Antibiotic resistance of Escherichia coli isolates obtained from burn patients

Iman Alsarhan; Sedat Çam

Makale detayları

Antibiotic resistance of Escherichia coli isolates obtained from burn patients

Yazarlar:

Iman Alsarhan | Sedat Çam

Yayıncı:

Gumushane University

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 4, pp.780-789

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300899

pISSN:

2146-538X

Anahtar kelimeler:

Özet:

 Bacterial resistance to widely used antibiotics is an emerging global health issue and causes a huge problem in burn patients. Despite important developments in antimicrobial treatments, the risk of infection-associated mortality rate in burn patients is comparatively high. Escherichia coli is one of the most common causative agents of burn wound infections. Therefore, this study aimed to identify and characterize E. coli isolates from burn wounds using the VITEK 2 system and to test their antibiotic resistance to the most commonly used antibiotics with the disc diffusion method. In our study, of 147 clinical samples obtained from burn patients, 25 17 were detected as positive for E. coli. All these isolates were found to be resistant to cephalothin, cephradine, piperacillin, and rifampin antibiotics. The resistance to amoxicillinclavulanic acid and ampicillin was 96  8, which was followed by amikacin and cefotaxime with a 92  11 resistance rate. On the other hand, imipenem 96  8, tetracycline 88  13, and gentamicin 76  17 were the antibiotics that showed the highest sensitivity against E. coli isolates. The multidrug-resistant bacteria are one of the main issues for clinical applications, so their characterization is vital in developing a proper treatment strategy. This study concluded that E. coli exists in burn wounds and might cause wound infection due to its resistance to different antibiotics.

 Bacterial resistance to widely used antibiotics is an emerging global health issue and causes a huge problem in burn patients. Despite important developments in antimicrobial treatments, the risk of infection-associated mortality rate in burn patients is comparatively high. Escherichia coli is one of the most common causative agents of burn wound infections. Therefore, this study aimed to identify and characterize E. coli isolates from burn wounds using the VITEK 2 system and to test their antibiotic resistance to the most commonly used antibiotics with the disc diffusion method. In our study, of 147 clinical samples obtained from burn patients, 25 17 were detected as positive for E. coli. All these isolates were found to be resistant to cephalothin, cephradine, piperacillin, and rifampin antibiotics. The resistance to amoxicillinclavulanic acid and ampicillin was 96  8, which was followed by amikacin and cefotaxime with a 92  11 resistance rate. On the other hand, imipenem 96  8, tetracycline 88  13, and gentamicin 76  17 were the antibiotics that showed the highest sensitivity against E. coli isolates. The multidrug-resistant bacteria are one of the main issues for clinical applications, so their characterization is vital in developing a proper treatment strategy. This study concluded that E. coli exists in burn wounds and might cause wound infection due to its resistance to different antibiotics.

 Bacterial resistance to widely used antibiotics is an emerging global health issue and causes a huge problem in burn patients. Despite important developments in antimicrobial treatments, the risk of infection-associated mortality rate in burn patients is comparatively high. Escherichia coli is one of the most common causative agents of burn wound infections. Therefore, this study aimed to identify and characterize E. coli isolates from burn wounds using the VITEK 2 system and to test their antibiotic resistance to the most commonly used antibiotics with the disc diffusion method. In our study, of 147 clinical samples obtained from burn patients, 25 17 were detected as positive for E. coli. All these isolates were found to be resistant to cephalothin, cephradine, piperacillin, and rifampin antibiotics. The resistance to amoxicillinclavulanic acid and ampicillin was 96  8, which was followed by amikacin and cefotaxime with a 92  11 resistance rate. On the other hand, imipenem 96  8, tetracycline 88  13, and gentamicin 76  17 were the antibiotics that showed the highest sensitivity against E. coli isolates. The multidrug-resistant bacteria are one of the main issues for clinical applications, so their characterization is vital in developing a proper treatment strategy. This study concluded that E. coli exists in burn wounds and might cause wound infection due to its resistance to different antibiotics.

 Yaygın olarak kullanılan antibiyotiklere karşı bakteriyel direnç, gelişmekte olan küresel bir sağlık sorunudur ve yanık hastalarında büyük bir probleme neden olmaktadır. Antimikrobiyal tedavilerdeki önemli gelişmelere rağmen, yanık hastalarında enfeksiyona bağlı ölüm oranı riski nispeten yüksektir. Escherichia coli, yanık yarası enfeksiyonlarının en yaygın etkenlerinden biridir. Bu nedenle bu çalışmada yanık yaralarından E. coli izolatlarının VITEK 2 sistemi kullanılarak tanımlanması, karakterize edilmesi ve en sık kullanılan antibiyotiklere karşı antibiyotik dirençlerinin disk difüzyon yöntemi ile test edilmesi amaçlanmıştır. Çalışmamızda yanık hastalarından alınan 147 klinik örneğin 25inde 17 E. coli bakterisi saptandı. Tüm bu izolatların sefalotin, sefradin, piperasilin ve rifampin antibiyotiklere dirençli olduğu bulundu. Amoksisilinklavulanik asit ve ampisiline direnç 96  8, bunu 92  11 direnç oranıyla amikasin ve sefotaksim izledi. Öte yandan E. coli izolatlarına karşı en yüksek duyarlılığı gösteren antibiyotikler ise imipenem 96  8, tetrasiklin 88  13 ve gentamisin 76  17 olmuştur. Çoklu ilaca dirençli bakteriler, klinik uygulamalar için ana konulardan biridir, bu nedenle bunların karakterizasyonu, uygun bir tedavi stratejisi geliştirmede hayati önem taşır. Bu çalışma, E. colinin yanık yaralarında var olduğunu ve farklı antibiyotiklere direnci nedeniyle yara enfeksiyonuna neden olabileceği sonucuna varmıştır.

Makale detayları

Antibiotic resistance of Escherichia coli isolates obtained from burn patients

Yazarlar:

Iman Alsarhan | Sedat Çam

Yayıncı:

Gumushane University

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 4, pp.780-789

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300899

pISSN:

2146-538X

Antibiotic

Burn wound

Identification

VITEK 2Antibiyotik

Yanık yarası

Tanımlama

VITEK 2

Sayı

Makale detayları

Yazarlar: Iman Alsarhan | Sedat Çam

Yayıncı: Gumushane University

Ana Başlıklar: Mikrobiyoloji (Diğer)

Dergi: Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 4, pp.780-789

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001300899

pISSN: 2146-538X

Sayı

Yazarlar:

Iman Alsarhan | Sedat Çam

Yayıncı:

Gumushane University

Ana Başlıklar:

Mikrobiyoloji (Diğer)

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.13 Issue 4, pp.780-789

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300899

pISSN:

2146-538X