Anther Culture Potential of Linseed ( Linum usitatissimumL.):  Effects of Genotypes and Pretreatment on Callus  Formation and Differentiation

Orhan KURT

Makale detayları

Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation

Yazarlar:

Orhan KURT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 6, pp.553-560

Belge Tipi:

Makale

Yayın Tarihi:

5/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000810488

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid  and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus  induction rate both on solid and in liquid medium. The cultivar Blue-Chip gave, on average, the maximum (3.67%) callus induction  rate on solid medium and the cultivar Antares gave, on average, the maximum (3.35%) callus induction rate in liquid medium. The  cultivar Norman produced callus on solid medium but did not produce any callus in liquid medium. On the other hand, the cultivar  McGregor did not produce any callus in either solid or liquid medium. Pre-treatment of flower buds with cold significantly reduced  callus induction rate by about four fold compared with the no pre-treatment on solid medium but increased (not statistically  significant) callus induction rate by 25.8% in liquid medium. Although some callus induction was achieved in both on solid and in  liquid media, no differentiation was observed when calli were transferred to regeneration medium.

 

This study is concerned with the production of haploids of linseed. Anthers of eight genotypes were cultured both on solid  and in liquid medium. It was found that callus induction rate was low in both media and also the cultivars significantly affected callus  induction rate both on solid and in liquid medium. The cultivar Blue-Chip gave, on average, the maximum (3.67%) callus induction  rate on solid medium and the cultivar Antares gave, on average, the maximum (3.35%) callus induction rate in liquid medium. The  cultivar Norman produced callus on solid medium but did not produce any callus in liquid medium. On the other hand, the cultivar  McGregor did not produce any callus in either solid or liquid medium. Pre-treatment of flower buds with cold significantly reduced  callus induction rate by about four fold compared with the no pre-treatment on solid medium but increased (not statistically  significant) callus induction rate by 25.8% in liquid medium. Although some callus induction was achieved in both on solid and in  liquid media, no differentiation was observed when calli were transferred to regeneration medium.

Makale detayları

Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation

Yazarlar:

Orhan KURT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 6, pp.553-560

Belge Tipi:

Makale

Yayın Tarihi:

5/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000810488

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Turk. J. Agric. For.

22

(1998)

553-560. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.22

iss.6.

Sayı

Makale detayları

Anther Culture Potential of Linseed ( Linum usitatissimumL.): Effects of Genotypes and Pretreatment on Callus Formation and Differentiation

Yazarlar: Orhan KURT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 6, pp.553-560

Belge Tipi: Makale

Yayın Tarihi: 5/1998

Belge Dili: Türkçe

Document ID (Org No.): 2000810488

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Turk. J. Agric. For.

22

(1998)

553-560. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.22

iss.6.

Sayı

Yazarlar:

Orhan KURT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 6, pp.553-560

Belge Tipi:

Makale

Yayın Tarihi:

5/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000810488

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X