment resulted in a significant rise in membrane permeability, lipid peroxidation (malondialdehyde level), and the production of hydrogen peroxide (H2O2) and superoxide (O2-) in maize leaves. A short duration of hypoxia enhanced the activity of superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and glutathione reductase (GR; EC 1.6.4.2), while further hypoxia significantly decreased the enzyme activity but increased the content of reduced glutathione (GSH) and ascorbic acid (AsA). It was observed that the reduction in SOD activity was greater than that in GR and APX (H2O2 scavengers). Our results showed that O2- induced membrane damage and lipid peroxidation, and that excessive accumulation of O2- is due to the reduced activity of SOD under hypoxia.

Makale detayları

Hypoxia Induced Changes in the Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, and Antioxidative Response Systems in Zea mays Leaves

Yazarlar:

Rashid JAMEI | Reza HEIDARI | Jalil KHARA | Samad ZARE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2009 Vol.33 Issue 1, pp.45-52

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

İngilizce

Document ID (Org No.):

2000813249

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Antioxidant enzymes

ascorbate

glutathione

hypoxia

malondialdehyde

membrane permeability

reactive oxygen species

Zea mays

Sayı

Makale detayları

Yazarlar: Rashid JAMEI | Reza HEIDARI | Jalil KHARA | Samad ZARE

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2009 Vol.33 Issue 1, pp.45-52

Belge Tipi: Makale

Yayın Tarihi: 12/2008

Belge Dili: İngilizce

Document ID (Org No.): 2000813249

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Rashid JAMEI | Reza HEIDARI | Jalil KHARA | Samad ZARE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2009 Vol.33 Issue 1, pp.45-52

Belge Tipi:

Makale

Yayın Tarihi:

12/2008

Belge Dili:

İngilizce

Document ID (Org No.):

2000813249

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152