PROTECTIVE EFFECT OF RUTIN ON OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN VALPROAT-INDUCED GASTRIC TOXICITY

Sibel Çiğdem Tuncer; Cihan Gür; Nurhan Akaras; Fatih Mehmet Kandemir

Makale detayları

PROTECTIVE EFFECT OF RUTIN ON OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN VALPROAT-INDUCED GASTRIC TOXICITY

Yazarlar:

Sibel Çiğdem Tuncer | Cihan Gür | Nurhan Akaras | Fatih Mehmet Kandemir

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.334-342

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001299107

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective Sodium valproate is one of the most commonly used antiepileptic drugs and is toxic after long-term exposure. Long-term exposure to sodium valproate causes an increase in oxidative stress and inflammation in tissues. Rutin is a flavonoid naturally found in many plants with antioxidant, anti-inflammatory, and antiapoptotic effects. This study aimed to investigate the use and possible outcomes of rutin, a natural antioxidant, on gastric tissue damage caused by sodium valproate. Material and Method 35 Wistar albino rats were divided into 5 groups: control, rutin, sodium valproate, sodium valproaterutin-50mg, and sodium valproaterutin-100mg. For 14 days, a 500 mgkg dose of sodium valproate and 50 or 100 mgkg of rutin were administered by oral gavage. On the 15th day, rats were decapitated and gastric tissues were removed. SOD, CAT, GPx activities, MDA, GSH levels, and oxidative stress damage were analyzed by spectrophotometric method. Inflammation damage by NF-κB, TNF-α, COX-2, and MMP-9 transcription levels and apoptotic damage by Bax, Bcl-2, and Caspase-3 mRNA transcription levels were analyzed by RT-PCR method. Histologic analysis with hematoxylin-eosin staining was also performed to score for congestion, hemorrhage, mucosal damage, cell infiltration and gland dilatation. Results In gastric tissues, MDA level and NF-κB, TNF-α, COX- 2, MMP-9, Bax and Caspase-3 mRNA transcription levels increased p0.05, CAT, SOD, GPX activities, GSH level and Bcl-2 mRNA transcription level decreased p0.05 in sodium valproate group compared to control group. Rutin administration resulted in a reversal of all these changes due to sodium valproate p0.05. Conclusion It was concluded that rutin has potential protective properties against the toxic effect of sodium valproate exposure in gastric tissues.

 Objective Sodium valproate is one of the most commonly used antiepileptic drugs and is toxic after long-term exposure. Long-term exposure to sodium valproate causes an increase in oxidative stress and inflammation in tissues. Rutin is a flavonoid naturally found in many plants with antioxidant, anti-inflammatory, and antiapoptotic effects. This study aimed to investigate the use and possible outcomes of rutin, a natural antioxidant, on gastric tissue damage caused by sodium valproate. Material and Method 35 Wistar albino rats were divided into 5 groups: control, rutin, sodium valproate, sodium valproaterutin-50mg, and sodium valproaterutin-100mg. For 14 days, a 500 mgkg dose of sodium valproate and 50 or 100 mgkg of rutin were administered by oral gavage. On the 15th day, rats were decapitated and gastric tissues were removed. SOD, CAT, GPx activities, MDA, GSH levels, and oxidative stress damage were analyzed by spectrophotometric method. Inflammation damage by NF-κB, TNF-α, COX-2, and MMP-9 transcription levels and apoptotic damage by Bax, Bcl-2, and Caspase-3 mRNA transcription levels were analyzed by RT-PCR method. Histologic analysis with hematoxylin-eosin staining was also performed to score for congestion, hemorrhage, mucosal damage, cell infiltration and gland dilatation. Results In gastric tissues, MDA level and NF-κB, TNF-α, COX- 2, MMP-9, Bax and Caspase-3 mRNA transcription levels increased p0.05, CAT, SOD, GPX activities, GSH level and Bcl-2 mRNA transcription level decreased p0.05 in sodium valproate group compared to control group. Rutin administration resulted in a reversal of all these changes due to sodium valproate p0.05. Conclusion It was concluded that rutin has potential protective properties against the toxic effect of sodium valproate exposure in gastric tissues.

