Determination of Bone Developments of Rat Anterior and Posterior Extremity Bones in Prenatal and Postnatal Period by Double Staining Method

Mustafa Öztürk; Erdoğan Unur; Niyazi Acer; Tolga Ertekin; Serife Alpa; Mesut Meker; Yahya Tahta

Makale detayları

Determination of Bone Developments of Rat Anterior and Posterior Extremity Bones in Prenatal and Postnatal Period by Double Staining Method

Yazarlar:

Mustafa Öztürk | Erdoğan Unur | Niyazi Acer | Tolga Ertekin | Serife Alpa | Mesut Meker | Yahya Tahta

Yayıncı:

Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Acta Medica Alanya Vol.7 Issue 2, pp.190-199

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001301820

pISSN:

2587-0319

Anahtar kelimeler:

Özet:

 Aim: In our study, we aimed to determine the morphological development of the bones of the anterior and posterior extremity by staining the rat fetus and offspring skeletons with the double staining method. Method: In the current study, seven groups three prenatal 16th, 18th, and 20th days and four postnatal 0th, 3th, 7th and 12th days were formed from the foetuses and offsprings obtained from 13 pregnant rats. Then, it was stained with double staining method. Anterior and posterior extremity images of the fetuses and offsprings were examined under a stereo microscope, and ossification findings were determined. Total bone and ossification lengths as well as ossification areas were measured using the ImageJ software. Results: The first cartilage destruction in fetuses occurred on the 16th day of pregnancy in the clavicle, scapula, humerus, radius and ulna; It was seen in the femur, tibia and fibula on the 18th day of pregnancy. The first ossification centres were in the clavicle, scapula and humerus on the 18th day of pregnancy; It was seen in the radius, ulna, femur, tibia, fibula and 2-5 metatarsal bones on the 20th day of pregnancy. The secondary ossification centre was seen on the 0th day birthday in the scapula and humerus, on the 7th day after birth in the ulna and radius, and on the 12th day after birth in the femur and tibia. In the study, while the ossification rate in 20-day-old foetuses was 48.9 in the humerus, 53.2 in the radius, 55.7 in the ulna, 33.6 in the femur, 43.2 in the tibia, 44.3 in the fibula, it was determined that it reached 69.7 in the humerus, 78.4 in the radius, 73.3 in the ulna, 63.5 in the femur, 75.5 in the tibia, and 69.2 in the fibula on the 12th day after birth. Conclusion: In this study, we revealed the morphological changes of the anterior and posterior extremity bones of fetuses and offsprings in the normal developmental course. We think that these results will shed light on the studies to be conducted on the detection of skeletal anomalies in teratological studies and contribute to a more comprehensive evaluation of the findings to be obtained from the studies to be conducted.

 Aim: In our study, we aimed to determine the morphological development of the bones of the anterior and posterior extremity by staining the rat fetus and offspring skeletons with the double staining method. Method: In the current study, seven groups three prenatal 16th, 18th, and 20th days and four postnatal 0th, 3th, 7th and 12th days were formed from the foetuses and offsprings obtained from 13 pregnant rats. Then, it was stained with double staining method. Anterior and posterior extremity images of the fetuses and offsprings were examined under a stereo microscope, and ossification findings were determined. Total bone and ossification lengths as well as ossification areas were measured using the ImageJ software. Results: The first cartilage destruction in fetuses occurred on the 16th day of pregnancy in the clavicle, scapula, humerus, radius and ulna; It was seen in the femur, tibia and fibula on the 18th day of pregnancy. The first ossification centres were in the clavicle, scapula and humerus on the 18th day of pregnancy; It was seen in the radius, ulna, femur, tibia, fibula and 2-5 metatarsal bones on the 20th day of pregnancy. The secondary ossification centre was seen on the 0th day birthday in the scapula and humerus, on the 7th day after birth in the ulna and radius, and on the 12th day after birth in the femur and tibia. In the study, while the ossification rate in 20-day-old foetuses was 48.9 in the humerus, 53.2 in the radius, 55.7 in the ulna, 33.6 in the femur, 43.2 in the tibia, 44.3 in the fibula, it was determined that it reached 69.7 in the humerus, 78.4 in the radius, 73.3 in the ulna, 63.5 in the femur, 75.5 in the tibia, and 69.2 in the fibula on the 12th day after birth. Conclusion: In this study, we revealed the morphological changes of the anterior and posterior extremity bones of fetuses and offsprings in the normal developmental course. We think that these results will shed light on the studies to be conducted on the detection of skeletal anomalies in teratological studies and contribute to a more comprehensive evaluation of the findings to be obtained from the studies to be conducted.

