Determination of anticancer effects of Urospermum picroides against human cancer cell lines

Mehlika ALPER; Hatice GÜNEŞ

doi:https://doi.org/10.21448/ijsm.482404

Makale detayları

Determination of anticancer effects of Urospermum picroides against human cancer cell lines

Yazarlar:

Mehlika ALPER | Hatice GÜNEŞ

Yayıncı:

İzzet KARA

Ana Başlıklar:

Yaşam bilimleri

Dergi:

International Journal of Secondary Metabolite 2019 Vol.6 Issue 1, pp.28-37

Belge Tipi:

Makale

Yayın Tarihi:

3/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000471610

Sıklık:

Üç Aylık

eISSN:

2148-6905

DOI:

https://doi.org/10.21448/ijsm.482404

Özet:

 


Plants continue to be a good source for developing effective anticancer agents. In this study, in vitro various biological effects of crude ethanolic extract from flowering parts of Urospermum picroides collected from the Muğla province of Turkey were investigated for the first time. Daudi, A549 and HeLa cancer cell lines and BEAS-2B normal cell line were used to identify the cytotoxic effect of the extract using MTT assay. The effect of the extract on cell cycle progression was detected by flow cytometric analysis. The level of VEGF, IL-1α, IL-6 and TNF-α secretion in the cells treated with the extract were measured using ELISA The extract caused a higher cytotoxic effect on Daudi cells with an IC50 value of 85.64 µg/mL than the other cells tested. The IC50 values in HeLa and A549 cells were determined to be 135.35 and 234.8 µg/ mL, respectively. The selective cytotoxicity was considered between Daudi and BEAS-2B (109.80 µg/mL) cell lines. In addition, the effect of the extract on cell cycle progression changes according to cell line used. Moreover, the extract decreased the level of secreted VEGF in treated A549 cells by 31%. In addition, the extract resulted in a significant decrease in the secretion of IL-1α, IL-6 and TNF-α cytokines in A549 and Daudi cells compared to the untreated cells. These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.

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Makale detayları

Determination of anticancer effects of Urospermum picroides against human cancer cell lines

Yazarlar: Mehlika ALPER | Hatice GÜNEŞ

Yayıncı: İzzet KARA

Ana Başlıklar: Yaşam bilimleri

Dergi: International Journal of Secondary Metabolite 2019 Vol.6 Issue 1, pp.28-37

Belge Tipi: Makale

Yayın Tarihi: 3/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000471610

Sıklık: Üç Aylık

eISSN: 2148-6905

DOI: https://doi.org/10.21448/ijsm.482404

Sayı

Yazarlar:

Mehlika ALPER | Hatice GÜNEŞ

Yayıncı:

İzzet KARA

Ana Başlıklar:

Yaşam bilimleri

Dergi:

International Journal of Secondary Metabolite 2019 Vol.6 Issue 1, pp.28-37

Belge Tipi:

Makale

Yayın Tarihi:

3/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000471610

Sıklık:

Üç Aylık

eISSN:

2148-6905

DOI:

https://doi.org/10.21448/ijsm.482404