 Objective Sodium valproate is one of the most commonly used antiepileptic drugs and is toxic after long-term exposure. Long-term exposure to sodium valproate causes an increase in oxidative stress and inflammation in tissues. Rutin is a flavonoid naturally found in many plants with antioxidant, anti-inflammatory, and antiapoptotic effects. This study aimed to investigate the use and possible outcomes of rutin, a natural antioxidant, on gastric tissue damage caused by sodium valproate. Material and Method 35 Wistar albino rats were divided into 5 groups: control, rutin, sodium valproate, sodium valproaterutin-50mg, and sodium valproaterutin-100mg. For 14 days, a 500 mgkg dose of sodium valproate and 50 or 100 mgkg of rutin were administered by oral gavage. On the 15th day, rats were decapitated and gastric tissues were removed. SOD, CAT, GPx activities, MDA, GSH levels, and oxidative stress damage were analyzed by spectrophotometric method. Inflammation damage by NF-κB, TNF-α, COX-2, and MMP-9 transcription levels and apoptotic damage by Bax, Bcl-2, and Caspase-3 mRNA transcription levels were analyzed by RT-PCR method. Histologic analysis with hematoxylin-eosin staining was also performed to score for congestion, hemorrhage, mucosal damage, cell infiltration and gland dilatation. Results In gastric tissues, MDA level and NF-κB, TNF-α, COX- 2, MMP-9, Bax and Caspase-3 mRNA transcription levels increased p0.05, CAT, SOD, GPX activities, GSH level and Bcl-2 mRNA transcription level decreased p0.05 in sodium valproate group compared to control group. Rutin administration resulted in a reversal of all these changes due to sodium valproate p0.05. Conclusion It was concluded that rutin has potential protective properties against the toxic effect of sodium valproate exposure in gastric tissues.

 Amaç Sodyum valproat, antiepileptik ilaçlardan en yaygın kullanılanlardan birisi olup uzun süreli maruziyet sonucunda toksik etkilidir. Uzun süreli sodyum valproat maruziyeti dokularda özellikle oksidatif stres ve inflamasyon artışına neden olmaktadır. Rutin, birçok bitkide doğal olarak bulunan antioksidan, antiinflamatuvar ve antiapoptotik etkilere sahip bir flavanoiddir. Bu çalışmada, sodyum valproat kaynaklı mide doku hasarı üzerine doğal bir antioksidan olan rutinin kullanımı ve muhtemel etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntem 35 adet Wistar albino cinsi sıçan kontrol, rutin, sodyum valproat, sodyum valproatrutin-50mg ve sodyum valproatrutin-100mg grupları olmak üzere 5 gruba ayrıldı. 14 gün boyunca 500 mgkg dozda sodyum valproat uygulamasıyla birlikte 50 veya 100 mg kg rutin uygulaması oral gavaj yolla yapıldı. 15. günde sıçanlar dekapite edilerek mide dokuları alındı. SOD, KAT, GPx aktiviteleri ile MDA, GSH seviyeleri ile oksidatif stres hasarı spektrofotormetrik yöntem ile analiz edildi. NF-κB, TNF-α, COX-2 ve MMP-9 transkripsiyon düzeyleri ile inflamasyon hasarı ve Bax, Bcl-2, Kaspaz-3 mRNA transkripsiyon düzeyleri ile apoptotik hasar analizi RT-PCR yöntemi ile analiz edildi. Ayrıca konjesyon, hemoraji, mukoza hasarı, hücre infiltrasyonu ve bez dilatasyonu açısından skorlama için hematoksilen-eozin boyama ile histolojik analizler yapıldı. Bulgular Mide dokularında kontrol grubuna göre sodyum valproat grubunda MDA düzeyi ile NF-κB, TNF-α, MMP-9, COX-2, Bax ve Kaspaz-3 mRNA transkripsiyon düzeyleri artmış p0.05, KAT, SOD, GPX aktiviteleri ile GSH düzeyi ve Bcl-2 mRNA transkripsiyon düzeyi azalmıştır p0.05. Rutin uygulamasıyla birlikte sodyum valproata bağlı tüm bu değişikliklerde tersi yönde aktivite meydana gelmiştir p0.05. Sonuç Mide dokularında sodyum valproat maruziyetinin neden olduğu toksik etkiye karşı rutinin potansiyel koruyucu özelliklere sahip olduğu sonucuna varıldı.

Makale detayları

PROTECTIVE EFFECT OF RUTIN ON OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN VALPROAT-INDUCED GASTRIC TOXICITY

Yazarlar:

Sibel Çiğdem Tuncer | Cihan Gür | Nurhan Akaras | Fatih Mehmet Kandemir

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.334-342

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001299107

pISSN:

1300-7416

Valproate

Oxidative stress

Inflammation

Apoptosis

Gastric Toxicity

RutinApoptoz

İnflamasyon

Mide Toksisitesi

Oksidatif stres

Rutin

Valproat

Sayı

Makale detayları

Yazarlar: Sibel Çiğdem Tuncer | Cihan Gür | Nurhan Akaras | Fatih Mehmet Kandemir

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Clinical Sciences

Dergi: Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.334-342

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001299107

pISSN: 1300-7416

Sayı

Yazarlar:

Sibel Çiğdem Tuncer | Cihan Gür | Nurhan Akaras | Fatih Mehmet Kandemir

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 3, pp.334-342

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001299107

pISSN:

1300-7416