An Experimental Study on the Use of Exosomes against Acetaminophen-induced Uterine and Fallopian Tubes Damage in Rats

Ozge Goktepe

Makale detayları

An Experimental Study on the Use of Exosomes against Acetaminophen-induced Uterine and Fallopian Tubes Damage in Rats

Yazarlar:

Ozge Goktepe

Yayıncı:

Erciyes University

Ana Başlıklar:

Veteriner Doğum ve Jinekoloji | Veteriner Histoloji ve Embriyolojisi

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 2, pp.127-133

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293731

pISSN:

1304-7280

Anahtar kelimeler:

Özet:

 Acetaminophen APAP is an antipyretic and analgesic drug that can be bought and used without a prescrip- tion worldwide. A dosage of APAP greater than the maximum recommended dosage can increase the risk of organ damage. Mesenchymal stem cells MSCs are isolated from various human tissues and used for therapy, in which beneficial effects are attributed mainly to mesenchymal stem cell-derived extracellular vesicles MSC-EVs. However, no study has focused on the protective effect of exosomes in combination with APAP. Therefore, the present study was carried out to investigate the protective effect of taking exosomes against APAP toxicity on the fallopian tubes and uterus. Forty female Wistar albino rats 1214 weeks old were randomly divided into four equal groups: control, APAP received 1 gkg APAP, exosome received 30 μg of exosomes, and APAPexosome groups that received simultane- ously 1 gkg APAP and were followed three days later by a tail vein injection 30 μg of exosomes. The uterus and fallo- pian tubes were removed for histological and immunohistochemical analyses after the animals were sacrificed. The results showed that exosomes administration after APAP decreased APAPs autophagic effects. Moreover, exosome treatment exhibited a protective effect on the immunoreactivity intensities of autuphagy markers Beclin-1, p62, and LC3. The treatments with exosomes had no adverse effect on the uterus or fallopian tubes. The administration of exo- somes after APAP toxicity can decrease cell death through the autophagy effect of APAP. It is suggested that this com- pound can decrease the toxic effects of APAP. Further studies are needed to evaluate the molecular mechanism of this hyperanalgesic effect.

 Acetaminophen APAP is an antipyretic and analgesic drug that can be bought and used without a prescrip- tion worldwide. A dosage of APAP greater than the maximum recommended dosage can increase the risk of organ damage. Mesenchymal stem cells MSCs are isolated from various human tissues and used for therapy, in which beneficial effects are attributed mainly to mesenchymal stem cell-derived extracellular vesicles MSC-EVs. However, no study has focused on the protective effect of exosomes in combination with APAP. Therefore, the present study was carried out to investigate the protective effect of taking exosomes against APAP toxicity on the fallopian tubes and uterus. Forty female Wistar albino rats 1214 weeks old were randomly divided into four equal groups: control, APAP received 1 gkg APAP, exosome received 30 μg of exosomes, and APAPexosome groups that received simultane- ously 1 gkg APAP and were followed three days later by a tail vein injection 30 μg of exosomes. The uterus and fallo- pian tubes were removed for histological and immunohistochemical analyses after the animals were sacrificed. The results showed that exosomes administration after APAP decreased APAPs autophagic effects. Moreover, exosome treatment exhibited a protective effect on the immunoreactivity intensities of autuphagy markers Beclin-1, p62, and LC3. The treatments with exosomes had no adverse effect on the uterus or fallopian tubes. The administration of exo- somes after APAP toxicity can decrease cell death through the autophagy effect of APAP. It is suggested that this com- pound can decrease the toxic effects of APAP. Further studies are needed to evaluate the molecular mechanism of this hyperanalgesic effect.

