Urginea  maritima (L.) Baker (Liliaceae) extract induces more cytotoxicity than standard chemotherapeutics in the A549 non-small cell lung cancer (NSCLC) cell line

Hakan BOZCUK; Mustafa ÖZDOĞAN; Oktay AYKURT; Fatih TOPÇUOĞLU; Hasan ÖZTÜRK; Deniz EKİNCİ; Asuman KARADENİZ; Aylin MUTLU; Durmuş BURGUCU

Makale detayları

Urginea maritima (L.) Baker (Liliaceae) extract induces more cytotoxicity than standard chemotherapeutics in the A549 non-small cell lung cancer (NSCLC) cell line

Yazarlar:

Hakan BOZCUK | Mustafa ÖZDOĞAN | Oktay AYKURT | Fatih TOPÇUOĞLU | Hasan ÖZTÜRK | Deniz EKİNCİ | Asuman KARADENİZ | Aylin MUTLU | Durmuş BURGUCU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 1, pp.101-108

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000754844

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Urginea maritima (Um) is a plant native to especially Turkey and the Mediterranean area. In this study, we investigated whether Um extract exerted cytotoxicity on cancer cells.  Materials and methods: We made various extracts of Um. These extracts in varying concentrations were added to an A549 NSCLC cell culture, alone or with gemcitabine and/or cisplatin. Growth inhibition was tested by the MTT assay and an Annexin V-FITC apoptosis detection kit   Results: The onion Um extract (1 µg/mL) was more cytotoxic than cisplatin (1 µg/mL), gemcitabine (1 nm/mL), and leaf Um extract (1 µg/mL) with P < 0.001, P = 0.097, and P < 0.001, respectively. The efficacy of Um extract was further improved by the addition of an antioxidant cocktail to an IC50 value of 0.02 µg/mL.  Conclusion: Um extract has been shown for the first time to be a strong candidate for drug development in solid tumors. Our studies continue to further define the specific antitumor compound(s) in this extract.

 

Urginea maritima (Um) is a plant native to especially Turkey and the Mediterranean area. In this study, we investigated whether Um extract exerted cytotoxicity on cancer cells.  Materials and methods: We made various extracts of Um. These extracts in varying concentrations were added to an A549 NSCLC cell culture, alone or with gemcitabine and/or cisplatin. Growth inhibition was tested by the MTT assay and an Annexin V-FITC apoptosis detection kit   Results: The onion Um extract (1 µg/mL) was more cytotoxic than cisplatin (1 µg/mL), gemcitabine (1 nm/mL), and leaf Um extract (1 µg/mL) with P < 0.001, P = 0.097, and P < 0.001, respectively. The efficacy of Um extract was further improved by the addition of an antioxidant cocktail to an IC50 value of 0.02 µg/mL.  Conclusion: Um extract has been shown for the first time to be a strong candidate for drug development in solid tumors. Our studies continue to further define the specific antitumor compound(s) in this extract.

Makale detayları

Urginea maritima (L.) Baker (Liliaceae) extract induces more cytotoxicity than standard chemotherapeutics in the A549 non-small cell lung cancer (NSCLC) cell line

Yazarlar:

Hakan BOZCUK | Mustafa ÖZDOĞAN | Oktay AYKURT | Fatih TOPÇUOĞLU | Hasan ÖZTÜRK | Deniz EKİNCİ | Asuman KARADENİZ | Aylin MUTLU | Durmuş BURGUCU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 1, pp.101-108

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000754844

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Urginea maritima

cytotoxicity

apoptosis

MTT

non-small cell lung cancer

Sayı

Makale detayları

Urginea maritima (L.) Baker (Liliaceae) extract induces more cytotoxicity than standard chemotherapeutics in the A549 non-small cell lung cancer (NSCLC) cell line

Yazarlar: Hakan BOZCUK | Mustafa ÖZDOĞAN | Oktay AYKURT | Fatih TOPÇUOĞLU | Hasan ÖZTÜRK | Deniz EKİNCİ | Asuman KARADENİZ | Aylin MUTLU | Durmuş BURGUCU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2011 Vol.41 Issue 1, pp.101-108

Belge Tipi: Makale

Yayın Tarihi: 12/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000754844

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Urginea maritima

cytotoxicity

apoptosis

MTT

non-small cell lung cancer

Sayı

Yazarlar:

Hakan BOZCUK | Mustafa ÖZDOĞAN | Oktay AYKURT | Fatih TOPÇUOĞLU | Hasan ÖZTÜRK | Deniz EKİNCİ | Asuman KARADENİZ | Aylin MUTLU | Durmuş BURGUCU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 1, pp.101-108

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000754844

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144