Step-by-step optimization of the HILIC method for simultaneous determination of abacavir, lamivudine, and zidovudine from dosage form

Cem ERKMEN; Mehmet GÜMÜŞTAŞ; Sibel Ayşil ÖZKAN; Bengi USLU

Makale detayları

Step-by-step optimization of the HILIC method for simultaneous determination of abacavir, lamivudine, and zidovudine from dosage form

Yazarlar:

Cem ERKMEN | Mehmet GÜMÜŞTAŞ | Sibel Ayşil ÖZKAN | Bengi USLU

Yayıncı:

TUBITAK

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 6, pp.1597-1607

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725529

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Combined therapy for HIV treatment shows superior   efficacy in comparison to single therapy. Furthermore, the separation and   determination of the combined dosage forms hold a significant place in the   pharmaceutical industry. Not only reverse phase high-performance liquid   chromatography (HPLC) but also hydrophilic interaction liquid chromatography   (HILIC) can be used for these purposes. Contrary to conventional RP systems,   HILIC may be an alternative for the analysis of polar substances. The aim of   this study was the step-by-step development of a simple, rapid, and reliable   method for the simultaneous determination of antiviral compounds from their   marketed formulation (Trizivir). In order to achieve this goal, various   mobile phase systems, buffer types, and concentrations were prepared to   provide an appropriate separation. Different types of columns were tested to   find the best resolution and high efficiency for the studied compounds. The   proposed method provided a simple procedure for the simultaneous analysis of   abacavir, lamivudine, and zidovudine in their pharmaceutical preparation   within approximately 2 min using the conventional HPLC system. The developed   method was fully validated according to the International Council for   Harmonisation guidelines from the viewpoint of selectivity, sensitivity,   precision, accuracy, linearity, limit of detection, and quantification.

Makale detayları

Step-by-step optimization of the HILIC method for simultaneous determination of abacavir, lamivudine, and zidovudine from dosage form

Yazarlar: Cem ERKMEN | Mehmet GÜMÜŞTAŞ | Sibel Ayşil ÖZKAN | Bengi USLU

Yayıncı: TUBITAK

Ana Başlıklar: Uygulamalı fizik

Dergi: Turkish Journal of Chemistry 2019 Vol.43 Issue 6, pp.1597-1607

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000725529

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Cem ERKMEN | Mehmet GÜMÜŞTAŞ | Sibel Ayşil ÖZKAN | Bengi USLU

Yayıncı:

TUBITAK

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 6, pp.1597-1607

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725529

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527