 Aim: In our study, we aimed to determine the morphological development of the bones of the anterior and posterior extremity by staining the rat fetus and offspring skeletons with the double staining method. Method: In the current study, seven groups three prenatal 16th, 18th, and 20th days and four postnatal 0th, 3th, 7th and 12th days were formed from the foetuses and offsprings obtained from 13 pregnant rats. Then, it was stained with double staining method. Anterior and posterior extremity images of the fetuses and offsprings were examined under a stereo microscope, and ossification findings were determined. Total bone and ossification lengths as well as ossification areas were measured using the ImageJ software. Results: The first cartilage destruction in fetuses occurred on the 16th day of pregnancy in the clavicle, scapula, humerus, radius and ulna; It was seen in the femur, tibia and fibula on the 18th day of pregnancy. The first ossification centres were in the clavicle, scapula and humerus on the 18th day of pregnancy; It was seen in the radius, ulna, femur, tibia, fibula and 2-5 metatarsal bones on the 20th day of pregnancy. The secondary ossification centre was seen on the 0th day birthday in the scapula and humerus, on the 7th day after birth in the ulna and radius, and on the 12th day after birth in the femur and tibia. In the study, while the ossification rate in 20-day-old foetuses was 48.9 in the humerus, 53.2 in the radius, 55.7 in the ulna, 33.6 in the femur, 43.2 in the tibia, 44.3 in the fibula, it was determined that it reached 69.7 in the humerus, 78.4 in the radius, 73.3 in the ulna, 63.5 in the femur, 75.5 in the tibia, and 69.2 in the fibula on the 12th day after birth. Conclusion: In this study, we revealed the morphological changes of the anterior and posterior extremity bones of fetuses and offsprings in the normal developmental course. We think that these results will shed light on the studies to be conducted on the detection of skeletal anomalies in teratological studies and contribute to a more comprehensive evaluation of the findings to be obtained from the studies to be conducted.

 Amaç: Çalışmamızda sıçan fetus ve yenidoğan iskeletinin ikili boyama yöntemi ile boyanarak, ön ve arka ekstremiteye ait kemiklerin morfolojik gelişimlerinin tespitini amaçladık. Yöntem: Bu çalışmada 13 adet gebe sıçandan elde edilen fetüs ve yavrulardan 3ü prenatal 16.,18. ve 20. gün ve 4ü postnatal 0, 3.,7. ve 12.gün olmak üzere 7 grup oluşturuldu. Daha sonra ikili boyama yöntemiyle boyandı. Fetüs ve yavruların ön ve arka ekstremite görüntüleri stereo mikroskop altında incelenerek ossifikasyon bulguları belirlendi. ImageJ yazılımı kullanılarak toplam kemik ve ossifikasyon uzunlukları ile ossifikasyon alanları ölçüldü. Bulgular: Fetüslerdeki ilk kıkırdak yıkımı clavicula, scapula, humerus, radius ve ulnada gebeliğin 16. gününde; femur, tibia ve fibulada ise gebeliğin 18. gününde görüldü. İlk kemikleşme merkezi clavicula, scapula ve humerusta gebeliğin 18. gününde; radius, ulna, femur, tibia, fibula ve 2-5 metatarsal kemiklerde gebeliğin 20. gününde görüldü. İkincil kemikleşme merkezi scapula ve humerusta 0. günde doğum günü, ulna ve radiusta doğumdan sonra 7. günde, femur ve tibiada doğumdan sonra 12. günde görüldü. Çalışmada 20 günlük fetuslerde kemikleşme oranı humerusta 48.9, radiusta 53.2, ulnada 55.7, femurda 33.6, tibiada 43.2, fibulada 44.3ken, doğumdan sonraki 12. günde humerusta 69.7, radiusta 78.4, ulnada 73.3, femurda 63.5, tibiada 75.5, fibulada 69.2e ulaştığı tespit edildi Sonuç: Bu çalışmada, fetüslere ve yenidoğan yavrulara ait ön ve arka ekstremite kemiklerinin normal gelişim seyrindeki morfolojik değişimlerini ortaya koyduk. Bu sonuçların teratolojik çalışmalarda iskelet anomalilerinin malformasyon, varyasyon ve diğer anomaliler tespitine yönelik yapılacak çalışmalara ışık tutacağını ve yapılacak çalışmalardan elde edilecek bulguların daha kapsamlı değerlendirilmesine katkı sunacağını düşünmekteyiz.

Makale detayları

Determination of Bone Developments of Rat Anterior and Posterior Extremity Bones in Prenatal and Postnatal Period by Double Staining Method

Yazarlar:

Mustafa Öztürk | Erdoğan Unur | Niyazi Acer | Tolga Ertekin | Serife Alpa | Mesut Meker | Yahya Tahta

Yayıncı:

Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Acta Medica Alanya Vol.7 Issue 2, pp.190-199

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001301820

pISSN:

2587-0319

Rat

Double Staining

Ossification

Bone Development

Image J

Toxicology.Sıçan

İkili Boyama

Kemikleşme

Kemik Gelişimi

Image J

Toksikoloji.

Sayı

Makale detayları

Yazarlar: Mustafa Öztürk | Erdoğan Unur | Niyazi Acer | Tolga Ertekin | Serife Alpa | Mesut Meker | Yahya Tahta

Yayıncı: Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar: Klinik Tıp Bilimleri (Diğer)

Dergi: Acta Medica Alanya Vol.7 Issue 2, pp.190-199

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001301820

pISSN: 2587-0319

Sayı

Yazarlar:

Mustafa Öztürk | Erdoğan Unur | Niyazi Acer | Tolga Ertekin | Serife Alpa | Mesut Meker | Yahya Tahta

Yayıncı:

Alanya Alaaddin Keykubat Üniversitesi

Ana Başlıklar:

Klinik Tıp Bilimleri (Diğer)

Dergi:

Acta Medica Alanya Vol.7 Issue 2, pp.190-199

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001301820

pISSN:

2587-0319