N-Nitro-L-Arginine Methyl Ester Hydrochloride Induced Endothelial Dysfunction and Atherosclerosis Model in Rats

Muhammet Kürşat Şimşek; Mustafa Mahmut Barış; Osman Yılmaz; Zekiye Altun; Safiye Aktaş; Yasemin Cakir; Sibel Büyükçoban; Mustafa Seçil

Makale detayları

N-Nitro-L-Arginine Methyl Ester Hydrochloride Induced Endothelial Dysfunction and Atherosclerosis Model in Rats

Yazarlar:

Muhammet Kürşat Şimşek | Mustafa Mahmut Barış | Osman Yılmaz | Zekiye Altun | Safiye Aktaş | Yasemin Cakir | Sibel Büyükçoban | Mustafa Seçil

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.114-121

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299975

pISSN:

2564-7288

Anahtar kelimeler:

Özet:

 Purpose: Atherosclerosis AS related diseases are the most common causes of mortality worldwide. N-Nitro-L-Arginine Methyl Ester Hydrochloride L-NAME-induced endothelial dysfunction ED and AS models require invasive methods for diagnosis. We aimed to establish noninvasive ultrasonography USG model for evaluating ED and AS in rats. Matherial and Methods: 23 Wistar Albino rats were divided into four groups. Right CCA rCCA, left CCA lCCA, abdominal aorta AA, and right iliac artery rIA IMT values of all rats were measured at the beginning of the experiment and before sacrification by USG. Right kidney RI values were calculated at similar times also. Histopathological and immunohistochemical analyzes were performed. In the sham group, rats received intraperitoneally IP sodium chloride. In the L-NAME groups, IP L-NAME was administered. Results: In the early effect group, significant increase was found in IMT measurements compared to the sham group. In the late effect group, significant increase was found in IMT measurements compared to the sham group. In addition, rRI increased significantly in the group at the end of the experiment. Conclusion: In small animal experiments which ED and AS were studied, a whole-body diagnostic noninvasive model was created for the first time with ultrasonography.

 Purpose: Atherosclerosis AS related diseases are the most common causes of mortality worldwide. N-Nitro-L-Arginine Methyl Ester Hydrochloride L-NAME-induced endothelial dysfunction ED and AS models require invasive methods for diagnosis. We aimed to establish noninvasive ultrasonography USG model for evaluating ED and AS in rats. Matherial and Methods: 23 Wistar Albino rats were divided into four groups. Right CCA rCCA, left CCA lCCA, abdominal aorta AA, and right iliac artery rIA IMT values of all rats were measured at the beginning of the experiment and before sacrification by USG. Right kidney RI values were calculated at similar times also. Histopathological and immunohistochemical analyzes were performed. In the sham group, rats received intraperitoneally IP sodium chloride. In the L-NAME groups, IP L-NAME was administered. Results: In the early effect group, significant increase was found in IMT measurements compared to the sham group. In the late effect group, significant increase was found in IMT measurements compared to the sham group. In addition, rRI increased significantly in the group at the end of the experiment. Conclusion: In small animal experiments which ED and AS were studied, a whole-body diagnostic noninvasive model was created for the first time with ultrasonography.

 Purpose: Atherosclerosis AS related diseases are the most common causes of mortality worldwide. N-Nitro-L-Arginine Methyl Ester Hydrochloride L-NAME-induced endothelial dysfunction ED and AS models require invasive methods for diagnosis. We aimed to establish noninvasive ultrasonography USG model for evaluating ED and AS in rats. Matherial and Methods: 23 Wistar Albino rats were divided into four groups. Right CCA rCCA, left CCA lCCA, abdominal aorta AA, and right iliac artery rIA IMT values of all rats were measured at the beginning of the experiment and before sacrification by USG. Right kidney RI values were calculated at similar times also. Histopathological and immunohistochemical analyzes were performed. In the sham group, rats received intraperitoneally IP sodium chloride. In the L-NAME groups, IP L-NAME was administered. Results: In the early effect group, significant increase was found in IMT measurements compared to the sham group. In the late effect group, significant increase was found in IMT measurements compared to the sham group. In addition, rRI increased significantly in the group at the end of the experiment. Conclusion: In small animal experiments which ED and AS were studied, a whole-body diagnostic noninvasive model was created for the first time with ultrasonography.

 Purpose: Atherosclerosis AS related diseases are the most common causes of mortality worldwide. N-Nitro-L-Arginine Methyl Ester Hydrochloride L-NAME-induced endothelial dysfunction ED and AS models require invasive methods for diagnosis. We aimed to establish noninvasive ultrasonography USG model for evaluating ED and AS in rats. Matherial and Methods: 23 Wistar Albino rats were divided into four groups. Right CCA rCCA, left CCA lCCA, abdominal aorta AA, and right iliac artery rIA IMT values of all rats were measured at the beginning of the experiment and before sacrification by USG. Right kidney RI values were calculated at similar times also. Histopathological and immunohistochemical analyzes were performed. In the sham group, rats received intraperitoneally IP sodium chloride. In the L-NAME groups, IP L-NAME was administered. Results: In the early effect group, significant increase was found in IMT measurements compared to the sham group. In the late effect group, significant increase was found in IMT measurements compared to the sham group. In addition, rRI increased significantly in the group at the end of the experiment. Conclusion: In small animal experiments which ED and AS were studied, a whole-body diagnostic noninvasive model was created for the first time with ultrasonography.

Makale detayları

N-Nitro-L-Arginine Methyl Ester Hydrochloride Induced Endothelial Dysfunction and Atherosclerosis Model in Rats

Yazarlar:

Muhammet Kürşat Şimşek | Mustafa Mahmut Barış | Osman Yılmaz | Zekiye Altun | Safiye Aktaş | Yasemin Cakir | Sibel Büyükçoban | Mustafa Seçil

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.114-121

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299975

pISSN:

2564-7288

Ultrasound

Intima-media tickness

L-NAME

renal resistive indexUltrasound

Intima-media tickness

L-NAME

renal resistive index

Sayı

Makale detayları

Yazarlar: Muhammet Kürşat Şimşek | Mustafa Mahmut Barış | Osman Yılmaz | Zekiye Altun | Safiye Aktaş | Yasemin Cakir | Sibel Büyükçoban | Mustafa Seçil

Yayıncı: Dokuz Eylul University

Ana Başlıklar: Health Care Administration

Dergi: Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.114-121

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001299975

pISSN: 2564-7288

Sayı

Yazarlar:

Muhammet Kürşat Şimşek | Mustafa Mahmut Barış | Osman Yılmaz | Zekiye Altun | Safiye Aktaş | Yasemin Cakir | Sibel Büyükçoban | Mustafa Seçil

Yayıncı:

Dokuz Eylul University

Ana Başlıklar:

Health Care Administration

Dergi:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 3, pp.114-121

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299975

pISSN:

2564-7288