Investigation of mode of action of DNA insecticides on the  basis of LdMNPV IAP-3 gene

VOLODYMYR V. OBEREMOK; PALMAH M. NYADAR

Makale detayları

Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP-3 gene

Yazarlar:

VOLODYMYR V. OBEREMOK | PALMAH M. NYADAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.258-264

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811977

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

This study investigates the mechanism of action of DNA insecticides, a novel preparation against gypsy moth (Lymantria dispar) based on the DNA fragments of the antiapoptotic gene of its nuclear polyhedrosis virus. In our experiments gypsy moth showed significant susceptibility to applied DNA insecticides on the basis of BIR and RING domains fragments of LdMNPV IAP-3, whereas common fruit fly and tobacco hornworm did not. PCR with the same fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers revealed parts of the genomes of gypsy moth, common fruit fly, and tobacco hornworm. Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers has an overlap with the corresponding part of the LdMNPV IAP-3 gene and Lymantria dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover of the latter by query, which allows assuming that we cloned a part of a gypsy moth antiapoptosis gene. This finding suggests that DNA insecticides might act through the mechanisms characteristic for blocking of posttranscriptional expression of gypsy moth antiapoptosis genes.

 

This study investigates the mechanism of action of DNA insecticides, a novel preparation against gypsy moth (Lymantria dispar) based on the DNA fragments of the antiapoptotic gene of its nuclear polyhedrosis virus. In our experiments gypsy moth showed significant susceptibility to applied DNA insecticides on the basis of BIR and RING domains fragments of LdMNPV IAP-3, whereas common fruit fly and tobacco hornworm did not. PCR with the same fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers revealed parts of the genomes of gypsy moth, common fruit fly, and tobacco hornworm. Part of the gypsy moth genome cloned with the fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers has an overlap with the corresponding part of the LdMNPV IAP-3 gene and Lymantria dispar IAP-1 mRNA for an inhibitor of apoptosis protein with the high cover of the latter by query, which allows assuming that we cloned a part of a gypsy moth antiapoptosis gene. This finding suggests that DNA insecticides might act through the mechanisms characteristic for blocking of posttranscriptional expression of gypsy moth antiapoptosis genes.

Makale detayları

Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP-3 gene

Yazarlar:

VOLODYMYR V. OBEREMOK | PALMAH M. NYADAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.258-264

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811977

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

LdMNPV IAP-3 gene

BIR and RING domains

posttranscriptional silencing

DNA insecticides

crop protection

Sayı

Makale detayları

Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP-3 gene

Yazarlar: VOLODYMYR V. OBEREMOK | PALMAH M. NYADAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.258-264

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811977

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

LdMNPV IAP-3 gene

BIR and RING domains

posttranscriptional silencing

DNA insecticides

crop protection

Sayı

Yazarlar:

VOLODYMYR V. OBEREMOK | PALMAH M. NYADAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.258-264

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811977

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152