Beraprost sodium, a prostacyclin (PGI) analogue, ameliorates lipopolysaccharide-induced cellular injury in lung alveolar epithelial cells

SİNAN VICIL; SUAT ERDOGAN

doi:https://doi.org/10.3906/sag-1401-108

Makale detayları

Beraprost sodium, a prostacyclin (PGI) analogue, ameliorates lipopolysaccharide-induced cellular injury in lung alveolar epithelial cells

Yazarlar:

SİNAN VICIL | SUAT ERDOGAN

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.284-290

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000752759

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

DOI:

https://doi.org/10.3906/sag-1401-108

Anahtar kelimeler:

Özet:

 

Human alveolar epithelial cells play a critical role in the pathogenesis of lung diseases. The objective of this study is to determine the contribution of beraprost sodium, a prostaglandin I_2 (PGI_2) analogue, to inflammatory and oxidative events in response to lipopolysaccharide (LPS) in airway epithelial cells.  Materials and methods: Human pulmonary alveolar epithelial cells (A549) were pretreated with 10 µM beraprost sodium 30 min before stimulation with 1 µg/mL LPS for 24 h. The cellular viability assessments were evaluated by quantitative MTT test. Catalase activity and glutathione and lipid peroxidation levels were determined using spectrophotometric techniques. mRNA expression analyses were performed by real-time qRT-PCR.   Results: The endotoxin induced a dose-dependent increase in proliferation of the cells, which was suppressed by the beraprost sodium treatment. LPS increased the expressions of TNF-\alpha and IL-1\beta genes by 8- and 2.5-fold, respectively. It also induced lipid peroxidation and depleted cellular antioxidant capacity. Pretreatments of the cells with beraprost sodium significantly reversed the inflammation and suppressed oxidative stress.  Conclusion: These findings suggest that beraprost sodium will provide a pivotal molecular basis for the design of new therapeutic strategies to cure endotoxin-induced lung injury, although additional comprehensive studies are still required.

 

Human alveolar epithelial cells play a critical role in the pathogenesis of lung diseases. The objective of this study is to determine the contribution of beraprost sodium, a prostaglandin I_2 (PGI_2) analogue, to inflammatory and oxidative events in response to lipopolysaccharide (LPS) in airway epithelial cells.  Materials and methods: Human pulmonary alveolar epithelial cells (A549) were pretreated with 10 µM beraprost sodium 30 min before stimulation with 1 µg/mL LPS for 24 h. The cellular viability assessments were evaluated by quantitative MTT test. Catalase activity and glutathione and lipid peroxidation levels were determined using spectrophotometric techniques. mRNA expression analyses were performed by real-time qRT-PCR.   Results: The endotoxin induced a dose-dependent increase in proliferation of the cells, which was suppressed by the beraprost sodium treatment. LPS increased the expressions of TNF-\alpha and IL-1\beta genes by 8- and 2.5-fold, respectively. It also induced lipid peroxidation and depleted cellular antioxidant capacity. Pretreatments of the cells with beraprost sodium significantly reversed the inflammation and suppressed oxidative stress.  Conclusion: These findings suggest that beraprost sodium will provide a pivotal molecular basis for the design of new therapeutic strategies to cure endotoxin-induced lung injury, although additional comprehensive studies are still required.

Makale detayları

Beraprost sodium, a prostacyclin (PGI) analogue, ameliorates lipopolysaccharide-induced cellular injury in lung alveolar epithelial cells

Yazarlar:

SİNAN VICIL | SUAT ERDOGAN

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.284-290

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000752759

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

DOI:

https://doi.org/10.3906/sag-1401-108

Lung

epithelial cells

beraprost sodium

lipopolysaccharide

inflammation

oxidative stress

Sayı

Makale detayları

Beraprost sodium, a prostacyclin (PGI) analogue, ameliorates lipopolysaccharide-induced cellular injury in lung alveolar epithelial cells

Yazarlar: SİNAN VICIL | SUAT ERDOGAN

Yayıncı: TUBITAK

Ana Başlıklar: Sağlık teşvikleri

Dergi: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.284-290

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000752759

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

DOI: https://doi.org/10.3906/sag-1401-108

Lung

epithelial cells

beraprost sodium

lipopolysaccharide

inflammation

oxidative stress

Sayı

Yazarlar:

SİNAN VICIL | SUAT ERDOGAN

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.284-290

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000752759

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

DOI:

https://doi.org/10.3906/sag-1401-108