A Densitometric Method for Determination of Mangiferin, an Antioxidant Compound, with Thin Layer Chromatography in the Leaf Extracts of Coffee (Coffea Arabica L.)

Yuni Retnaningtyas; Nora Putri Narindra; Nia Kristiningrum

Makale detayları

A Densitometric Method for Determination of Mangiferin, an Antioxidant Compound, with Thin Layer Chromatography in the Leaf Extracts of Coffee (Coffea Arabica L.)

Yazarlar:

Yuni Retnaningtyas | Nora Putri Narindra | Nia Kristiningrum

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Physical Chemistry (Other)

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.331-340

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001303731

pISSN:

2149-0120

Anahtar kelimeler:

Özet:

 Mangiferin is one of the antioxidants in Coffea arabica L. leaves that has many pharmacological effects. The content of secondary metabolites in the leaves including mangiferin can be affected by age. A Thin Layer Chromatography TLC method for the quantitative analysis of mangiferin in Coffea arabica L. leaves extract was developed and validated. The method was developed using a mobile phase prepared with analytical grade solvents: ethyl acetate, methanol, formic acid, and deionized water 8:2:1:1 vvvv. Regression functions were established over the 199.98-600.00 ngspot range with r0.999. The limit of detection LOD and limit of quantification LOQ were 13.87 and 41.61 ng, respectively. The method was selective with a resolution value of more than 1.5 and specific with the spectra correlation value for purity and identity check of more than 0.99. The percentage RSD was found to be 2.43 for repeatability precision and 2.05 for intermediate precision. The methods accuracy was determined through the standard addition method by adding known quantities of standard mangiferin to the pre-analyzed test solution and the mean recovery was 101.69 1,21. This TLC Densitometry method was linear, sensitive, selective and specific, precise, accurate, and can be used for routine analysis of mangiferin. On the young Coffea arabica L. leaves, the concentration of mangiferin  RSD was 0.830  1.71 ww, and on the old Coffea arabica L. leaves was 1.128 1.59 ww.

 Mangiferin is one of the antioxidants in Coffea arabica L. leaves that has many pharmacological effects. The content of secondary metabolites in the leaves including mangiferin can be affected by age. A Thin Layer Chromatography TLC method for the quantitative analysis of mangiferin in Coffea arabica L. leaves extract was developed and validated. The method was developed using a mobile phase prepared with analytical grade solvents: ethyl acetate, methanol, formic acid, and deionized water 8:2:1:1 vvvv. Regression functions were established over the 199.98-600.00 ngspot range with r0.999. The limit of detection LOD and limit of quantification LOQ were 13.87 and 41.61 ng, respectively. The method was selective with a resolution value of more than 1.5 and specific with the spectra correlation value for purity and identity check of more than 0.99. The percentage RSD was found to be 2.43 for repeatability precision and 2.05 for intermediate precision. The methods accuracy was determined through the standard addition method by adding known quantities of standard mangiferin to the pre-analyzed test solution and the mean recovery was 101.69 1,21. This TLC Densitometry method was linear, sensitive, selective and specific, precise, accurate, and can be used for routine analysis of mangiferin. On the young Coffea arabica L. leaves, the concentration of mangiferin  RSD was 0.830  1.71 ww, and on the old Coffea arabica L. leaves was 1.128 1.59 ww.

 Mangiferin is one of the antioxidants in Coffea arabica L. leaves that has many pharmacological effects. The content of secondary metabolites in the leaves including mangiferin can be affected by age. A Thin Layer Chromatography TLC method for the quantitative analysis of mangiferin in Coffea arabica L. leaves extract was developed and validated. The method was developed using a mobile phase prepared with analytical grade solvents: ethyl acetate, methanol, formic acid, and deionized water 8:2:1:1 vvvv. Regression functions were established over the 199.98-600.00 ngspot range with r0.999. The limit of detection LOD and limit of quantification LOQ were 13.87 and 41.61 ng, respectively. The method was selective with a resolution value of more than 1.5 and specific with the spectra correlation value for purity and identity check of more than 0.99. The percentage RSD was found to be 2.43 for repeatability precision and 2.05 for intermediate precision. The methods accuracy was determined through the standard addition method by adding known quantities of standard mangiferin to the pre-analyzed test solution and the mean recovery was 101.69 1,21. This TLC Densitometry method was linear, sensitive, selective and specific, precise, accurate, and can be used for routine analysis of mangiferin. On the young Coffea arabica L. leaves, the concentration of mangiferin  RSD was 0.830  1.71 ww, and on the old Coffea arabica L. leaves was 1.128 1.59 ww.

 Mangiferin is one of the antioxidants in Coffea arabica L. leaves that has many pharmacological effects. The content of secondary metabolites in the leaves including mangiferin can be affected by age. A Thin Layer Chromatography TLC method for the quantitative analysis of mangiferin in Coffea arabica L. leaves extract was developed and validated. The method was developed using a mobile phase prepared with analytical grade solvents: ethyl acetate, methanol, formic acid, and deionized water 8:2:1:1 vvvv. Regression functions were established over the 199.98-600.00 ngspot range with r0.999. The limit of detection LOD and limit of quantification LOQ were 13.87 and 41.61 ng, respectively. The method was selective with a resolution value of more than 1.5 and specific with the spectra correlation value for purity and identity check of more than 0.99. The percentage RSD was found to be 2.43 for repeatability precision and 2.05 for intermediate precision. The methods accuracy was determined through the standard addition method by adding known quantities of standard mangiferin to the pre-analyzed test solution and the mean recovery was 101.69 1,21. This TLC Densitometry method was linear, sensitive, selective and specific, precise, accurate, and can be used for routine analysis of mangiferin. On the young Coffea arabica L. leaves, the concentration of mangiferin  RSD was 0.830  1.71 ww, and on the old Coffea arabica L. leaves was 1.128 1.59 ww.

Makale detayları

A Densitometric Method for Determination of Mangiferin, an Antioxidant Compound, with Thin Layer Chromatography in the Leaf Extracts of Coffee (Coffea Arabica L.)

Yazarlar:

Yuni Retnaningtyas | Nora Putri Narindra | Nia Kristiningrum

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Physical Chemistry (Other)

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.331-340

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001303731

pISSN:

2149-0120

Coffea robusta L.

leaves

mangiferin

validation

TLC-Densitometry

Sayı

Makale detayları

A Densitometric Method for Determination of Mangiferin, an Antioxidant Compound, with Thin Layer Chromatography in the Leaf Extracts of Coffee (Coffea Arabica L.)

Yazarlar: Yuni Retnaningtyas | Nora Putri Narindra | Nia Kristiningrum

Yayıncı: Turkish Chemical Society

Ana Başlıklar: Physical Chemistry (Other)

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.331-340

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001303731

pISSN: 2149-0120

Coffea robusta L.

leaves

mangiferin

validation

TLC-Densitometry

Sayı

Yazarlar:

Yuni Retnaningtyas | Nora Putri Narindra | Nia Kristiningrum

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Physical Chemistry (Other)

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 1, pp.331-340

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001303731

pISSN:

2149-0120