Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua

Bita GHASEMI; Ramin HOSSEINI; Fatemeh Dehghan NAYERI

Makale detayları

Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua

Yazarlar:

Bita GHASEMI | Ramin HOSSEINI | Fatemeh Dehghan NAYERI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.769-777

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815683

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

The low artemisinin content in Artemisia has caused this compound to be expensive among medicines. Several attempts have been made to increase its production by altering the expression of different genes. However, no approach has been cost-effective. In this study, the expression levels of SQS and DBR2 genes were quantified by qRT-PCR, and artemisinin content was measured by HPLC in Artemisia annua cell suspension culture under nano cobalt particles elicitation. For this purpose, nano cobalt particles were used in 0.25, 2.5, and 5 mg L-1 concentrations and samples were collected after 8, 24, 48, and 72 h. The highest artemisinin content was observed 24 h after 5 mg L-1 nano cobalt treatment. In this case, artemisinin production was 2.25-fold (113.35 mg g-1 dw) higher than that of the control. Simultaneously, the expression levels of SQS and DBR2 genes decreased. It appears that the decrease in the expression of SQS and DBR2 genes caused the artemisinin content to increase by high concentrations of the nano cobalt particles.

 

The low artemisinin content in Artemisia has caused this compound to be expensive among medicines. Several attempts have been made to increase its production by altering the expression of different genes. However, no approach has been cost-effective. In this study, the expression levels of SQS and DBR2 genes were quantified by qRT-PCR, and artemisinin content was measured by HPLC in Artemisia annua cell suspension culture under nano cobalt particles elicitation. For this purpose, nano cobalt particles were used in 0.25, 2.5, and 5 mg L-1 concentrations and samples were collected after 8, 24, 48, and 72 h. The highest artemisinin content was observed 24 h after 5 mg L-1 nano cobalt treatment. In this case, artemisinin production was 2.25-fold (113.35 mg g-1 dw) higher than that of the control. Simultaneously, the expression levels of SQS and DBR2 genes decreased. It appears that the decrease in the expression of SQS and DBR2 genes caused the artemisinin content to increase by high concentrations of the nano cobalt particles.

Makale detayları

Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua

Yazarlar:

Bita GHASEMI | Ramin HOSSEINI | Fatemeh Dehghan NAYERI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.769-777

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815683

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Artemisinin

nano elicitor

cobalt

gene expression

HPLC

QRT-PCR

Sayı

Makale detayları

Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua

Yazarlar: Bita GHASEMI | Ramin HOSSEINI | Fatemeh Dehghan NAYERI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.769-777

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000815683

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Artemisinin

nano elicitor

cobalt

gene expression

HPLC

QRT-PCR

Sayı

Yazarlar:

Bita GHASEMI | Ramin HOSSEINI | Fatemeh Dehghan NAYERI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.769-777

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815683

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X