Memantine activates LKB1-AMPK pathway in breast carcinoma cells

Elif Burcu BALİ

doi:https://doi.org/10.17776/csj.768907

Makale detayları

Memantine activates LKB1-AMPK pathway in breast carcinoma cells

Yazarlar:

Elif Burcu BALİ

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Cumhuriyet Science Journal 2020 Vol.41 Issue 4, pp.775-783

Belge Tipi:

Makale

Yayın Tarihi:

12/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000920339

DOI:

https://doi.org/10.17776/csj.768907

Özet:

 

Drug repositioning that is a screening of presently approved drugs for already unknown indications is therapeutically necessary and influential for drug discovery. In this study, it was aimed to research whether memantine as a repositioned drug can activate the LKB1-AMPK pathway in breast carcinoma cells by triggering tumor suppressor genes LKB1, AMPK, its downstream targets 40S ribosomal S6 kinases (S6K1 and S6K2), and eukaryotic initiation factor 4E-binding protein 4E-BP1. It was also evaluated its apoptotic effect by detecting the gene expressions of Caspase 7 and NOXA. Thus, MCF-7 cells were treated with 250 µM memantine for 48 h, and its cytotoxic effect was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. AMPKα1, AMPKα2, S6K1, S6K2, 4E-BP1, Caspase 7 and NOXA gene expression levels were measured by quantitative real-time polymerase chain reaction.  The results clearly revealed that memantine inhibited MCF-7 cell proliferation and activated the LKB1-AMPK pathway by reducing S6K1, S6K2, and 4EBP1 gene expressions. Memantine also augmented the gene expressions of Caspase 7 and NOXA. The findings reveal a molecular mechanism for the first time that may contribute to the anti-cancer effect of memantine to prevent or treat breast cancer. But further research should be performed to better understand its anti-cancer action. .

Makale detayları

Memantine activates LKB1-AMPK pathway in breast carcinoma cells

Yazarlar: Elif Burcu BALİ

Ana Başlıklar: Yaşam bilimleri

Dergi: Cumhuriyet Science Journal 2020 Vol.41 Issue 4, pp.775-783

Belge Tipi: Makale

Yayın Tarihi: 12/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000920339

DOI: https://doi.org/10.17776/csj.768907

Sayı

Yazarlar:

Elif Burcu BALİ

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Cumhuriyet Science Journal 2020 Vol.41 Issue 4, pp.775-783

Belge Tipi:

Makale

Yayın Tarihi:

12/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000920339

DOI:

https://doi.org/10.17776/csj.768907