 Acetaminophen APAP is an antipyretic and analgesic drug that can be bought and used without a prescrip- tion worldwide. A dosage of APAP greater than the maximum recommended dosage can increase the risk of organ damage. Mesenchymal stem cells MSCs are isolated from various human tissues and used for therapy, in which beneficial effects are attributed mainly to mesenchymal stem cell-derived extracellular vesicles MSC-EVs. However, no study has focused on the protective effect of exosomes in combination with APAP. Therefore, the present study was carried out to investigate the protective effect of taking exosomes against APAP toxicity on the fallopian tubes and uterus. Forty female Wistar albino rats 1214 weeks old were randomly divided into four equal groups: control, APAP received 1 gkg APAP, exosome received 30 μg of exosomes, and APAPexosome groups that received simultane- ously 1 gkg APAP and were followed three days later by a tail vein injection 30 μg of exosomes. The uterus and fallo- pian tubes were removed for histological and immunohistochemical analyses after the animals were sacrificed. The results showed that exosomes administration after APAP decreased APAPs autophagic effects. Moreover, exosome treatment exhibited a protective effect on the immunoreactivity intensities of autuphagy markers Beclin-1, p62, and LC3. The treatments with exosomes had no adverse effect on the uterus or fallopian tubes. The administration of exo- somes after APAP toxicity can decrease cell death through the autophagy effect of APAP. It is suggested that this com- pound can decrease the toxic effects of APAP. Further studies are needed to evaluate the molecular mechanism of this hyperanalgesic effect.

 Asetaminofen APAP, dünya çapında reçetesiz satın alınabilen ve kullanılabilen bir antipiretik ve analjezik ilaçtır. Önerilen maksimum dozdan daha yüksek bir APAP dozu, organ hasarı riskini artırabilir. Mezenkimal kök hücreler MSCler, çeşitli insan dokularından izole edilir ve yararlı etkilerin esas olarak mezenkimal kök hücreden türetilen hüc- re dışı veziküllere MSC-EVler atfedildiği terapi için kullanılır. Bununla birlikte, hiçbir çalışma eksozomların APAP ile kombinasyon halinde koruyucu etkisine odaklanmamıştır. Bu nedenle bu çalışma APAP toksisitesine karşı eksozom almanın fallop tüpleri ve uterus üzerindeki koruyucu etkisini araştırmak amacıyla yapılmıştır. Kırk dişi Wistar albino sıçan 12-14 haftalık rastgele dört eşit gruba ayrıldı: kontrol, APAP 1 gkg APAP aldı, eksozom 30 μg eksozom aldı ve APAPeksozom grupları eş zamanlı olarak 1 gkg APAP ve ardından üç gün sonra kuyruk damarından 30 ug ekso- zom enjeksiyonu yapıldı. Hayvanlar sakrifiye edildikten sonra histolojik ve immünohistokimyasal analizler için uterus ve fallop tüpleri çıkarıldı. Sonuçlar, eksozomların APAPtan sonra uygulanmasının APAPın otofajik etkilerini azalttığını gösterdi. Ayrıca, eksozom tedavisi, otofaji belirteçlerinin Beclin-1, p62 ve LC3 immünoreaktivite yoğunlukları üzerinde koruyucu bir etki sergiledi. Eksozomlarla yapılan tedavilerin uterus veya fallop tüpleri üzerinde herhangi bir olumsuz etkisi olmamıştır. APAP toksisitesinden sonra eksozomların uygulanması, APAPın otofaji etkisi yoluyla hücre ölümünü azaltabilir. Bu bileşiğin APAPın toksik etkilerini azaltabileceği öne sürülmektedir. Bu hiperanaljezik etkinin moleküler mekanizmasını değerlendirmek için ileri çalışmalara ihtiyaç vardır.

Makale detayları

An Experimental Study on the Use of Exosomes against Acetaminophen-induced Uterine and Fallopian Tubes Damage in Rats

Yazarlar:

Ozge Goktepe

Yayıncı:

Erciyes University

Ana Başlıklar:

Veteriner Doğum ve Jinekoloji | Veteriner Histoloji ve Embriyolojisi

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 2, pp.127-133

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293731

pISSN:

1304-7280

Autophagy

acetaminophen

exosome

pain

ratAğrı

asetaminofen

eksozom

otofaji

sıçan

Sayı

Makale detayları

Yazarlar: Ozge Goktepe

Yayıncı: Erciyes University

Ana Başlıklar: Veteriner Doğum ve Jinekoloji | Veteriner Histoloji ve Embriyolojisi

Dergi: Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 2, pp.127-133

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293731

pISSN: 1304-7280

Sayı

Yazarlar:

Ozge Goktepe

Yayıncı:

Erciyes University

Ana Başlıklar:

Veteriner Doğum ve Jinekoloji | Veteriner Histoloji ve Embriyolojisi

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 2, pp.127-133

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293731

pISSN:

1304-7280